scholarly journals Elimination of Indian Citrus Ringspot Virus in Kinnow by Using Phytoproteins with Shoot‐tip Grafting

2017 ◽  
Vol 27 (1) ◽  
pp. 1-11
Author(s):  
Vikas Bishnoi ◽  
Anshul Goyal ◽  
HN Verma
Keyword(s):  
Shoot Tip ◽  
Ringspot Virus ◽  
Nodal Segments ◽  
Aqueous Extracts ◽  
Rt Pcr ◽  
Infected Mother ◽  
Shoot Tip Grafting ◽  
Mother Plants ◽  
Boerhaavia Diffusa

The combined beneficial effect of shoot‐tip grafting with phytotherapy of different phytoproteins obtained from roots of Boerhaavia diffusa and leaves of Clerodendrum aculeatum to eliminate Indian citrus ringspot virus (ICRSV) from Kinnow is reported. The study also reports the effect of these phytoproteins on the growth and proliferation of Kinnow explants when nodal segments from infected mother plants (confirmed by RT‐PCR) cultured in MS containing different concentrations of aqueous extracts of these individual phytoproteins. Shoot‐tips from these nodal sprouts were grafted on Citrus jambhiri under in vitro condition. Phytoproteins from C. aculeatum were found utmost effective in respect of elimination of 50% virus followed by B. diffusa (40%). In respect of promotion of growth and proliferation of nodal explants, B. diffusa was found most effective followed by C. aculeatum. The plants were considered virus‐free when they showed absence of ICRSV in both DAC‐ELISA and RT‐PCR tests.Plant Tissue Cult. & Biotech. 27(1): 1-11, 2017 (June)

In vitro production of Indian citrus ringspot virus (ICRSV) free kinnow plants employing phytotherapy coupled with shoot tip grafting

2007 ◽  
Vol 43 (3) ◽  
pp. 254-259 ◽  
Author(s):  
Sanjeev Sharma ◽  
Balwinder Singh ◽  
Gita Rani ◽  
A. A. Zaidi ◽  
V. Hallan ◽  
...  
Keyword(s):  
Shoot Tip ◽  
Ringspot Virus ◽  
In Vitro Production ◽  
Shoot Tip Grafting ◽  
Vitro Production

In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting

2007 ◽  
Vol 92 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Sanjeev Sharma ◽  
Balwinder Singh ◽  
Gita Rani ◽  
A. A. Zaidi ◽  
Vipin K. Hallan ◽  
...  
Keyword(s):  
Shoot Tip ◽  
Ringspot Virus ◽  
In Vitro Production ◽  
Shoot Tip Grafting ◽  
Vitro Production

Separation of citrus viroids by shoot-tip grafting in vitro

1990 ◽  
Vol 39 (3) ◽  
pp. 472-476 ◽  
Author(s):  
J. JUÁREZ ◽  
M. I. MOLINS ◽  
L. NAVARRO ◽  
N. DURAN-VILA
Keyword(s):  
Shoot Tip ◽  
Shoot Tip Grafting ◽  
Citrus Viroids

scholarly journals In vitro regeneration and somatic embryogenesis in citrus

2015 ◽  
Vol 24 (2) ◽  
pp. 247-262 ◽  
Author(s):  
El Sawy A Mohamed ◽  
Amina Gomaa ◽  
Nancy Danial
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Rt Pcr ◽  
Mother Plants

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT?PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV?free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant?dependant.Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December

RECOVERY OF VIRUS-FREE PEACH TREES FROM SELECTED CLONES BY SHOOT-TIP GRAFTING IN VITRO

1989 ◽  
pp. 77-84
Author(s):  
J. Juárez ◽  
J.M. Arregui ◽  
E. Camarasa ◽  
M. Cambra ◽  
G. Llácer ◽  
...  
Keyword(s):  
Shoot Tip ◽  
Shoot Tip Grafting ◽  
Peach Trees

RECOVERY OF VIRUS-FREE ALMOND PLANTS BY SHOOT-TIP GRAFTING IN VITRO

1992 ◽  
pp. 393-400 ◽  
Author(s):  
J. Juárez ◽  
E. Camarasa ◽  
C. Ortega ◽  
V. Ortega ◽  
J.M. Arregui ◽  
...  
Keyword(s):  
Shoot Tip ◽  
Shoot Tip Grafting

scholarly journals Micropropagation of Anubias barteri var. Nana from Shoot Tip Culture and the Analysis of Ploidy Stability

2012 ◽  
Vol 40 (2) ◽  
pp. 148 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Panyaros CHUNUI ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Plant Regeneration ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Culture ◽  
Ploidy Stability ◽  
Flow Cytometric ◽  
Regenerated Plants ◽  
Mother Plants

Plant regeneration of Anubias barteri var. Nana was achieved through organogenesis in shoot tip cultures. Multiple shoots were induced from cultured shoot tips on a modified MS (Murashige and Skoog, 1962) medium supplemented with BA and kinetin. The maximum green shoot numbers were best obtained on MS medium containing 3 mg/L BA with 5 shoots. Rooting in all regenerated shoots was promoted on MS medium devoid of plant growth regulators or kinetin singly. Acclimatization and survival when transferred to field conditions were shown to be 100% in the regenerated plants. Cytological and flow cytometric analyses of the mother plants and in vitro grown plants derived from 5 years old cultures showed no differences in ploidy level, they were all diploid (2n = 2x = 48) with a 2C peak indicating that ploidy alteration did not occur.

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