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2022 ◽  
Vol 295 ◽  
pp. 110805
Author(s):  
Toshio Shibuya ◽  
Yoshiki Shinto ◽  
Ryosuke Endo ◽  
Yosiaki Kitaya

2022 ◽  
Vol 186 ◽  
pp. 111835
Author(s):  
Lei Wang ◽  
Zisheng Luo ◽  
Mingyi Yang ◽  
Ze Liang ◽  
Ming Qi ◽  
...  
Keyword(s):  

2022 ◽  
Vol 198 ◽  
pp. 110005
Author(s):  
Yanan Wu ◽  
Huiqiang Gui ◽  
Liangwei Ma ◽  
Lei Zou ◽  
Xiang Ma
Keyword(s):  

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 519
Author(s):  
Maya Margaritova Zaharieva ◽  
Dimitrina Zheleva-Dimitrova ◽  
Snezhana Rusinova-Videva ◽  
Yana Ilieva ◽  
Anna Brachkova ◽  
...  

Small-scale photobioreactors (PBRs) in the inoculum stage were designed with internal (red or green) and external white LED light as an initial step of a larger-scale installation aimed at fulfilling the integral biorefinery concept for maximum utilization of microalgal biomass in a multifunctional laboratory. The specific growth rate of Scenedesmus obliquus (Turpin) Kützing biomass for given cultural conditions was analyzed by using MAPLE software. For the determination of total polyphenols, flavonoids, chlorophyll “a” and “b”, carotenoids and lipids, UHPLC-HRMS, ISO-20776/1, ISO-10993-5 and CUPRAC tests were carried out. Under red light growing, a higher content of polyphenols was found, while the green light favoured the flavonoid accumulation in the biomass. Chlorophylls, carotenoids and lipids were in the same order of magnitude in both samples. The dichloromethane extracts obtained from the biomass of each PBR synergistically potentiated at low concentrations (0.01–0.05 mg/mL) the antibacterial activity of penicillin, fluoroquinolones or oregano essential oil against the selected food-borne pathogens (Staphylococcus aureus, Escherichia coli and Salmonella typhimurium) without showing any in vitro cytotoxicity. Both extracts exhibited good cupric ion-reducing antioxidant capacity at concentrations above 0.042–0.08 mg/mL. The UHPLC-HRMS analysis revealed that both extracts contained long chain fatty acids and carotenoids thus explaining their antibacterial and antioxidant potential. The applied engineering approach showed a great potential to modify microalgae metabolism for the synthesis of target compounds by S. obliquus with capacity for the development of health-promoting nutraceuticals for poultry farming.


Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 527
Author(s):  
Agnieszka Krzymińska ◽  
Barbara Frąszczak ◽  
Monika Gąsecka ◽  
Zuzanna Magdziak ◽  
Tomasz Kleiber

The main focus of the study was to determine the content of phenolic acids, flavonoids, and organic acids in the flowers of Tagetes patula ‘Petite Gold’ and ‘Petite Orange’. The growth of the plants was assessed depending on the cultivation conditions. The above plants were illuminated with white light, whereas the ‘Petite Gold’ ones with white light enhanced with blue or red light. Both cultivars grew in a two-level-mineral compounds organic substrate. The research showed that the French marigold flowers were rich in phenolic compounds and organic acids. The ‘Petite Gold’ flowers had more bioactive compounds compared with the ‘Petite Orange’ flowers. Three flavonoids, 10 phenolic acids and seven organic acids were found in the ‘Petite Gold’ flowers. The artificial lighting used during the cultivation of the plants showed diversified influence on the content of organic compounds in their flowers. The measurements of the plants’ morphological traits and the number of inflorescences showed that illumination with red light resulted in a better effect. Large plants with numerous inflorescences grew in the substrate with a lower content of nutrients.


2022 ◽  
Vol 12 ◽  
Author(s):  
Qingping Ma ◽  
Laichao Song ◽  
Zhanhai Niu ◽  
Jingshan Li ◽  
Yu Wang ◽  
...  

“Huangjinya” is a light-sensitive albino variety and is widely cultivated in China. It has been proved that red light could promote the vegetable growth of plants. However, the mechanism of “Huangjinya” in response to a red light is unclear. This study used high-throughput sequencing technology to analyze the transcriptome of tender shoots of “Huangjinya” under the white and red light supplement conditions. At the same time, liquid chromatography tandem mass spectrometry (LC-MS) was used to analyze metabolite changes under different light conditions. Transcriptome analysis revealed that a total of 174 differentially expressed genes (DEGs) were identified after the red light supplement. Kyoto encyclopedia of genes and genomes (KEGG) classification indicated that amino acid metabolism enriched the most DEGs. In addition, two phenylpropanoid metabolism-related genes and five glutathione S-transferase genes (CsGSTs) were found to be expressed differently. Metabolome analysis revealed that 193 differential metabolites were obtained. Being the same as transcriptome analysis, most differential metabolites were enriched in amino acids, sweet and umami tasting amino acids were increased, and bitter-tasting amino acids were decreased after the red light supplement. In summary, red light supplementary treatment may be propitious to the quality of “Huangjinya” due to its regulatory effect on amino acid metabolism. Also, CsGSTs involved phenylpropanoid metabolism contributed to tea quality changes in “Huangjinya.”


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