The influence of high substrate concentrations on microbial kinetics

1970 ◽  
Vol 12 (5) ◽  
pp. 679-712 ◽  
Author(s):  
Victor H. Edwards
Keyword(s):  
High Substrate ◽  
Microbial Kinetics ◽  
Substrate Concentrations

Production of cephalexin in organic medium at high substrate concentrations with CLEA of penicillin acylase and PGA-450

2007 ◽  
Vol 40 (2) ◽  
pp. 195-203 ◽  
Author(s):  
A. Illanes ◽  
L. Wilson ◽  
C. Altamirano ◽  
Z. Cabrera ◽  
L. Alvarez ◽  
...  
Keyword(s):  
Penicillin Acylase ◽  
Organic Medium ◽  
High Substrate ◽  
Substrate Concentrations

Assessment of pretreatment conditions to obtain fast complete hydrolysis on high substrate concentrations

1989 ◽  
Vol 20-21 (1) ◽  
pp. 29-44 ◽  
Author(s):  
W. Schwald ◽  
C. Breuil ◽  
H. H. Brownell ◽  
M. Chan ◽  
J. M. Saddler
Keyword(s):  
High Substrate ◽  
Complete Hydrolysis ◽  
Pretreatment Conditions ◽  
Substrate Concentrations

scholarly journals Continuous enzymatic stirred tank reactor cascade with unconventional medium yielding high concentrations of (S)-2-hydroxyphenyl propanone and its derivatives

2021 ◽  
Author(s):  
Reinhard Oeggl ◽  
Juliane Diedrich ◽  
Eric von Lieres ◽  
Dörte Rother
Keyword(s):  
Green Chemistry ◽  
Flow Chemistry ◽  
Stirred Tank ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
High Substrate ◽  
High Concentrations ◽  
Substrate Concentrations

The implementation of biocatalysis in flow chemistry offers synergistic synthesis advantages in line with green chemistry principles. Yet, the conversion of high substrate concentrations is most often hindered by substrate...

Kinetic laws for solid-supported enzymes

1970 ◽  
Vol 48 (10) ◽  
pp. 1498-1504 ◽  
Author(s):  
P. V. Sundaram ◽  
A. Tweedale ◽  
K. J. Laidler
Keyword(s):  
Substrate Concentration ◽  
Free Solution ◽  
Kinetic Behavior ◽  
Michaelis Constant ◽  
Solid Supports ◽  
High Substrate ◽  
Substrate Concentrations

Enzymes behave differently when attached to solid supports for four main reasons: (1) their conformations when they are supported may differ from those in free solution, (2) they act upon substrates in a different environment, (3) there will be partitioning of substrate between the support and the free solution, and (4) there will be effects due to diffusion of the substrate in the support. The present paper examines effects (3) and (4) and shows how rates will vary with substrate concentration. If factors (1) and (2) do not enter, rates in the limit of high substrate concentrations will be the same for the supported enzyme as in free solution. At low substrate concentrations, rates will be less for the supported enzyme if the substrate is less soluble in the support than in free solution, and the apparent Michaelis constant, Km(app.), will be greater; conversely, for higher solubility in the support, rates will be greater and Km(app.) smaller. Effect (4) leads to lower rates and higher Km(app.) values, except in the limit of high substrate concentrations. At a sufficiently low thickness of the support, depending upon the activity of the enzyme, the kinetic behavior becomes identical with that in free solution.

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