Development of Optically Based pH Sensing Hydrogel and Controlled Nitric Oxide Release Polymer

Nitric oxide (NO) has been shown to be a potent vasodilator released from endothelial cells (EC) in large blood vessels, but NO release has not been examined in the capillary bed. Because the capillary bed represents the largest source of EC, it may be the largest source of vascular NO. In the present study, we used intravital microscopy to examine the effect of the NO synthase inhibitor, NG-nitro-L-arginine methyl ester (L-NAME), on the microvasculature of the rat extensor digitorum longus muscle. L-NAME (30 mM) applied locally to a capillary (300 micron(s) from the feeding arteriole) reduced red blood cell (RBC) velocity [VRBC; control VRBC = 238 +/- 58 (SE) micron/s; delta VRBC = -76 +/- 8%] and RBC flux (4.4 +/- 0.7 to 2.8 +/- 0.7 RBC/s) significantly in the capillary, but did not change feeding arteriole diameter (Dcon = 6.3 +/- 0.7 micron, delta D = 5 +/- 7%) or draining venule diameter (Dcon = 10.1 +/- 0.6 micron, delta D = 4 +/- 2%). Because of the VRBC change, the flux reduction was equivalent to an increased local hemoconcentration from 1.8 to 5 RBCs per 100 micron capillary length. L-NAME also caused an increase in the number of adhering leukocytes in the venule from 0.29 to 1.43 cells/100 micron. L-NAME (30 mM) applied either to arterioles or to venules did not change capillary VRBC. Bradykinin (BK) locally applied to the capillary caused significant increases in VRBC (delta VRBC = 111 +/- 23%) and in arteriolar diameter (delta D = 40 +/- 5%). This BK response was blocked by capillary pretreatment with 30 mM L-NAME (delta VRBC = -4 +/- 27%; delta D = 5 +/- 9% after BK). We concluded that NO may be released from capillary EC both basally and in response to the vasodilator BK. We hypothesize that 1) low basal levels of NO affect capillary blood flow by modulating local hemoconcentration and leukocyte adhesion, and 2) higher levels of NO (stimulated by BK) may cause a remote vasodilation to increase microvascular blood flow.

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Red Light‐Mediated Photoredox Catalysis Enables Self‐Reporting Nitric Oxide Release for Efficient Antibacterial Treatment

Angewandte Chemie International Edition ◽

10.1002/anie.202107155 ◽

2021 ◽

Author(s):

Jinming Hu ◽

Zhiqiang Shen ◽

Shaoqiu Zheng ◽

Shiyan Xiao ◽

Ruan Shen ◽

...

Keyword(s):

Nitric Oxide ◽

Red Light ◽

Antibacterial Treatment ◽

Photoredox Catalysis ◽

Nitric Oxide Release

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Enhanced nitric oxide release/synthesis in the posterior hypothalamus during nitroglycerin tolerance in rats

European Journal of Pharmacology ◽

10.1016/s0014-2999(03)01937-x ◽

2003 ◽

Vol 472 (3) ◽

pp. 179-187 ◽

Cited By ~ 7

Author(s):

Sheng-Xing Ma ◽

Ang Ji ◽

Marintan Pandjaitan ◽

Goodluck Ojije

Keyword(s):

Nitric Oxide ◽

Posterior Hypothalamus ◽

Nitric Oxide Release

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Nitric oxide release from human corpus cavernosum induced by a purified scorpion toxin

Urology ◽

10.1016/s0090-4295(03)00785-4 ◽

2004 ◽

Vol 63 (1) ◽

pp. 184-189 ◽

Cited By ~ 23

Author(s):

Cleber E. Teixeira ◽

Juliano F. de Oliveira ◽

Juliana S. Baracat ◽

Fernanda B.M. Priviero ◽

Cristina E. Okuyama ◽

...

Keyword(s):

Nitric Oxide ◽

Corpus Cavernosum ◽

Scorpion Toxin ◽

Nitric Oxide Release

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Nitric oxide release activated near-Infrared photothermal agent for synergistic tumor treatment

Biomaterials ◽

10.1016/j.biomaterials.2021.121017 ◽

2021 ◽

pp. 121017

Author(s):

Yunjian Xu ◽

Shiqi Wang ◽

Zhenjiang Chen ◽

Rui Hu ◽

Yihua Zhao ◽

...

Keyword(s):

Nitric Oxide ◽

Near Infrared ◽

Tumor Treatment ◽

Nitric Oxide Release

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