scholarly journals Identification of Neighbouring Components in the Quaternary Eukaryotic Protein Synthesis Initiation Complex, eIF-2.GTP.Met-tRNAf.small ribosomal subunit.

1981 ◽  
Vol 35b ◽  
pp. 57-59 ◽  
Author(s):  
Odd Nygård ◽  
Peter Westermann ◽  
Tore Hultin ◽  
Gian Maria Pacifici ◽  
Anders Rane
Keyword(s):  
Protein Synthesis ◽  
Ribosomal Subunit ◽  
Initiation Complex ◽  
Eukaryotic Protein ◽  
Protein Synthesis Initiation

scholarly journals Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways

2016 ◽  
pp. gkw1251 ◽  
Author(s):  
Jorge P. López-Alonso ◽  
Attilio Fabbretti ◽  
Tatsuya Kaminishi ◽  
Idoia Iturrioz ◽  
Letizia Brandi ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Initiation Complex ◽  
Eukaryotic Protein ◽  
Protein Synthesis Initiation

scholarly journals The Oxazolidinone Linezolid Inhibits Initiation of Protein Synthesis in Bacteria

1998 ◽  
Vol 42 (12) ◽  
pp. 3251-3255 ◽  
Author(s):  
Steve M. Swaney ◽  
Hiroyuki Aoki ◽  
M. Clelia Ganoza ◽  
Dean L. Shinabarger
Keyword(s):  
Protein Synthesis ◽  
Complex Formation ◽  
Antimicrobial Agents ◽  
Ribosomal Subunit ◽  
Multidrug Resistant ◽  
Binary Complex ◽  
Initiation Complex ◽  
Ribosomal Subunits ◽  
Bacterial Translation

ABSTRACT The oxazolidinones represent a new class of antimicrobial agents which are active against multidrug-resistant staphylococci, streptococci, and enterococci. Previous studies have demonstrated that oxazolidinones inhibit bacterial translation in vitro at a step preceding elongation but after the charging ofN-formylmethionine to the initiator tRNA molecule. The event that occurs between these two steps is termed initiation. Initiation of protein synthesis requires the simultaneous presence of N-formylmethionine-tRNA, the 30S ribosomal subunit, mRNA, GTP, and the initiation factors IF1, IF2, and IF3. An initiation complex assay measuring the binding of [3H]N-formylmethionyl-tRNA to ribosomes in response to mRNA binding was used in order to investigate the mechanism of oxazolidinone action. Linezolid inhibited initiation complex formation with either the 30S or the 70S ribosomal subunits fromEscherichia coli. In addition, complex formation withStaphylococcus aureus 70S tight-couple ribosomes was inhibited by linezolid. Linezolid did not inhibit the independent binding of either mRNA or N-formylmethionyl-tRNA toE. coli 30S ribosomal subunits, nor did it prevent the formation of the IF2–N-formylmethionyl-tRNA binary complex. The results demonstrate that oxazolidinones inhibit the formation of the initiation complex in bacterial translation systems by preventing formation of theN-formylmethionyl-tRNA–ribosome–mRNA ternary complex.

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