The problem of environmental pollution day by day becomes more worrisome,
primarily due to the large amounts of wastewater contaminated with various
harmful organic compounds, discharged into the environment untreated or
partially clean. Feasibility of use of horseradish peroxidase (Amoracia
rusticana) in the synthetic dyes decolorization was approved by many
researchers. Among a number of supports used for the immobilization, it was
found that natural clay, kaolin has excellent features which are a
precondition for obtaining biocatalysts with the excellent performances. For
this reason, a horseradish peroxidase was immobilized onto kaolin using
glutaraldehyde as a cross-linking agent. Obtained biocatalyst was applied in
the decolorization of anthraquinone dye C. I. Acid Violet 109. Under
determined optimal conditions (pH 4.0, hydrogen peroxide concentration 0.6
mM, dye concentration 30 mg L-1, temperature 24?C) around 76 % of dye
decolorization was achieved. Reusability study showed that resulting
biocatalyst was possible to apply four times in the desired reaction with
relatively high decolorization percentage.
Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye
