Synthesis of Chimeric Oligonucleotides Having Modified Internucleotide Linkages via an Automated H-Phosphonate/Phosphoramidite Approach

2018 ◽  
Vol 73 (1) ◽  
pp. e53 ◽  
Author(s):  
Danielle Vlaho ◽  
Masad J. Damha
Keyword(s):  
Chimeric Oligonucleotides

scholarly journals Efficient Inhibition of SARS‐CoV‐2 Using Chimeric oligonucleotides through RNase L Activation

2021 ◽  
Author(s):  
Xiaoxuan Su ◽  
Wenxiao Ma ◽  
Di Feng ◽  
Boyang Cheng ◽  
Qian Wang ◽  
...  
Keyword(s):  
Rnase L ◽  
Chimeric Oligonucleotides

Targeted Gene Repair in Mammalian Cells Using Chimeric Oligonucleotides

2000 ◽  
pp. 23-31 ◽  
Author(s):  
Eric B. Kmiec ◽  
Sarah Ye ◽  
Lan Peng
Keyword(s):  
Mammalian Cells ◽  
Gene Repair ◽  
Targeted Gene Repair ◽  
Chimeric Oligonucleotides

scholarly journals Efficient Inhibition of SARS-CoV-2 Using Chimeric Antisense Oligonucleotides through RNase L Activation

2021 ◽  
Author(s):  
Xiaoxuan Su ◽  
Wenxiao Ma ◽  
Boyang Cheng ◽  
Qian Wang ◽  
Zefeng Guo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Antisense Oligonucleotides ◽  
Target Recognition ◽  
Vero Cells ◽  
Rnase L ◽  
Rna Targets ◽  
Infection Models ◽  
Antisense Sequence ◽  
Innate Antiviral Response ◽  
Chimeric Oligonucleotides

AbstractThere is an urgent need for effective antiviral drugs to alleviate the current COVID-19 pandemic. Here, we rationally designed and developed chimeric antisense oligonucleotides to degrade envelope and spike RNAs of SARS-CoV-2. Each oligonucleotide comprises a 3’ antisense sequence for target recognition and a 5’-phosphorylated 2’-5’ poly(A)4 for guided ribonuclease L (RNase L) activation. Since RNase L can potently cleave single strand RNA during innate antiviral response, the improved degradation efficiency of chimeric oligonucleotides was twice as much as classic antisense oligonucleotides in Vero cells, for both SARS-CoV-2 RNA targets. In pseudovirus infection models, one of chimeric oligonucleotides targeting spike RNA achieved potent and broad-spectrum inhibition of both SARS-CoV-2 and its recently reported N501Y and/or ΔH69/ΔV70 mutants. These results showed that the constructed chimeric oligonucleotides could efficiently degrade pathogenic RNA of SARS-CoV-2 facilitated by immune activation, showing promising potentials as antiviral nucleic acid drugs for COVID-19.

scholarly journals Solid-Phase Synthesis of Phosphorothioate/Phosphonothioate and Phosphoramidate/Phosphonamidate Oligonucleotides

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1872 ◽  
Author(s):  
Ondřej Kostov ◽  
Radek Liboska ◽  
Ondřej Páv ◽  
Pavel Novák ◽  
Ivan Rosenberg
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
The Novel ◽  
Phase Synthesis ◽  
Chimeric Oligonucleotides ◽  
Modified Oligonucleotide

We have developed a robust solid-phase protocol which allowed the synthesis of chimeric oligonucleotides modified with phosphodiester and O-methylphosphonate linkages as well as their P-S and P-N variants. The novel O-methylphosphonate-derived modifications were obtained by oxidation, sulfurization, and amidation of the O-methyl-(H)-phosphinate internucleotide linkage introduced into the oligonucleotide chain by H-phosphonate chemistry using nucleoside-O-methyl-(H)-phosphinates as monomers. The H-phosphonate coupling followed by oxidation after each cycle enabled us to successfully combine H-phosphonate and phosphoramidite chemistries to synthesize diversely modified oligonucleotide strands.

A Plausible Mechanism for Gene Correction by Chimeric Oligonucleotides†

Biochemistry ◽  
2000 ◽  
Vol 39 (19) ◽  
pp. 5808-5816 ◽  
Author(s):  
Howard B. Gamper, ◽  
Allyson Cole-Strauss ◽  
Richard Metz ◽  
Hetal Parekh ◽  
Ramesh Kumar ◽  
...  
Keyword(s):  
Gene Correction ◽  
Plausible Mechanism ◽  
Chimeric Oligonucleotides

scholarly journals Stereocontrolled synthesis of oligonucleoside phosphorothioates and PO/PS-chimeric oligonucleotides by using oxazaphospholidine derivatives

2008 ◽  
Vol 52 (1) ◽  
pp. 335-336 ◽  
Author(s):  
N. Oka ◽  
M. Yamamoto ◽  
T. Sato ◽  
T. Wada
Keyword(s):  
Stereocontrolled Synthesis ◽  
Chimeric Oligonucleotides

Synthesis and Properties of 2'-O-Methylribonucleotide Methylphosphonate Containing Chimeric Oligonucleotides

1995 ◽  
Vol 14 (3) ◽  
pp. 1031-1035 ◽  
Author(s):  
Ekambar Kandimalla ◽  
Jamal Temsamani ◽  
Sudhir Agrawal
Keyword(s):  
Chimeric Oligonucleotides

α−β Chimeric Oligonucleotides Form a New Stable “Snail-like” Structure

1996 ◽  
Vol 118 (9) ◽  
pp. 2126-2130 ◽  
Author(s):  
M. B. Gottikh ◽  
O. A. Fedorova ◽  
M.-V. Baud-Demattei ◽  
S. Giorgi-Renault ◽  
J.-R. Bertrand ◽  
...  
Keyword(s):  
Chimeric Oligonucleotides

Abstract 3870: Improved PCR amplification of DNA biomarkers with the use of chimeric oligonucleotides

2011 ◽  
Author(s):  
John M. Millholland ◽  
Sheryl G. Patel ◽  
Anthony P. Shuber
Keyword(s):  
Pcr Amplification ◽  
Chimeric Oligonucleotides
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