Culturing Rat Whole Ovary for UV Filter Benzophenone‐3 Treatment

2019 ◽  
Vol 82 (1) ◽  
Author(s):  
Clarisa Guillermina Santamaría ◽  
Julián Elías Abud ◽  
Enrique Hugo Luque ◽  
Laura Kass ◽  
Horacio Adolfo Rodríguez
Keyword(s):  

2019 ◽  
Vol 303 ◽  
pp. 48-54 ◽  
Author(s):  
C.G. Santamaría ◽  
J.E. Abud ◽  
M.M. Porporato ◽  
N. Meyer ◽  
A.C. Zenclussen ◽  
...  
Keyword(s):  


1998 ◽  
Vol 26 (5) ◽  
pp. 679-708 ◽  
Author(s):  
Horst Spielmann ◽  
Michael Balls ◽  
Jack Dupuis ◽  
Wolfgang J. W. Pape ◽  
Odile de Silva ◽  
...  

In 1996, the Scientific Committee on Cosmetology of DGXXIV of the European Commission asked the European Centre for the Validation of Alternative Methods to test eight UV filter chemicals from the 1995 edition of Annex VII of Directive 76/768/EEC in a blind trial in the in vitro 3T3 cell neutral red uptake phototoxicity (3T3 NRU PT) test, which had been scientifically validated between 1992 and 1996. Since all the UV filter chemicals on the positive list of EU Directive 76/768/EEC have been shown not to be phototoxic in vivo in humans under use conditions, only negative effects would be expected in the 3T3 NRU PT test. To balance the number of positive and negative chemicals, ten phototoxic and ten non-phototoxic chemicals were tested under blind conditions in four laboratories. Moreover, to assess the optimum concentration range for testing, information was provided on appropriate solvents and on the solubility of the coded chemicals. In this study, the phototoxic potential of test chemicals was evaluated in a prediction model in which either the Photoirritation Factor (PIF) or the Mean Photo Effect (MPE) were determined. The results obtained with both PIF and MPE were highly reproducible in the four laboratories, and the correlation between in vitro and in vivo data was almost perfect. All the phototoxic test chemicals provided a positive result at concentrations of 1μg/ml, while nine of the ten non-phototoxic chemicals gave clear negative results, even at the highest test concentrations. One of the UV filter chemicals gave positive results in three of the four laboratories only at concentrations greater than 100μg/ml; the other laboratory correctly identified all 20 of the test chemicals. An analysis of the impact that exposure concentrations had on the performance of the test revealed that the optimum concentration range in the 3T3 NRU PT test for determining the phototoxic potential of chemicals is between 0.1μg/ml and 10μg/ml, and that false positive results can be obtained at concentrations greater than 100μg/ml. Therefore, the positive results obtained with some of the UV filter chemicals only at concentrations greater than 100μg/ml do not indicate a phototoxic potential in vivo. When this information was taken into account during calculation of the overall predictivity of the 3T3 NRU PT test in the present study, an almost perfect correlation of in vitro versus in vivo results was obtained (between 95% and 100%), when either PIF or MPE were used to predict the phototoxic potential. The management team and participants therefore conclude that the 3T3 NRU PT test is a valid test for correctly assessing the phototoxic potential of UV filter chemicals, if the defined concentration limits are taken into account.



Author(s):  
Jianmin Chen ◽  
Mengnan Ran ◽  
Meixia Wang ◽  
Xinying Liu ◽  
Siwan Liu ◽  
...  


FEBS Letters ◽  
2001 ◽  
Vol 509 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Lisa M. Taylor ◽  
J.Andrew Aquilina ◽  
Richard H. Willis ◽  
Joanne F. Jamie ◽  
Roger J.W. Truscott
Keyword(s):  


2013 ◽  
Vol 35 (3) ◽  
pp. 208-219 ◽  
Author(s):  
E. Gilbert ◽  
F. Pirot ◽  
V. Bertholle ◽  
L. Roussel ◽  
F. Falson ◽  
...  


2008 ◽  
Vol 86 (6) ◽  
pp. 479-483 ◽  
Author(s):  
E. Zampiga ◽  
G. Gaibani ◽  
D. Csermely

Previous studies revealed the importance of plumage brightness (considering only the visible spectral range) in female mate choice of common kestrels ( Falco tinnunculus L., 1758). Our study focuses on the effect of UV reflectance on female preference; we hypothesize that common kestrel females prefer males with UV-reflecting plumage and refuse, or are not attracted to, males whose plumage UV reflectance is prevented. We tested the preference of 20 captive females who were given a choice between two males, one behind a UV-transmitting (UV+) filter and another one behind a UV-blocking (UV–) filter. Female preference was measured as frequency and duration of visits to either male, specifically by sitting on the perch or hanging on to the partition wall. Females visited the male behind the UV+ filter more often than the male behind the UV– filter, indicating that females prefer males with UV-reflecting plumage than males without UV reflection. UV plumage and skin reflectance could provide females with information regarding current male conditions more so than other colours and other morphological parameters.



1958 ◽  
Vol 29 (8) ◽  
pp. 738-739 ◽  
Author(s):  
Robert H. McFarland ◽  
Richard A. Anderson ◽  
Mohammed Nasim ◽  
Donald G. McDonald
Keyword(s):  


FEBS Letters ◽  
1994 ◽  
Vol 348 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Roger J.W. Truscott ◽  
Andrew M. Wood ◽  
John A. Carver ◽  
Margaret M. Sheil ◽  
Glen M. Stutchbury ◽  
...  
Keyword(s):  


2021 ◽  
Vol 32 (19) ◽  
pp. 24011-24025
Author(s):  
M. Neetha Mehathaj ◽  
N. Padmanathan ◽  
E. Sivasenthil


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