Pengembangan Populasi Mutan Penanda Aktivasi: I. Transformasi Padi Japonica Tropis Lokal Sulawesi cv. Asemandi dengan bantuan Agrobacterium tumefaciens

<p>The rice transformation technology<br />is not only provides valuable methods for the introduction<br />of useful genes into rice plant to improve important<br />agronomic traits, but also helps in studying gene function<br />and regulation based on rice genome sequence information.<br />Knockout of genes by insertional mutagenesis is a straightforward<br />method to identify gene functions. One of the<br />methods to develop rice mutants is through genetic transformation<br />mediated by Agrobacterium using activation<br />tagging by Ac-Ds system. A study was done with an objective<br />to obtain mutant rice of local tropical japonica cv. Asemandi<br />through genetic trans-formation mediated by Agrobacterium<br />tumefaciens. The transformation was conducted using<br />Agrobacterium vector with the strain of Agl-1 containing<br />activation tag construct. The result of experiment showed<br />that it has been obtained 17 independent line (304 plants)<br />transgenic Asemandi containing activation tag construct.<br />These starter lines will be used as materials to develop<br />several generations of stabil rice mutant through selfing.</p>