Direct recovery of glutathioneS-transferase by expanded bed adsorption: Anion exchange as an alternative to metal affinity fusions

R. H. Clemmitt; H. A. Chase

doi:10.1002/bit.10167

Direct recovery of glutathioneS-transferase by expanded bed adsorption: Anion exchange as an alternative to metal affinity fusions

Biotechnology and Bioengineering ◽

10.1002/bit.10167 ◽

2002 ◽

Vol 77 (7) ◽

pp. 776-785 ◽

Cited By ~ 5

Author(s):

R. H. Clemmitt ◽

H. A. Chase

Keyword(s):

Anion Exchange ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Metal Affinity

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Evaluation of three expanded bed adsorption anion exchange matrices with the aid of recombinant enhanced green fluorescent protein overexpressed in Escherichia coli

Journal of Chromatography B ◽

10.1016/j.jchromb.2004.02.009 ◽

2004 ◽

Vol 808 (1) ◽

pp. 91-97 ◽

Cited By ~ 11

Author(s):

C Cabanne ◽

A.M Noubhani ◽

W Dieryck ◽

A Hocquellet ◽

X Santarelli

Keyword(s):

Escherichia Coli ◽

Green Fluorescent Protein ◽

Anion Exchange ◽

Enhanced Green Fluorescent Protein ◽

Fluorescent Protein ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Green Fluorescent

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The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration

Biotechnology and Bioprocess Engineering ◽

10.1007/bf02932316 ◽

2006 ◽

Vol 11 (5) ◽

pp. 466-469 ◽

Cited By ~ 2

Author(s):

Yen Mei Chow ◽

Beng Ti Tey ◽

Mohd Nordin Ibrahim ◽

Arbakariya Ariff ◽

Tau Chuan Ling

Keyword(s):

Anion Exchange ◽

Biomass Concentration ◽

Adsorption Chromatography ◽

Expanded Bed Adsorption ◽

High Biomass ◽

Expanded Bed ◽

High Biomass Concentration

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Purification of His-tagged hepatitis B core antigen from unclarified bacterial homogenate using immobilized metal affinity-expanded bed adsorption chromatography

Journal of Chromatography A ◽

10.1016/j.chroma.2010.03.012 ◽

2010 ◽

Vol 1217 (21) ◽

pp. 3473-3480 ◽

Cited By ~ 26

Author(s):

Wei Boon Yap ◽

Beng Ti Tey ◽

Noorjahan Banu Mohamed Alitheen ◽

Wen Siang Tan

Keyword(s):

Hepatitis B ◽

Adsorption Chromatography ◽

Expanded Bed Adsorption ◽

Core Antigen ◽

Expanded Bed ◽

Metal Affinity ◽

Hepatitis B Core Antigen

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Expanded-bed adsorption immobilized-metal affinity chromatography

Nature Protocols ◽

10.1038/nprot.2006.127 ◽

2006 ◽

Vol 1 (3) ◽

pp. 1213-1222 ◽

Cited By ~ 18

Author(s):

Berend Tolner ◽

Lisa Smith ◽

Richard H J Begent ◽

Kerry A Chester

Keyword(s):

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Metal Affinity

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Facilitated downstream processing of a histidine-tagged protein from unclarifiedE. coli homogenates using immobilized metal affinity expanded-bed adsorption

Biotechnology and Bioengineering ◽

10.1002/(sici)1097-0290(20000120)67:2<206::aid-bit10>3.0.co;2-x ◽

2000 ◽

Vol 67 (2) ◽

pp. 206-216 ◽

Cited By ~ 76

Author(s):

R. H. Clemmitt ◽

H. A. Chase

Keyword(s):

Downstream Processing ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Metal Affinity

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Immobilised metal affinity chromatography of β-galactosidase from unclarified Escherichia coli homogenates using expanded bed adsorption

Journal of Chromatography A ◽

10.1016/s0021-9673(00)00087-x ◽

2000 ◽

Vol 874 (1) ◽

pp. 27-43 ◽

Cited By ~ 40

Author(s):

R.H Clemmitt ◽

H.A Chase

Keyword(s):

Escherichia Coli ◽

Affinity Chromatography ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Metal Affinity ◽

Immobilised Metal Affinity Chromatography

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On-line purification of His-tag enhanced green fluorescent protein taken directly from a bioreactor by continuous ultrasonic homogenization coupled with immobilized metal affinity expanded bed adsorption

Journal of Chromatography A ◽

10.1016/s0021-9673(02)00794-x ◽

2002 ◽

Vol 968 (1-2) ◽

pp. 113-120 ◽

Cited By ~ 16

Author(s):

A.M Noubhani ◽

W Dieryck ◽

S Chevalier ◽

X Santarelli

Keyword(s):

Green Fluorescent Protein ◽

Enhanced Green Fluorescent Protein ◽

Fluorescent Protein ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Metal Affinity ◽

His Tag ◽

On Line ◽

Green Fluorescent

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Modeling and simulation of breakthrough curves of recombinant 503 antigen using immobilized metal affinity expanded bed adsorption chromatography

Separation and Purification Technology ◽

10.1016/j.seppur.2016.03.019 ◽

2016 ◽

Vol 164 ◽

pp. 34-40 ◽

Cited By ~ 9

Author(s):

Francisco Caninde de Sousa Junior ◽

Carlos Eduardo de Araújo Padilha ◽

Abimaelle Silva Chibério ◽

Vitor Troccoli Ribeiro ◽

Daniella Regina Arantes Martins ◽

...

Keyword(s):

Modeling And Simulation ◽

Breakthrough Curves ◽

Adsorption Chromatography ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Metal Affinity

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LdARL-1 His-tagged recombinant protein: purification by immobilized metal affinity expanded bed adsorption

Journal of Chromatography B ◽

10.1016/j.jchromb.2004.10.022 ◽

2005 ◽

Vol 818 (1) ◽

pp. 19-22 ◽

Cited By ~ 9

Author(s):

Annelise Sahin ◽

Emmanuel Tetaud ◽

Gilles Merlin ◽

Xavier Santarelli

Keyword(s):

Recombinant Protein ◽

Protein Purification ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Recombinant Protein Purification ◽

Metal Affinity

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Purification of recombinant histidine-tag streptolysin O using immobilized metal affinity expanded bed adsorption (IMA-EBA)

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2006.02.005 ◽

2006 ◽

Vol 38 (2) ◽

pp. 134-139 ◽

Cited By ~ 8

Author(s):

Sandra Camprubí ◽

Marc Bruguera ◽

Francesca Canalias

Keyword(s):

Expanded Bed Adsorption ◽

Expanded Bed ◽

Metal Affinity ◽

Streptolysin O ◽

Histidine Tag

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