Rapid Bioassay Methods for Carcinogens and Modifiers of Hepatocarcinogenesis

1989 ◽  
Vol 19 (4) ◽  
pp. 385-415 ◽  
Author(s):  
Nobuyuki Ito ◽  
Katsumi Imaida ◽  
Ryohei Hasegawa ◽  
Hiroyuki Tsuda ◽  
Samuel M. Cohen
Keyword(s):  
1987 ◽  
Vol 252 (5) ◽  
pp. R1009-R1014 ◽  
Author(s):  
K. Matsui ◽  
T. Kimura ◽  
K. Ota ◽  
M. Shoji ◽  
M. Inoue ◽  
...  

A quantitative bioassay for the detection and quantification of atrial natriuretic polypeptides (ANPs) was developed in a pentobarbital-anesthetized rat. Ten percent mannitol in 0.9% saline was infused to achieve stable diuresis. The conductivity of the urine, urine flow, and blood pressure were continuously recorded. A bolus injection of synthetic alpha-human atrial natriuretic polypeptide (alpha-hANP) elicited dose-dependent increases in the urine conductivity, sodium excretion, and urine volume. Changes in the urine conductivity correlated significantly with the increase in sodium excretion. By use of changes in urine conductivity, biological ANP activity of crude rat atrial extract was determined. Atrial contents of ANP in spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) were 25.5 +/- 1.2 microgram per atrium (n = 4) and 25.1 +/- 0.8 (n = 5) in euhydration. They were increased to 27.0 +/- 1.1 micrograms (n = 4) and 29.3 +/- 1.3 (n = 5, P less than 0.05), after 5-day water deprivation, respectively. This assay procedure provides a good tool for rapid and quantitative determination of ANPs.


2005 ◽  
pp. 645-658
Author(s):  
Rettberg P. ◽  
C. Baumstark-Khan ◽  
E. Rabbow ◽  
G. Horneck

Weed Research ◽  
1971 ◽  
Vol 11 (2-3) ◽  
pp. 143-149 ◽  
Author(s):  
M. HOROWITZ and ◽  
NIRA HULIN
Keyword(s):  

2016 ◽  
Vol 54 (7) ◽  
pp. 1084-1089 ◽  
Author(s):  
Dániel Krüzselyi ◽  
Róbert Nagy ◽  
Péter G. Ott ◽  
Ágnes M. Móricz

1985 ◽  
Vol 16 (5) ◽  
pp. 457-462 ◽  
Author(s):  
Sarina Saltzman ◽  
Bruria Heuer
Keyword(s):  

Nature ◽  
1967 ◽  
Vol 215 (5103) ◽  
pp. 874-875 ◽  
Author(s):  
H. H. LUKE ◽  
T. E. FREEMAN
Keyword(s):  

1981 ◽  
Vol 27-27 (1) ◽  
pp. 577-582 ◽  
Author(s):  
N. J. Bauer ◽  
Ramon J. Seidler ◽  
M. D. Knittel
Keyword(s):  

1989 ◽  
Vol 82 (3) ◽  
pp. 706-715 ◽  
Author(s):  
Elizabeth E. Grafton-Cardwell ◽  
Jeffrey Granett ◽  
Thomas F. Leigh ◽  
Sherit A M. Normington
Keyword(s):  

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