single filing
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2009 ◽  
Vol 28 (S12) ◽  
pp. 1-13 ◽  
Author(s):  
Dan W. Urry ◽  
Tina L. Trapane ◽  
C. M. Venkatachalam ◽  
Kari U. Prasad


2008 ◽  
Vol 95 (2) ◽  
pp. 609-619 ◽  
Author(s):  
Dirk Gillespie ◽  
Dezső Boda ◽  
Yan He ◽  
Pavel Apel ◽  
Zuzanna S. Siwy


1986 ◽  
Vol 88 (2) ◽  
pp. 167-194 ◽  
Author(s):  
L Simchowitz ◽  
P De Weer

Chloride content and fluxes were measured in isolated resting human peripheral polymorphonuclear leukocytes. The intracellular Cl concentration of cells kept at 37 degrees C in 148 mM Cl media was approximately 80 meq/liter cell water, fourfold higher than expected for passive distribution at the cell's estimated membrane potential (approximately -53 mV). All intracellular Cl was rapidly exchangeable with external 36Cl. Cells lost Cl exponentially into Cl-free media, and reaccumulated it when Cl was restored to the bath; this reuptake was dependent on metabolism. One-way 36Cl fluxes in steady state cells were approximately 1.4 meq/liter X min. The bulk (approximately 70%) of these represented electrically silent Cl/Cl exchange mediated by a carrier insensitive to disulfonic stilbenes but blocked by the anion carrier inhibitor alpha-cyano-4-hydroxycinnamate (CHC). The remaining fluxes were characterized in some detail. About 20% of 36Cl influx behaved as active transport: it moved thermodynamically uphill and was absent in cells treated with 2-deoxy-D-glucose, displayed Michaelis-Menten kinetics with Km(Cl) congruent to 5 mM, Vmax congruent to 0.25 meq/liter X min, and was inhibited by CHC (Ki congruent to 1.7 mM), ethacrynate (Ki congruent to 50 microM), and furosemide (Ki congruent to 50 microM). About 30% of Cl efflux and approximately 8% of Cl influx behaved as electrodiffusion through a low-permeability pathway (PCl congruent to 4 X 10(-9) cm/s; gCl congruent to 1 microsecond/cm2; PK/PNa/PCl congruent to to 10:1:1); these fluxes were linear with concentration and strongly voltage sensitive. The putative Cl channel does not appear to be voltage gated, and gives evidence of single filing.



1983 ◽  
Vol 71 (1-2) ◽  
pp. 61-78 ◽  
Author(s):  
John Sandblom ◽  
George Eisenman ◽  
Jarl Hägglund
Keyword(s):  


Author(s):  
George Eisenman ◽  
John Sandblom ◽  
Jarl Hagglund


1980 ◽  
Vol 339 (1 Growth Regula) ◽  
pp. 8-20 ◽  
Author(s):  
G. Eisenman ◽  
B. Enos ◽  
J. Hägglund ◽  
J. Sandblom
Keyword(s):  




Cancer ◽  
1967 ◽  
Vol 20 (3) ◽  
pp. 363-370 ◽  
Author(s):  
Gerhard O. Richter ◽  
Malcolm B. Dockerty ◽  
O. Theron Clagett


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