scholarly journals Uniform Orientation of Biotinylated Nanobody as an Affinity Binder for Detection of Bacillus thuringiensis (Bt) Cry1Ac Toxin

Toxins ◽  
2014 ◽  
Vol 6 (12) ◽  
pp. 3208-3222 ◽  
Author(s):  
Min Li ◽  
Min Zhu ◽  
Cunzheng Zhang ◽  
Xianjin Liu ◽  
Yakun Wan
2018 ◽  
Vol 61 ◽  
pp. 185-196 ◽  
Author(s):  
Karla I. Santos-Vigil ◽  
Damaris Ilhuicatzi-Alvarado ◽  
Ana L. García-Hernández ◽  
Juan S. Herrera-García ◽  
Leticia Moreno-Fierros

Toxins ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 538 ◽  
Author(s):  
Ma ◽  
Zhang ◽  
Xiao ◽  
Yang ◽  
Liu ◽  
...  

Bacillus thuringiensis Cry1Ac toxin binds to midgut proteins, as cadherin (CAD) and ABCC2 transporter, to form pores leading to larval death. In cell lines, co-expression of CAD and ABCC2 enhance Cry1Ac toxicity significantly, but the mechanism remains elusive. Here, we show that the expression of Helicoverpa armigera CAD (HaCAD-GFP) in Hi5 cells induces susceptibility to Cry1Ac and enhanced Cry1Ac toxicity when co-expressed with H. armigera ABCC2 (HaABCC2-GFP), since Cry1Ac toxicity increased 735-fold compared to Hi5 cells expressing HaCAD-GFP alone or 28-fold compared to HaABCC2-GFP alone. In contrast, the expression of the Spodoptera litura CAD (SlCAD-GFP) in Hi5 cells did not induce susceptibility to Cry1Ac nor it potentiated Cry1Ac toxicity with HaABCC2-GFP. To identify the CAD regions involved in the enhancement of Cry1Ac toxicity with ABCC2, the different CAD domains were replaced between SlCAD-GFP and HaCad-GFP proteins, and cytotoxicity assays were performed in Hi5 cells in the absence or presence of HaABCC2-GFP. The HaCAD toxin-binding region (TB), specifically the CAD repeat-11, was necessary to enhance Cry1Ac toxicity with ABCC2. We propose that CAD TB is involved in recruiting Cry1Ac to localize it in a good position for its interaction with the ABCC2, resulting in efficient toxin membrane insertion enhancing Cry1Ac toxicity.


2006 ◽  
Vol 139 (2) ◽  
pp. 223-233 ◽  
Author(s):  
Yasuyuki Shitomi ◽  
Tohru Hayakawa ◽  
Delwar M. Hossain ◽  
Masahiro Higuchi ◽  
Kazuhisa Miyamoto ◽  
...  

2006 ◽  
Vol 308 (1-2) ◽  
pp. 109-115 ◽  
Author(s):  
Rebekah C. Allen ◽  
Snezna Rogelj ◽  
Susan E. Cordova ◽  
Thomas L. Kieft

2009 ◽  
Vol 101 (2) ◽  
pp. 119-123 ◽  
Author(s):  
Wang Fa Xiang ◽  
Xia Li Qiu ◽  
Ding Xue Zhi ◽  
Zhao Xin Min ◽  
Lv Yuan ◽  
...  

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