Bt rice evaluation and deployment strategies

GM Crops ◽  
2011 ◽  
Vol 2 (3) ◽  
pp. 135-137 ◽  
Author(s):  
P. Manimaran ◽  
G. Ramkumar ◽  
M. Mohan ◽  
S. K. Mangrauthia ◽  
A.P. Padmakumari ◽  
...  
Keyword(s):  
Bt Rice

Effects of triazophos on biochemical substances of transgenic Bt rice and its nontarget pest Nilaparvata lugens Stål under elevated CO2

2013 ◽  
Vol 107 (2) ◽  
pp. 188-199 ◽  
Author(s):  
Lin-Quan Ge ◽  
Jin-Cai Wu ◽  
Yu-Cheng Sun ◽  
Fang Ouyang ◽  
Feng Ge
Keyword(s):  
Elevated Co2 ◽  
Nilaparvata Lugens ◽  
Bt Rice ◽  
Nilaparvata Lugens Stål

Achieving successful deployment of Bt rice

2004 ◽  
Vol 9 (6) ◽  
pp. 286-292 ◽  
Author(s):  
S MINGHIGH
Keyword(s):  
Bt Rice

Multiplex, Construct-Specific, and Real-Time PCR-Based Analytical Methods for Bt Rice with cry1Ac Gene

2012 ◽  
Vol 95 (1) ◽  
pp. 186-194 ◽  
Author(s):  
Gurinder Jit Randhawa ◽  
Monika Singh
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Analytical Methods ◽  
Camv 35S Promoter ◽  
35S Promoter ◽  
Pcr Assay ◽  
Cry1ac Gene ◽  
Camv 35S ◽  
Bt Rice ◽  
Pcr Assays

Abstract Qualitative and quantitative analytical methods based on PCR for Bacillus thuringiensis (Bt) rice hybrid, namely, MRP 5401 Bt expressing a modified version of the Bt cry1Ac gene, are reported here. Multiplex PCR assays were developed to target the cry1Ac transgene, Cauliflower mosaic virus (CaMV) 35S promoter, Agrobacterium tumefaciens nopaline synthase (nos) terminator, the neomycin phosphotransferase II (nptII) marker gene, and an endogenous α-tubulin (TubA) gene in Bt rice. The 3.178 kb region of inserted gene construct comprising the region of the CaMV 35S promoter and cry1Ac gene was amplified, and the construct integrity was confirmed by the nested PCR. The LOD for cry1Ac gene-specific simplex PCR was 0.01%, as established using Bt rice DNA dilutions with 100, 10, 1.0, 0.1, 0.05, 0.01, and 0.001% genetically modified trait. A real-time PCR assay was also developed to quantify the cry1Ac gene. The method performance of the reported real-time PCR assay was in line with the acceptance criteria of Codex Alimentarius Commission ALINORM 10/33/23, with LOD and LOQ values of 0.05%. The reliable PCR assays prior to commercial release of Bt rice would facilitate efficient regulatory compliance for identification of genetic trait, labeling requirements, and effective risk assessment and management. They could also address consumers' concerns and legal disputes that may arise.

Consumption of Bt rice pollen expressing Cry2Aa does not cause adverse effects on adult Chrysoperla sinica Tjeder (Neuroptera: Chrysopidae)

2012 ◽  
Vol 61 (3) ◽  
pp. 246-251 ◽  
Author(s):  
Yuanyuan Wang ◽  
Yunhe Li ◽  
Jörg Romeis ◽  
Xiuping Chen ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Bt Rice

Bt rice producing Cry1C protein does not have direct detrimental effects on the green lacewingChrysoperla sinica(Tjeder)

2014 ◽  
Vol 33 (6) ◽  
pp. 1391-1397 ◽  
Author(s):  
Yunhe Li ◽  
Xiuping Chen ◽  
Long Hu ◽  
Jörg Romeis ◽  
Yufa Peng
Keyword(s):  
Bt Rice

Effectiveness in the field of Bt rice lines against target pests under various cultural regimes

2015 ◽  
Vol 156 (3) ◽  
pp. 211-219
Author(s):  
Yajun Yang ◽  
Hailiang Han ◽  
Hongxing Xu ◽  
Xusong Zheng ◽  
Junce Tian ◽  
...  
Keyword(s):  
Bt Rice

Comparison of transgenic Bt rice and their non-Bt counterpart in yield and physiological response to drought stress

2018 ◽  
Vol 217 ◽  
pp. 45-52 ◽  
Author(s):  
Yang Jiang ◽  
Lin Ling ◽  
Lingli Zhang ◽  
Kangxu Wang ◽  
Xuexue Li ◽  
...  
Keyword(s):  
Drought Stress ◽  
Physiological Response ◽  
Bt Rice
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