Restriction fragment analysis of hordein genes in western Canadian two-rowed barleys

1995 ◽  
Vol 75 (1) ◽  
pp. 191-193
Author(s):  
S. J. Molnar ◽  
A. McKay
Keyword(s):  
Restriction Fragment ◽  
Cultivar Identification ◽  
Protein Pattern ◽  
Gene Families ◽  
Protein Patterns ◽  
Fragment Analysis ◽  
Banding Patterns ◽  
Barley Cultivars ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms (RFLP) at the hordein loci were compared with hordein protein patterns for discrimination of barley cultivars. RFLP banding patterns documented extensive polymorphism for B and C hordein gene families in eight closely related western Canadian two-rowed barley cultivars, five parental cultivars and a U.K. cultivar. RFLP results were compared with published protein pattern data on the same cultivars. The power to discriminate cultivars by the two methods is similar. Key words: RFLP, hordein, barley, Hordeum vulgare, cultivar identification

Restriction fragment analysis of ribosomal and hordein genes in eastern Canadian two-rowed barleys

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 298-302 ◽  
Author(s):  
Stephen J. Molnar ◽  
Adèle McKay
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Seed Storage Protein ◽  
Restriction Fragment Length Polymorphisms ◽  
Fragment Analysis ◽  
Restriction Fragment Analysis ◽  
Barley Cultivars ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms (RFLPs) have been advocated as useful genetic markers for a variety of genetic and plant breeding applications. Among barley cultivars, RFLPs have been reported in three well-characterized multigene families: at the hordein seed storage protein loci, at the 18S + 5.8S + 26S ribosomal DNA loci, and for 5S ribosomal DNA. The present study sampled RFLPs in these three gene families in the five major eastern Canadian two-rowed barley cultivars. The target sequences constitute at least six loci distributed over at least three chromosomes but represent a very small percentage of the total genome. The five cultivars studied share common alleles at the 18S + 5.8S + 26S ribosomal loci Rrn 1 and Rrn 2 and at the 5S ribosomal loci. This may reflect a fairly narrow genetic base for these cultivars. Moderate amounts of polymorphism were detected at the Hor 1 and Hor 2 loci, each of which comprises a small multigene family. Restriction fragment length polymorphism complemented protein polymorphism at the hordein loci, arguing for their joint use for cultivar identification or similar purposes.Key words: barley, restriction fragment analysis, restriction fragment length, polymorphisms, hordeins, ribosomal DNA.

scholarly journals IDENTIFICATION OF ROSE CULTIVARS BY RESTRICTION FRAGMENT LENGTH POLYMORPHISMS

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 484b-484
Author(s):  
Sriyani Rajapakse ◽  
Mark Hubbard ◽  
Albert Abbott ◽  
Robert Ballard ◽  
John Kelly
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Genomic Dna ◽  
Cultivar Identification ◽  
Restriction Fragment Length Polymorphisms ◽  
Repeated Sequences ◽  
Dna Library ◽  
Dichotomous Key ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Restriction Fragment Length Polymorphisms (RFLPs) were investigated in rose cultivars as a means of reliable cultivar identification. A random genomic DNA library was generated by shotgun cloning HindIII digested fragments of DNA extracted from rose cultivar Confection into pUC8 plasmid of Escherichia coli strain JM 83. Compared to genomic clones carrying low or highly repeated sequences, clones with moderately repeated sequences were most effective in cultivar identification. These clones were identified by hybridizing rose DNA fragments from the library with genomic DNA from `Confection'. Clones with moderately repeated copy sequences were used as probes to detect the presence of RFLPs by Southern hybridization of EcoRI digested genomic DNA of various rose cultivars. Several of these probes have revealed RFLPs useful in cultivar identification. By using a combination of two or more of these probes most of the rose cultivars compared at this time can be identified. A dichotomous key useful in identification of rose cultivars was prepared from RFLPs displayed by 3A9 probe.

scholarly journals Genetic Similarity Among Brassica oleracea L. Genotypes as Measured by Restriction Fragment Length Polymorphisms

