Detection of Cellular Collapse in Albino Barley Leaves Inoculated with Erysiphe graminis hordei by UV-Fluorescence Microscopy

1976 ◽  
Vol 42 (5) ◽  
pp. 618-620 ◽  
Author(s):  
Shigeyuki MAYAMA ◽  
Jiko SHISHIYAMA
Keyword(s):  
Fluorescence Microscopy ◽  
Erysiphe Graminis ◽  
Uv Fluorescence ◽  
Erysiphe Graminis Hordei ◽  
Barley Leaves

Cytological studies of early stages of powdery mildew in barley and wheat. IX. Effect of various inorganic salts on the occurrence of autofluorescence at penetration sites of appressoria of Erysiphe graminis hordei

1983 ◽  
Vol 61 (8) ◽  
pp. 2181-2185 ◽  
Author(s):  
H. Kunoh ◽  
K. Yamamori ◽  
H. Ishizaki
Keyword(s):  
Powdery Mildew ◽  
Fluorescence Microscopy ◽  
Ethylenediaminetetraacetic Acid ◽  
Divalent Cations ◽  
Erysiphe Graminis ◽  
Inorganic Salts ◽  
Monovalent Cations ◽  
Enhanced Fluorescence ◽  
Enhancing Effect ◽  
Erysiphe Graminis Hordei

The effect of various inorganic salts on the occurrence of autofluorescence at penetration sites of appressoria of Erysiphe graminis hordei in barley coleoptiles was examined by fluorescence microscopy. Divalent cations such as Ca2+, Mg2+, and Mn2+ enhanced fluorescence to varying degrees. Among them, Ca2+ had the greatest enhancing effect; intense fluorescence occurred around penetration sites within 10 min after the inoculated coleoptiles were transferred from water to CaCl2 solution. However, other divalent cations such as Zn2+ and Fe2+ showed cytotoxicity to coleoptiles and did not elicit fluorescence. Monovalent cations such as K+, Na+, and Li+ did not show any enhancing effect independently. Anions seemed to be associated with enhancement of the flurorescence in combination with their related divalent cations but not independently. An observed elimination of fluorescence by ethylenediaminetetraacetic acid suggests that the enhancing effect of some of the divalent cations may be associated with their interaction with a substance(s) which fluoresces only in combination with these cations.

Cytological studies of the early stages of powdery mildew in barley and wheat. XI. Autofluorescence and haloes at penetration sites of appressoria of Erysiphe graminis hordei and Erysiphe pisi on barley coleoptiles

1985 ◽  
Vol 63 (9) ◽  
pp. 1535-1539 ◽  
Author(s):  
H. Kunoh ◽  
K. Kuno ◽  
H. Ishizaki
Keyword(s):  
Host Cell ◽  
Cell Walls ◽  
Time Course ◽  
Divalent Cations ◽  
Erysiphe Graminis ◽  
Erysiphe Pisi ◽  
Acid Dyes ◽  
Erysiphe Graminis Hordei ◽  
Barley Leaves

Cytological and physiological comparisons between autofluorescence and haloes which appeared at penetration sites of appressoria of Erysiphe graminis and Erysiphe pisi on barley coleoptiles were made using dye stains and fluorescence microscopy. Haloes induced by these fungi on barley coleoptiles were successfully detected by various dyes when the inoculated coleoptiles were treated with 70% ethanol at 80 °C for 30 min before staining. Responses of haloes on coleoptiles to basic and acid dyes were very similar to those on barley leaves which have been reported earlier. In time-course studies, haloes were not detected until at least 15 min after initiation of cytoplasmic aggregation. The occurrence of haloes was suppressed by supplemental Ca2+, but unaffected by K+, Na+, and Li+. Similar results were obtained in haloes induced by both fungi. Autofluorescent regions remained in situ after the inoculated coleoptile cells were plasmolyzed, suggesting that this phenomenon might be closely associated with host cell walls. As reported earlier, autofluorescence preceded the appearance of cytoplasmic aggregates by 1–10 min and, moreover, its appearance was enhanced by divalent cations such as Ca2+, Mg2+, and Mn2+, but not by monovalent cations such as K+, Na+, and Li+. Based on these results and earlier studies, it was concluded that the autofluorescence and haloes represented different responses in host cell walls to the penetration activities of both pathogenic and nonpathogenic fungi.

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