scholarly journals Peer Review #1 of "An application of PCR-RFLP species identification assay for environmental DNA detection (v0.1)"

2019 ◽  
Keyword(s):  
Peer Review ◽  
Species Identification ◽  
Dna Detection ◽  
Environmental Dna ◽  
Pcr Rflp

scholarly journals Effects of preservation strategies on environmental DNA detection and quantification using ddPCR

2021 ◽  
Author(s):  
Quentin Mauvisseau ◽  
David Halfmaerten ◽  
Sabrina Neyrinck ◽  
Alfred Burian ◽  
Rein Brys
Keyword(s):  
Dna Detection ◽  
Environmental Dna ◽  
Detection And Quantification

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

2011 ◽  
Vol 14 (2) ◽  
pp. 285-286 ◽  
Author(s):  
J. Karakulska ◽  
A. Pobucewicz ◽  
P. Nawrotek ◽  
M. Muszyńska ◽  
A. Furowicz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Species Identification ◽  
Genetic Relationship ◽  
Molecular Typing ◽  
Rapd Analysis ◽  
Milk Samples ◽  
Rapd Pcr ◽  
Pcr Rflp ◽  
Mastitic Milk ◽  
Coa Gene

Molecular typing ofStaphylococcus aureusbased on PCR-RFLP ofcoagene and RAPD analysisThe aim of this study was molecular identification ofS. aureusstrains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains thegapgene (930 bp) was amplified, which enabled their affiliation to theStaphylococcusgenus to be established. PCR-RFLP withAluI endonuclease of thegapgene as well asnuc(450 bp) andcoa(1130 bp) gene amplification allowed preciseS. aureusspecies identification. One hundred percent of the genetic relationship between strains was establishedviaRAPD-PCR and coa-typing.

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