Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology

10.3791/59461 ◽  
2019 ◽  
Author(s):  
Julia K. Sunstrum ◽  
Wataru Inoue
Keyword(s):  
Patch Clamp ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Patch Clamp Electrophysiology

scholarly journals Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology

Neuron ◽  
2017 ◽  
Vol 95 (5) ◽  
pp. 1048-1055.e3 ◽  
Author(s):  
Luca A. Annecchino ◽  
Alexander R. Morris ◽  
Caroline S. Copeland ◽  
Oshiorenoya E. Agabi ◽  
Paul Chadderton ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Whole Cell ◽  
Two Photon ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Patch Clamp Electrophysiology ◽  
Robotic Automation

scholarly journals Automated whole-cell patch-clamp electrophysiology of neurons in vivo

2012 ◽  
Vol 9 (6) ◽  
pp. 585-587 ◽  
Author(s):  
Suhasa B Kodandaramaiah ◽  
Giovanni Talei Franzesi ◽  
Brian Y Chow ◽  
Edward S Boyden ◽  
Craig R Forest
Keyword(s):  
Patch Clamp ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Patch Clamp Electrophysiology

scholarly journals Whole Cell PatchClamp Electrophysiology in Opsin-Expressing Brain Slice with Visible Lights or UCNPs

2021 ◽  
Author(s):  
Yueguang Si ◽  
Yiting Wang
Keyword(s):  
Patch Clamp ◽  
Brain Slice ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Mice Brain ◽  
Patch Clamp Electrophysiology

Abstract It describes the flow of whole cell patch-clamp electrophysiology in mice brain slice.

Whole Cell Patch Clamp of Dispersed Human Islet Cells v1

2021 ◽  
Author(s):  
Xiaoqing Dai ◽  
Austin Bautista ◽  
Patrick E Macdonald
Keyword(s):  
Patch Clamp ◽  
Single Cell ◽  
Pancreatic Islets ◽  
Islet Cells ◽  
Single Cells ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Patch Clamp Electrophysiology ◽  
Channel Currents

Cells use exocytosis to secrete a wide variety of molecules, including proteins, hormones, and neurotransmitters. Exocytosis can be monitored at the single-cell level by using patch-clamp electrophysiology to measure changes in membrane capacitance as vesicles fuse with the cell membrane and release their content. Dispersion of pancreatic islets into single cells allows for individual characterization of electrophysiological characteristics and allows for collection of cellular content for recovery of full-length transcriptomes by use of Smart-seq2. Described in this protocol is the dispersion of pancreatic islets into single cells followed by whole-cell patch clamp electrophysiology which includes parameters representing cell size, exocytosis, sodium channel currents, and calcium channel currents. Cells are then collected individually after recording to be processed for single-cell RNA sequencing.

Whole-cell patch-clamp recordings from respiratory neurons in neonatal rat brainstem in vitro

1992 ◽  
Vol 134 (2) ◽  
pp. 153-156 ◽  
Author(s):  
Jeffrey C. Smith ◽  
Klaus Ballanyi ◽  
Diethelm W. Richter
Keyword(s):  
Patch Clamp ◽  
Neonatal Rat ◽  
Respiratory Neurons ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Rat Brainstem

Error analysis of Cm measurement under the whole-cell patch-clamp recording

2010 ◽  
Vol 185 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Hao Zhang ◽  
Anlian Qu ◽  
Jie Luo ◽  
Jie Luo
Keyword(s):  
Patch Clamp ◽  
Error Analysis ◽  
Patch Clamp Recording ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp
Sign in / Sign up

Export Citation Format

Share Document