Diamond Raman Laser and Yb Fiber Amplifier for In Vivo Multiphoton Fluorescence Microscopy

Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, 1250 nm) for multiphoton excitation of red-shifted fluorophores. These custom excitation sources are both simple to construct and cost-efficient in comparison to similar custom and commercial alternatives. Furthermore, they operate at a repetition rate (80 MHz) that allows fast image acquisition using resonant scanners. We demonstrate our system's compatibility with fast resonant scanning, the ability to acquire neuronal images, and the capability to image vasculature at deep locations (>1 mm) within the mouse cerebral cortex.

Sonography of the Sacrotuberous Ligaments: Landmarks for Obtaining an Optimal Longitudinal Section

Objective: to develop a landmark suitable for fast image acquisition of the sacroiliac ligament in the longitudinal section. Material and methods. The position of the longitudinal axis of the sacro-ligamentous ligaments relative to the line of the spinous processes on the sonograms of 36 patients (the age range was 27-80) and 33 cadavers (the age range was 50-75) has been determined. Results. The obtained data allow to trace the image of the sacrotuberous ligament in the longitudinal section quickly (using the ischial tuberosity as a landmark and orienting the scanning plane of the sensor at an angle of 19-25° according to the line of the spinous processes).