IN VITRO MODEL OF A HYPERTROPHIC SCAR

2020 ◽  
Author(s):  
Валериан Сергеевич Шадрин ◽  
Петр Михайлович Кожин ◽  
Олеся Олеговна Шошина ◽  
Александр Леонидович Русанов
Keyword(s):  
Wound Healing ◽  
Hypertrophic Scar ◽  
In Vitro Model ◽  
World Health ◽  
Hypertrophic Scarring ◽  
Actual Problem ◽  
Vitro Model

Изучение нарушения ранозаживления, приводящего к развитию гиперпластических процессов, является актуальной задачей мирового здравоохранения. Для более детального изучения данной проблематики предложена новая in vitro модель, которая является более удобной для применения и при этом отражает признаки, характерные для гипертрофического рубцевания. The study of wound healing disorders leading to the development of hyperplastic processes is an actual problem of world health. A new in vitro model is proposed for a more detailed study of this problem. This model is more convenient for use and at the same time reflects the signs characteristic of hypertrophic scarring.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

099 "Smart" Targeting of Anti?Scarring Agents in an in vitro Model of Retinal Wound Healing

2004 ◽  
Vol 12 (2) ◽  
pp. A26-A26
Author(s):  
F E Dhawahir ◽  
C Sheridan ◽  
D Kent ◽  
D Wong ◽  
I Grierson ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Insulin catalyzes the curcumin-induced wound healing: An in vitro model for gingival repair

2012 ◽  
Vol 44 (4) ◽  
pp. 458 ◽  
Author(s):  
AnilK Balapure ◽  
Jaya Dixit ◽  
Divya Lodha ◽  
Vishal Ranjan ◽  
Ramesh Sharma ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Bioelectric modulation of wound healing in a 3D in vitro model of tissue-engineered bone

Biomaterials ◽  
2013 ◽  
Vol 34 (28) ◽  
pp. 6695-6705 ◽  
Author(s):  
Sarah Sundelacruz ◽  
Chunmei Li ◽  
Young Jun Choi ◽  
Michael Levin ◽  
David L. Kaplan
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
3D In Vitro Model ◽  
Vitro Model

Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix

1997 ◽  
Vol 110 (7) ◽  
pp. 861-870 ◽  
Author(s):  
D. Greiling ◽  
R.A. Clark
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Collagen Gel ◽  
Fibrin Clot ◽  
Dependent Manner ◽  
Fibrin Gel ◽  
Concentration Dependent Manner ◽  
Vitro Model ◽  
Early Wound

After injury, the wound space is filled with a fibrin/fibronectin clot containing growth factors released by platelets and monocytes. In response to these factors, fibroblasts migrate into the fibrin clot and contribute to the formation of granulation tissue. The functional mechanisms allowing fibroblasts to leave the collagenous matrix of normal connective tissue and invade the provisional matrix of the fibrin clot have not been fully defined. To investigate these mechanisms we established a new in vitro model which simulates specific aspects of early wound healing, that is, the migration of fibroblasts from a three-dimensional collagen matrix into a fibrin clot. This transmigration could be induced by physiological concentrations of platelet releasate or platelet-derived growth factor BB (PDGF-BB) in a concentration-dependent manner. At 24 hours irradiated fibroblasts invaded the fibrin gel almost as well as non-irradiated cells, indicating that transmigration was independent of proliferation. Plasminogen and its activators appear to be necessary for invasion of the fibrin clot since protease inhibitors decreased the amount of migration. These serine proteases, however, were not necessary for exit from the collagen gel as fibroblasts migrated out of the collagen gel onto a surface coated with fibrin fibrils even in the presence of inhibitors. Removal of fibronectin (FN) from either the collagen gel or the fibrin gel markedly decreased the number of migrating cells, suggesting that FN provides a conduit for transmigration. Cell movement in the in vitro model was inhibited by RGD peptide, and by monoclonal antibodies against the subunits of the alpha5 beta1 and alpha v beta3 integrin receptor. Thus, the functional requirements for fibroblast transmigration from collagen-rich to fibrin-rich matrices, such as occurs in early wound healing, have been partially defined using an in vitro paradigm of this important biologic process.

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

10.3791/61037 ◽  
2020 ◽  
Author(s):  
Chen Fan ◽  
Lay Keng Priscilla Lim ◽  
Zihao Wu ◽  
Bhavya Sharma ◽  
Shi Qi Gan ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Macromolecular Crowding ◽  
Hypertrophic Scarring ◽  
Vitro Model

scholarly journals 3D In vitro model of the re-epithelialization phase in the wound-healing process

2017 ◽  
Vol 27 (5) ◽  
pp. 460-462 ◽  
Author(s):  
Nathalie Deshayes ◽  
Fabienne Bloas ◽  
Florian Boissout ◽  
Jennifer Lecardonnel ◽  
Maryline Paris
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Healing Process ◽  
Wound Healing Process ◽  
3D In Vitro Model ◽  
Vitro Model

Mechanical Strain Alters Gene Expression in an in Vitro Model of Hypertrophic Scarring

2005 ◽  
Vol 55 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Christopher A. Derderian ◽  
Nicholas Bastidas ◽  
Oren Z. Lerman ◽  
Kirit A. Bhatt ◽  
Shin-E Lin ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Model ◽  
Mechanical Strain ◽  
Hypertrophic Scarring ◽  
Vitro Model
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