Tissue Engineering - Collagen Matrix Based Flaps - a Simple Construct. Experimental and Theoretical Aspects

In our study we demonstrate the possibility to create a free flap using only a collagen matrix and a vascular pedicle. The processes of cell migration and differentiation, endogenous collagen synthesis, vascular network development, all rely on the arteriovenous loop introduced in the scaffold with no participation of surrounding vascularity. The flap has the capability to revascularize a full thickness skin graft, and also to establish vascular connections with adjacent vascular territories. Also, a fractal mathematical model was built based on spontaneous breaking symmetry to explain the behaviors of biostructures mentioned above. Keywords: tissue engineering, collagen matrix, neovascularisation, free flap