scholarly journals Stability indicating RP-HPLC method for simultaneous determination of pyrimethamine and sulfamethoxypyrazine in pharmaceutical formulation: Application to method validation

2020 ◽  
Vol 10 (2) ◽  
pp. 49-55
Keyword(s):  
Simultaneous Determination ◽  
Method Validation ◽  
Hplc Method ◽  
Pharmaceutical Formulation ◽  
Stability Indicating ◽  
Rp Hplc

Stability-Indicating TLC-Densitometric and HPLC Methods for the Simultaneous Determination of Piracetam and Vincamine in the Presence of Their Degradation Products

2016 ◽  
Vol 99 (6) ◽  
pp. 1490-1498 ◽  
Author(s):  
Amal B Ahmed ◽  
Maha M Abdelrahman ◽  
Nada S Abdelwahab ◽  
Fathy M Salama
Keyword(s):  
Simultaneous Determination ◽  
Degradation Products ◽  
Hplc Method ◽  
Pharmaceutical Formulation ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Accuracy And Precision ◽  
Array Detection ◽  
Assay Methods

Abstract Newly established TLC-densitometric and RP-HPLC methods were developed and validated for the simultaneous determination of Piracetam (PIR) and Vincamine (VINC) in their pharmaceutical formulation and in the presence of PIR and VINC degradation products, PD and VD, respectively. The proposed TLC-densitometric method is based on the separation and quantitation of the studied components using a developing system that consists of chloroform–methanol–glacial acetic acid–triethylamine (8 + 2 + 0.1 + 0.1, v/v/v/v) on TLC silica gel 60 F254 plates, followed by densitometric scanning at 230 nm. On the other hand, the developed RP-HPLC method is based on the separation of the studied components using an isocratic elution of 0.05 M KH2PO4 (containing 0.1% triethylamine adjusted to pH 3 with orthophosphoric acid)–methanol (95 + 5, v/v) on a C8 column at a flow rate of 1 mL/min with diode-array detection at 230 nm. The developed methods were validated according to International Conference on Harmonization guidelines and demonstrated good accuracy and precision. Moreover, the developed TLC-densitometric and RP-HPLC methods are suitable as stability-indicating assay methods for the simultaneous determination of PD and VD either in bulk powder or pharmaceutical formulation. The results were statistically compared with those obtained by the reported RP-HPLC method using t- and F-tests.

scholarly journals STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION

2017 ◽  
Vol 62 (3) ◽  
pp. 3572-3577
Author(s):  
SYED NAEEM RAZZAQ ◽  
MUHAMMAD ASHFAQ ◽  
IRFANA MARIAM ◽  
ISLAM ULLAH KHAN ◽  
SYED SALEEM RAZZAQ ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Binary Combination
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