Explore the Structural Changes of Nitrogen-fixing Microorganisms of Rhizosheath During the Growth of Stipagrostis Pennata in the Desert

Purpose: Rhizosheath is an adaptive feature of the survival of Stipagrostis pennata in desert systems. Although microorganisms play important ecological roles in promoting the nitrogen cycle of rhizosheath, the diversity and function of nitrogen-fixing microorganism communities have not been fully understood. Samples and methods :Therefore, the aim of this study is to explore the nitrogen fixation ability of rhizosheaths and the changes in abundance of nitrogen-fixing microorganisms at different growth periods of Stipagrostis pennata. We sequenced the nifH gene through sequencing to identify the structure and diversity of nitrogen-fixing microorganisms of Stipagrostis pennata at different growth periods of rhizosheaths. Results: A total of 1256 operational taxonomic unit (OTUs) were identified by nifH sequence and distributed in different growth periods. There were 5 OTUs distributed in each sample at the same time, and the abundance and diversity of microorganisms in fruit period were much higher than those in other periods. Mainly 4 phyla were involved, among which Proteobacteria was the most abundant in all groups. Conclusions: In general, this study investigated the abundance and characteristics of nitrogen-fixing microorganisms of rhizosheaths in different growth periods of Stipagrostis pennata. It also may elucidate indicate that the structure of nitrogen-fixing microorganisms of rhizosheaths in different growth periods of Stipagrostis pennata had changed.

Explore the Structural Changes of Nitrogen-fixing Microorganisms of Rhizosheath During the Growth of Stipagrostis Pennata in the Desert

Background: Rhizosheath is an adaptive feature of the survival of Stipagrostis pennata in desert systems. Although microorganisms play important ecological roles in promoting the nitrogen cycle of rhizosheath, the diversity and function of nitrogen-fixing microorganism communities have not been fully understood. Therefore, the aim of this study is to explore the nitrogen fixation ability of rhizosheaths and the changes of nitrogen-fixing microorganisms at different growth periods of Stipagrostis pennata. We sequenced the nifH gene through sequencing nifH gene to identify the structure and diversity of nitrogen-fixing microorganisms of Stipagrostis pennata at different growth periods of rhizosheaths.Results: A total of 1256 OTUs were identified by nifH sequence and distributed in different growth periods. There were 5 OTUs distributed in each sample at the same time, and the abundance and diversity of microorganisms in fruit period were much higher than those in other periods. Mainly 4 phyla were involved, among which Proteobacteria was the most abundant in all groups.Conclusions: In general, this study investigated the abundance and characteristics of nitrogen-fixing microorganisms of rhizosheaths in different growth periods of Stipagrostis pennata. It also elucidated the regulatory changes of the structure of nitrogen-fixing microorganisms of rhizosheaths in different growth periods of Stipagrostis pennata.