Flow-cytometric quantification in human gliomas of alpha satellite DNA sequences specific for chromosome 7 using fluorescence in situ hybridization

A complete understanding of chromosomal disjunction during mitosis and meiosis in complex genomes such as the human genome awaits detailed characterization of both the molecular structure and genetic behavior of the centromeric regions of chromosomes. Such analyses in turn require knowledge of the organization and nature of DNA sequences associated with centromeres. The most prominent class of centromeric DNA sequences in the human genome is the alpha satellite family of tandemly repeated DNA, which is organized as distinct chromosomal subsets. Each subset is characterized by a particular multimeric higher-order repeat unit consisting of tandemly reiterated, diverged alpha satellite monomers of approximately 171 base pairs. The higher-order repeat units are themselves tandemly reiterated and represent the most recently amplified or fixed alphoid sequences. We present evidence that there are at least two independent domains of alpha satellite DNA on chromosome 7, each characterized by their own distinct higher-order repeat structure. We determined the complete nucleotide sequences of a 6-monomer higher-order repeat unit, which is present in approximately 500 copies per chromosome 7, as well as those of a less-abundant (approximately 10 copies) 16-monomer higher-order repeat unit. Sequence analysis indicated that these repeats are evolutionarily distinct. Genomic hybridization experiments established that each is maintained in relatively homogeneous tandem arrays with no detectable interspersion. We propose mechanisms by which multiple unrelated higher-order repeat domains may be formed and maintained within a single chromosomal subset.

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Determination of the breakpoints of 1;7 translocations in myelodysplastic syndrome by in situ hybridization using chromosome-specific α satellite DNA from human chromosomes 1 and 7

Cytogenetic and Genome Research ◽

10.1159/000132718 ◽

1989 ◽

Vol 50 (1) ◽

pp. 49-53 ◽

Cited By ~ 25

Author(s):

T. Alitalo ◽

H.F. Willard ◽

A. de la Chapelle

Keyword(s):

In Situ Hybridization ◽

Myelodysplastic Syndrome ◽

Satellite Dna ◽

Human Chromosomes ◽

Alpha Satellite ◽

Alpha Satellite Dna

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Genomic organization of alpha satellite DNA on human chromosome 7: evidence for two distinct alphoid domains on a single chromosome.

Molecular and Cellular Biology ◽

10.1128/mcb.7.1.349 ◽

1987 ◽

Vol 7 (1) ◽

pp. 349-356 ◽

Cited By ~ 153

Author(s):

J S Waye ◽

S B England ◽

H F Willard

Keyword(s):

Human Genome ◽

Dna Sequences ◽

Satellite Dna ◽

Repeat Unit ◽

Higher Order ◽

Chromosome 7 ◽

Alpha Satellite ◽

Single Chromosome ◽

Alpha Satellite Dna ◽

Repeat Structure

A complete understanding of chromosomal disjunction during mitosis and meiosis in complex genomes such as the human genome awaits detailed characterization of both the molecular structure and genetic behavior of the centromeric regions of chromosomes. Such analyses in turn require knowledge of the organization and nature of DNA sequences associated with centromeres. The most prominent class of centromeric DNA sequences in the human genome is the alpha satellite family of tandemly repeated DNA, which is organized as distinct chromosomal subsets. Each subset is characterized by a particular multimeric higher-order repeat unit consisting of tandemly reiterated, diverged alpha satellite monomers of approximately 171 base pairs. The higher-order repeat units are themselves tandemly reiterated and represent the most recently amplified or fixed alphoid sequences. We present evidence that there are at least two independent domains of alpha satellite DNA on chromosome 7, each characterized by their own distinct higher-order repeat structure. We determined the complete nucleotide sequences of a 6-monomer higher-order repeat unit, which is present in approximately 500 copies per chromosome 7, as well as those of a less-abundant (approximately 10 copies) 16-monomer higher-order repeat unit. Sequence analysis indicated that these repeats are evolutionarily distinct. Genomic hybridization experiments established that each is maintained in relatively homogeneous tandem arrays with no detectable interspersion. We propose mechanisms by which multiple unrelated higher-order repeat domains may be formed and maintained within a single chromosomal subset.

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Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences

Plant Cell Reports ◽

10.1007/s00299-011-1086-y ◽

2011 ◽

Vol 30 (9) ◽

pp. 1779-1786 ◽

Cited By ~ 5

Author(s):

Kun Yang ◽

Hecui Zhang ◽

Richard Converse ◽

Yong Wang ◽

Xiaoying Rong ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Repetitive Dna ◽

Dna Sequences ◽

Single Copy ◽

Repetitive Dna Sequences

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The numerical aberrations of chromosome 7 detected by fluorescence in situ hybridization in human breast cancers

Pathology International ◽

10.1111/j.1440-1827.1993.tb02559.x ◽

1993 ◽

Vol 43 (12) ◽

pp. 730-735

Author(s):

Naoki Yoshimi ◽

Chiken Shibuya ◽

Yukio Morishita ◽

Takuji Tanaka ◽

Hideki Mori

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Chromosome 7 ◽

Breast Cancers ◽

Human Breast ◽

Numerical Aberrations

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Identification of the entire chromosome complement of bread wheat by two-colour FISH

Genome ◽

10.1139/g97-077 ◽

1997 ◽

Vol 40 (5) ◽

pp. 589-593 ◽

Cited By ~ 125

Author(s):

C. Pedersen ◽

P. Langridge

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Dna Sequences ◽

Banding Pattern ◽

Aegilops Tauschii ◽

Chromosome Complement ◽

Chromosome Identification ◽

Satellite Sequence ◽

Entire Chromosome

Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.Key words: Triticum aestivum, chromosome identification, fluorescence in situ hybridization, repetitive DNA sequences.

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