Molecular analysis of the murine lupus-associated anti-self response: involvement of a large number of heavy and light chain variable region genes

1987 ◽  
Vol 17 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Reinhard Kofler ◽  
Daniel J. Noonan ◽  
Robert Strohal ◽  
Robert S. Balderas ◽  
Niels P. H. Moller ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Light Chain ◽  
Variable Region ◽  
Murine Lupus ◽  
Variable Region Genes

Primary Structure and Functional Expression of Heavy- and Light-Chain Variable Region Genes of a Monoclonal Antibody Specific for Human Fibrin

Hybridoma ◽  
1997 ◽  
Vol 16 (3) ◽  
pp. 235-241 ◽  
Author(s):  
ZENGXUAN SONG ◽  
YINGLIN CAI ◽  
DANYING SONG ◽  
JING XU ◽  
HONGWEI YUAN ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Primary Structure ◽  
Variable Region ◽  
Functional Expression ◽  
Variable Region Genes

scholarly journals Genetic elements used for a murine lupus anti-DNA autoantibody are closely related to those for antibodies to exogenous antigens.

1985 ◽  
Vol 161 (4) ◽  
pp. 805-815 ◽  
Author(s):  
R Kofler ◽  
D J Noonan ◽  
D E Levy ◽  
M C Wilson ◽  
N P Møller ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Light Chain ◽  
Variable Region ◽  
Leader Peptide ◽  
Murine Lupus ◽  
Heavy Chain Variable Region ◽  
Variable Gene ◽  
Vh Gene ◽  
Gene Origin ◽  
Common Variable

The mRNAs encoding heavy and light chains of a hybridoma-derived monoclonal IgM kappa anti-DNA autoantibody from lupus-prone MRL/Mp-lpr/lpr mice (Ighj) have been transcribed into cDNA copies and molecularly cloned, and their complete nucleotide sequences have been determined. The mRNA for the heavy chain variable region, including leader peptide and 5' untranslated region, is transcribed from a heavy chain variable region (VH) gene closely related (and possibly allelic) to VH genes of the C57BL/6 (Ighb) nitrophenyl antibody family. The deduced amino acid sequence corresponding to the light chain variable region of this autoantibody shows extensive similarities with non-autoantibody molecules of the V kappa 1 group, suggesting a common variable gene origin. The joining segments, constant regions, and 3' untranslated regions of both the heavy and light chain mRNAs are nearly identical to corresponding sequences of non-autoantibodies from normal mice. Our findings suggest that this anti-DNA autoantibody originated from the same germline repertoire as antibodies to exogenous antigens.

Profile of lambda light chain variable region genes in graves' orbital tissue

1994 ◽  
Vol 31 (11) ◽  
pp. 793-802 ◽  
Author(s):  
Mark F. Prummel ◽  
Gregorio Chazenbalk ◽  
Juan Carlos Jaume ◽  
Basil Rapoport ◽  
Sandra M. McLachlan
Keyword(s):  
Light Chain ◽  
Variable Region ◽  
Lambda Light Chain ◽  
Orbital Tissue ◽  
Variable Region Genes
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