The chief results at which I have arrived may be summarised as follows:
There is an intracellular network present in the striped muscle-fibre of Dytiscus, the Bee, Prog, Lobster, Crayfish, and Rat, which may be most clearly demonstrated by certain methods of gold staining. The network alone is stained by the reduced gold, and, owing to this differentiation, is plainly visible even with comparatively low powers. This network may be demonstrated, though not so completely, in the living fibre, and in acetic and osmic acid preparations.
Crossing the fibre transversely, united to the sarcolemnia, and more or less separating the muscle-fibre into compartments, are network partitions--the transverse networks.
Running longitudinally down each compartment, and joining the dots at the intersections of the fibres of the transverse network, are a series of fine rods. The arrangement of this network will be made evident by reference to diagrams 1, 2, 3, and 4.
This network consists of an isotropous material, and is more highly refractile than the rest of the muscle substance, which is anisotropous. This network serves to explain the transverse striation and other complicated appearances presented by the muscle-fibre, and brings into harmony many of the conflicting statements of histologists on this subject.