Liquid chromatography-tandem mass spectrometric assay for the determination of vaccarin in rat plasma: application to a pharmacokinetic study

2014 ◽  
Vol 28 (12) ◽  
pp. 1789-1794 ◽  
Author(s):  
Guilin Yang ◽  
Nan Zhang ◽  
Tiance Wang ◽  
Shudong Zhang ◽  
Rihao Xu ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Liquid Chromatography Tandem Mass ◽  
Tandem Mass Spectrometric

scholarly journals A Simple and Sensitive Liquid Chromatography with Tandem Mass Spectrometric Method for the Simultaneous Determination of Anthraquinone Glycosides and Their Aglycones in Rat Plasma: Application to a Pharmacokinetic Study of Rumex acetosa Extract

Pharmaceutics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 100 ◽  
Author(s):  
Hossain Ullah ◽  
Junhyeong Kim ◽  
Naveed Rehman ◽  
Hye-Jin Kim ◽  
Mi-Jeong Ahn ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Rumex Acetosa ◽  
Anthraquinone Glycosides ◽  
Tandem Mass Spectrometric

Rumex acetosa (R. acetosa) has been used in folk remedies for gastrointestinal disorders and cutaneous diseases. Rumex species, in particular, contain abundant anthraquinones. Anthraquinone glycosides and aglycones show different bioactive effects. However, information on the pharmacokinetics of anthraquinone glycosides is limited, and methods to quantify anthraquinone glycosides in plasma are rarely available. A simple and sensitive liquid chromatography-tandem mass spectrometric bioanalytical method for the simultaneous determination of both anthraquinone glycosides and their aglycones, including emodin, emodin-8-O-β-d-glucoside, chrysophanol, chrysophanol-8-O-β-d-glucoside, physcion, and physcion-8-O-β-d-glucoside , in a low volume of rat plasma (20 µL) was established. A simple and rapid sample preparation was employed using methanol as a precipitating agent with appropriate sensitivity. Chromatographic separation was performed on HPLC by using a biphenyl column with a gradient elution using 2 mM ammonium formate (pH 6) in water and 2 mM ammonium formate (pH 6) in methanol within a run time of 13 min. The anthraquinones were detected on triple-quadrupole mass spectrometer in negative ionization mode using multiple-reaction monitoring. The method was validated in terms of selectivity, linearity, accuracy, precision, recovery, and stability. The values of the lower limit of quantitation of anthraquinones were 1–20 ng/mL. The intra-batch and inter-batch accuracies were 96.7–111.9% and the precision was within the acceptable limits. The method was applied to a pharmacokinetic study after oral administration of R. acetosa 70% ethanol extract to rats at a dose of 2 g/kg.

Liquid chromatography-tandem mass spectrometric assay for determination of unstable arecoline in rat plasma and its application

2017 ◽  
Vol 1070 ◽  
pp. 112-116 ◽  
Author(s):  
Hong Pan ◽  
Linyan Huang ◽  
Yi Li ◽  
Xumei Zhou ◽  
Yuanfu Lu ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Rat Plasma ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Liquid Chromatography Tandem Mass ◽  
Tandem Mass Spectrometric

Liquid chromatography-tandem mass spectrometric assay for the determination of tetrabenazine and its active metabolites in human plasma: a pharmacokinetic study

2013 ◽  
Vol 27 (6) ◽  
pp. 792-801 ◽  
Author(s):  
Venkata Ramu Derangula ◽  
Nageswara Rao Pilli ◽  
Siva Kumar Nadavala ◽  
Vinayender Adireddy ◽  
Jaswanth Kumar Inamadugu ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Human Plasma ◽  
Pharmacokinetic Study ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Active Metabolites ◽  
Liquid Chromatography Tandem Mass ◽  
Tandem Mass Spectrometric
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