Cell kinetics in human breast cancer: Comparison between the prognostic value of the cytofluorimetric S-phase fraction and that of the antibodies to Ki-67 and PCNA antigens detected by immunocytochemistry

1994 ◽  
Vol 57 (6) ◽  
pp. 822-829 ◽  
Author(s):  
Giampietro Gasparini ◽  
Patrizia Boracchi ◽  
Paolo Verderio ◽  
Pierantonio Bevilacqua
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Prognostic Value ◽  
Cell Kinetics ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast ◽  
Ki 67

The Flow-Cytometric Analysis of DNA Content and S-Phase Fraction (SPF) of Human Breast Cancer

1989 ◽  
Vol 185 (5) ◽  
pp. 694-697 ◽  
Author(s):  
M. Eskelinen ◽  
S. Nordling ◽  
J. Puittinen ◽  
E. Pesonen ◽  
Y. Collan
Keyword(s):  
Breast Cancer ◽  
Dna Content ◽  
Human Breast Cancer ◽  
Flow Cytometric Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast ◽  
Flow Cytometric

scholarly journals Prognostic Value of Proliferation Markers: Immunohistochemical Ki-67 Expression and Cytometric S-Phase Fraction of Women with Breast Cancer in Libya

2012 ◽  
Vol 3 ◽  
pp. 421-431 ◽  
Author(s):  
Eramah Ermiah ◽  
Abdelbaset Buhmeida ◽  
Fathi Abdalla ◽  
Ben Romdhane Khaled ◽  
Nada Salem ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Value ◽  
S Phase ◽  
Phase Fraction ◽  
Ki 67 ◽  
Proliferation Markers

Lack of prognostic significance of DNA Ploidy and S phase fraction in human breast cancer

The Breast ◽  
1992 ◽  
Vol 1 (3) ◽  
pp. 148
Author(s):  
PD Stanton ◽  
SJ Oakes ◽  
GD Murray ◽  
TG Cooke
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Dna Ploidy ◽  
Prognostic Significance ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast

scholarly journals IGF-I in human breast cancer: low differentiation stage is associated with decreased IGF-I content

2002 ◽  
pp. 813-821 ◽  
Author(s):  
E Eppler ◽  
J Zapf ◽  
N Bailer ◽  
UG Falkmer ◽  
S Falkmer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epithelial Cells ◽  
Human Breast Cancer ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast ◽  
Morphological Criteria ◽  
Igf I ◽  
Wet Weight ◽  
Differentiation Stage

OBJECTIVE: Few investigations on the potential role of IGF-I in human breast cancer have used morphological criteria, and the data presented on the localisation of IGF-I are controversial. Moreover, little information exists on a potential correlation between local IGF-I and the grade of malignancy or prognostic factors. Therefore, we investigated the immunohistochemical localisation of IGF-I in specimens of human breast cancer tumours of the ductal type, graded as G1/G2 (well-/moderately differentiated, n=115) and G3 (poorly differentiated, n=28). METHODS: IGF-I immunoreactivity was quantified using a scaling from no (-) to numerous (+++) IGF-I-immunoreactive cells. From 29 of the G1/G2 and 17 of the G3 tumours IGF-I was also measured by RIA. Cytosolic oestrogen receptor (ER) and progesterone receptor (PR) levels, and S-phase fraction were established and related to the number of IGF-I-immunoreactive cells. RESULTS: IGF-I immunoreactivity occurred predominantly in ductal epithelial cells. Of G3 tumours, 57% exhibited IGF-I immunoreactivity as compared with 84% of G1/G2 tumours. Correspondingly, the amount of IGF-I measured by RIA was significantly lower in G3 tumours (6.9+/-0.9 ng/g wet weight) than in G1/G2 tumours (10.5+/-1.1 ng/g wet weight) (P=0.031). G1/G2 tumours exhibited a higher percentage of IGF-I-immunoreactive cells (16% -, 23% +, 41% ++, 20% +++) than G3 tumours (43% -, 37% +, 12% ++, 8% +++). When comparing the - with the +++ G1/G2 tumours, the frequency of IGF-I-immunoreactive cells was related significantly to the ER (P<0.016) and the PR (P<0.008) levels. In G1/G2 and G3 tumours, the ER and PR levels increased with the amount of IGF immunoreactivity while the S-phase fraction increased with decreasing IGF-I content. In 25% of the specimens, IGF-I immunoreactivity occurred in stromal cells, but there was no obvious difference between the different types of tumours. The survival of the G1/G2 tumour patients increased with increasing numbers of IGF-I-immunoreactive cells. CONCLUSIONS: It is concluded that IGF-I is associated with the more-differentiated type of epithelial cells and that increasing dedifferentiation goes along with decreased IGF-I content. Thus, the presence of IGF-I immunoreactivity in breast cancer epithelial cells indicates a lower degree of malignancy than the lack of IGF-I.

scholarly journals Image cytometric DNA analysis in human breast cancer analysis may add prognostic information in diploid cases with low S-Phase fraction by flow cytometry

Cytometry ◽  
1992 ◽  
Vol 13 (6) ◽  
pp. 577-585 ◽  
Author(s):  
Bo Baldetorp ◽  
Mårten Fernö ◽  
Anders Fallenius ◽  
Ghita Fallenius-Vecchi ◽  
Ingrid Idvall ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Human Breast Cancer ◽  
Dna Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast ◽  
Prognostic Information

Loss of Retinoblastoma Gene (RB1) Is Associated with Deletions at the 17p13.3 Chromosome and S-Phase Index in Human Breast Cancer

1996 ◽  
Vol 784 (1 Challenges an) ◽  
pp. 462-466
Author(s):  
TIZIANA VENESIO ◽  
AMELIA BERNARDI ◽  
ANTONELLA SCORDAMAGLIA ◽  
PAOLO FERRERO ◽  
MONICA SALVEGO ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
S Phase ◽  
Retinoblastoma Gene ◽  
Human Breast ◽  
Phase Index

Modulation of Bcl-2 and Ki-67 expression in oestrogen receptor-positive human breast cancer by tamoxifen

1994 ◽  
Vol 30 (11) ◽  
pp. 1663-1669 ◽  
Author(s):  
S.R.D. Johnston ◽  
K.A. MacLennan ◽  
N.P.M. Sacks ◽  
J. Salter ◽  
I.E. Smith ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Oestrogen Receptor ◽  
Human Breast ◽  
Ki 67
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