Theoretical Study on the Mechanism of Ethanol Oxidation to Acetaldehyde Catalyzed by Liver Alcohol Dehydrogenase

2010 ◽  
Vol 31 (9) ◽  
pp. 1167-1171
Author(s):  
Xiaoying LI ◽  
Changsheng WANG
Keyword(s):  
Alcohol Dehydrogenase ◽  
Ethanol Oxidation ◽  
Theoretical Study ◽  
Liver Alcohol Dehydrogenase

scholarly journals Inhibition of Horse Liver Alcohol Dehydrogenase by Methyltin Compounds

2005 ◽  
Vol 3 (3-4) ◽  
pp. 191-199 ◽  
Author(s):  
Pavel V. Bychkov ◽  
Tatyana N. Shekhovtsova ◽  
Elena R. Milaeva
Keyword(s):  
Alcohol Dehydrogenase ◽  
Ethanol Oxidation ◽  
Calculated Data ◽  
Enzyme Protein ◽  
Horse Liver Alcohol Dehydrogenase ◽  
Tin Compounds ◽  
Liver Alcohol Dehydrogenase ◽  
Horse Liver ◽  
Oxidative Agents ◽  
Slight Inhibition

The study of inorganic tin (SnCl2, SnCl4) and methyltin compounds (MeSnCl3, Me2SnCI2, Me3SnCl) effects on the enzymatic activity of alcohol dehydrogenase (ADH) in the reaction of ethanol oxidation has been carried out. The experimental results of the study show that inorganic tin and methyltin substances induce slight inhibition of the catalytic activity of horse liver alcohol dehydrogenase (HLADH), unable to be improved during pre-incubation with the enzyme. The conditions for carrying out the kinetic investigation of the mentioned phenomenon were optimized and as it turned out the mechanism of methyltin trichloride action, as the most effective methyltin inhibitor, is more complex than the proposed interaction of the metal atom with SH-groups of the enzyme protein. It was demonstrated that the tin compounds act in the same manner as methylmercury compounds and might serve as oxidative agents towards the co-enzyme NADH. Kinetic data on MeSnCl3were calculated. Data acquired on NAD-dependent ADH from horse liver and those regarding NAD-dependent LDH from sturgeon liver were compared.

EXAFS INVESTIGATION OF THE STRUCTURAL SITE OF LIVER ALCOHOL DEHYDROGENASE

1986 ◽  
Vol 47 (C8) ◽  
pp. C8-1165-C8-1168
Author(s):  
M. ZEPPEZAUER ◽  
C. HAAS ◽  
W. MARET ◽  
C. HERMES ◽  
R. F. PETTIFER
Keyword(s):  
Alcohol Dehydrogenase ◽  
Liver Alcohol Dehydrogenase ◽  
Structural Site
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