1993 ◽  
Vol 118 (2) ◽  
pp. 298-303 ◽  
Author(s):  
James Nienhuis ◽  
Mary K. Slocum ◽  
Dawn A. DeVos ◽  
Roger Muren
Keyword(s):  
Brassica Oleracea ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Genetic Similarity ◽  
Fragment Length Polymorphism ◽  
Pedigree Information ◽  
Banding Patterns ◽  
Length Polymorphisms ◽  
Wide Range ◽  
Restriction Fragment Length

Genetic similarities were calculated among 89 Brassica oleracea L. genotypes, which included 62 broccolis (var. italica), 16 cauliflowers (var. botrytis), and 11 cabbages (var. capitata). These entries represented a wide range of commercially available germplasm, including open-pollinated cultivars, commercial hybrids, the inbred parents of several hybrid cultivars, and 27 entries that were provided as unknowns. Sixteen random genomic clones were used as probes in Southern hybridizations to detect restriction fragment length polymorphism (RFLP). From each of the random probes, an average of four polymorphic bands were classified as to their presence or absence for each genotype. The genetic similarity between ail pairs of genotypes was calculated. A multidimensional scaling (MDS) plot indicated that the broccoli, cauliflower, and cabbage groups were clustered with very little overlap. Within groups, genetic similarity corresponded to relationships based on available pedigree information. Comparison of banding patterns between hypothetical and actual hybrids was used to correctly identify the parents of several parent-hybrid combinations. The RFLP pattern of a hybrid and one of the parents (female) were used to predict the genotype and identity of the other parent (male).

scholarly journals Restriction Fragment Length Polymorphisms in Rose and Their Use for Cultivar Identification

HortScience ◽  
1992 ◽  
Vol 27 (2) ◽  
pp. 172-173 ◽  
Author(s):  
M. Hubbard ◽  
J. Kelly ◽  
S. Rajapakse ◽  
A. Abbott ◽  
R. Ballard
Keyword(s):  
Genetic Markers ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Patent Protection ◽  
Cultivar Identification ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Patent Rights ◽  
New Cultivars ◽  
Restriction Fragment Length

We have identified cloned rose DNA fragments that detect restriction fragment length polymorphisms (RFLP) in rose (Rosa ×hybrida) cultivars. RFLP can be used as genetic markers for identification, certification, and patent protection. By comparing RFLP patterns for each of six probes, we have been able to characterize eight cultivars. These results confirm that RFLP analyses are useful for rose cultivar identification and may provide a means for protecting patent rights to new cultivars.

scholarly journals A genetic linkage map for the domesticated silkworm, Bombyx mori, based on restriction fragment length polymorphisms

1995 ◽  
Vol 66 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Jinrui Shi ◽  
David G. Heckel ◽  
Marian R. Goldsmith
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Genetic Maps ◽  
Crossing Over ◽  
Linkage Groups ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

SummaryWe present data for the initial construction of a molecular linkage map for the domesticated silkworm, Bombyx mori, based on 52 progeny from an F2 cross from a pair mating of inbred strains p50 and C108, using restriction fragment length polymorphisms (RFLPs). The map contains 15 characterized single copy sequences, 36 anonymous sequences derived from a follicular cDNA library, and 10 loci corresponding to a low copy number retrotransposon, mag. The 15 linkage groups and 8 ungrouped loci account for 23 of the 28 chromosomes and span a total recombination length of 413 cM; 10 linkage groups were correlated with established classic genetic maps. Scoring data from Southern blots were analysed using two Pascal programs written specifically to analyse linkage data in Lepidoptera, where females are the heterogametic sex and have achiasmatic meiosis (no crossing-over). These first examine evidence for linkage by calculating the maximum lod score under the hypothesis that the two loci are linked over the likelihood under the hypothesis that the two loci assort independently, and then determine multilocus linkage maps for groups of putatively syntenic loci by calculating the maximum likelihood estimate of the recombination fractions and the log likelihood using the EM algorithm for a specified order of loci along the chromosome. In addition, the possibility of spurious linkage was exhaustively tested by searching for genotypes forbidden by the absence of crossing-over in one sex.

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