rump cycle
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2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Philipp Keller ◽  
Elad Noor ◽  
Fabian Meyer ◽  
Michael A. Reiter ◽  
Stanislav Anastassov ◽  
...  

Abstract Methanol is a biotechnologically promising substitute for food and feed substrates since it can be produced renewably from electricity, water and CO2. Although progress has been made towards establishing Escherichia coli as a platform organism for methanol conversion via the energy efficient ribulose monophosphate (RuMP) cycle, engineering strains that rely solely on methanol as a carbon source remains challenging. Here, we apply flux balance analysis to comprehensively identify methanol-dependent strains with high potential for adaptive laboratory evolution. We further investigate two out of 1200 candidate strains, one with a deletion of fructose-1,6-bisphosphatase (fbp) and another with triosephosphate isomerase (tpiA) deleted. In contrast to previous reported methanol-dependent strains, both feature a complete RuMP cycle and incorporate methanol to a high degree, with up to 31 and 99% fractional incorporation into RuMP cycle metabolites. These strains represent ideal starting points for evolution towards a fully methylotrophic lifestyle.



1992 ◽  
Vol 62 (3) ◽  
pp. 201-207 ◽  
Author(s):  
Margaret M. Attwood ◽  
Nico Arfman ◽  
Ruud A. Weusthuis ◽  
Lubbert Dijkhuizen


1982 ◽  
Vol 14 (4) ◽  
pp. 257-261 ◽  
Author(s):  
P.R. Levering ◽  
L. Dijkhuizen ◽  
W. Harder
Keyword(s):  


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