Adipose‐derived stem cells‐conditioned medium improved osteogenic differentiation of induced pluripotent stem cells when grown on polycaprolactone nanofibers

2018 ◽  
Vol 234 (7) ◽  
pp. 10315-10323 ◽  
Author(s):  
Fatemeh Soleimanifar ◽  
Fatemeh Sadat Hosseini ◽  
Hadi Atabati ◽  
Asma Behdari ◽  
Ladan Kabiri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Conditioned Medium ◽  
Pluripotent Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Induced Pluripotent

Effects of induced pluripotent stem cells-derived conditioned medium on the proliferation and anti-apoptosis of human adipose-derived stem cells

2016 ◽  
Vol 413 (1-2) ◽  
pp. 69-85 ◽  
Author(s):  
Rui-Ling Lian ◽  
Xiao-Ling Guo ◽  
Jian-Su Chen ◽  
Yong-Long Guo ◽  
Jia-Fu Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Conditioned Medium ◽  
Pluripotent Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Induced Pluripotent

scholarly journals Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Shih-Hsuan Mao ◽  
Chih-Hao Chen ◽  
Chien-Tzung Chen
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Nude Mice ◽  
Pluripotent Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Germ Layers ◽  
Cell Source ◽  
Induced Pluripotent

Abstract Background Bone regeneration is a crucial and challenging issue in clinical practice. Bone tissue engineering (BTE) with an optimal cell source may provide an ideal strategy for the reconstruction of bone defects. This study examined whether induced pluripotent stem cells (iPSCs) derived from adipose-derived stem cells (ASCs) could act as an osteogenic substitute and whether these ASC-iPSCs yield more new bone formation than ASCs in hydrogel scaffolds. Methods ASC-iPSCs were reprogrammed from ASCs through a retroviral system. ASCs were harvested and isolated from adipose tissue of humans. An aliquot of cell suspension (1 × 106 cells/mL) was seeded directly onto the nHAP-gelatin cryogel scaffolds. Nude mice back implantation of cell-seeded scaffolds was designed for in vivo comparison of osteogenic potentials between ASCs and ASC-iPSCs. Samples were harvested 4 and 8 weeks after implantation for further analysis based on histology and RT-PCR. Results ASC-iPSCs were successfully obtained from human adipose-derived stem cells. PCR results also showed that specific genes of iPSCs with the ability to cause the differentiation of cells into the three germ layers were expressed. In our in vivo experiments, iPSCs were subcutaneously injected into nude mice to induce teratoma formation. The morphology of the three germ layers was confirmed by histological staining. ASC is an essential cell source for BTE with benefits of high volume and less-invasive acquisition. With additional transforming Yamanaka factors, ASC-iPSCs showed higher osteogenic differentiation and elevated expression of collagen type I (Col I), osteocalcin (OCN), alkaline phosphate (ALP), and runt-related transcription factor 2 (RunX-2). Conclusions This report suggests that ASC-iPSCs could be a superior cell source in BTE with better osteogenic differentiation efficacy for future clinical applications.

Promoting osteogenic differentiation of human‐induced pluripotent stem cells by releasing Wnt/β‐catenin signaling activator from the nanofibers

2018 ◽  
Vol 120 (4) ◽  
pp. 6339-6346 ◽  
Author(s):  
Fatemeh Sadat Hosseini ◽  
Fatemeh Soleimanifar ◽  
Arash Khojasteh ◽  
Abdolreza Ardeshirylajimi
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

Improved osteogenic differentiation of human induced pluripotent stem cells cultured on polyvinylidene fluoride/collagen/platelet‐rich plasma composite nanofibers

2019 ◽  
Vol 235 (2) ◽  
pp. 1155-1164 ◽  
Author(s):  
Mohammad Foad Abazari ◽  
Fatemeh Soleimanifar ◽  
Mojdeh Amini Faskhodi ◽  
Reyhaneh Nassiri Mansour ◽  
Javad Amini Mahabadi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Polyvinylidene Fluoride ◽  
Composite Nanofibers ◽  
Platelet Rich Plasma ◽  
Induced Pluripotent ◽  
Cells Cultured

Melanocyte Differentiation From Induced Pluripotent Stem Cells Derived From Human Adipose-Derived Stem Cells

2019 ◽  
Vol 82 ◽  
pp. S119-S125
Author(s):  
Wen-Shyan Huang ◽  
Lin-Gwei Wei ◽  
Jhen-Kai Li ◽  
Keng-Yen Fu ◽  
Tai-Chun Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Induced Pluripotent

Generation of Induced Pluripotent Stem Cells from Human Adipose-Derived Stem Cells Without c-MYC

2010 ◽  
Vol 16 (7) ◽  
pp. 2197-2206 ◽  
Author(s):  
Tetsuhiro Aoki ◽  
Hiroe Ohnishi ◽  
Yasuaki Oda ◽  
Mika Tadokoro ◽  
Mari Sasao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Induced Pluripotent

scholarly journals Dysregulation of Mitochondrial Functions and Osteogenic Differentiation in Cisd2-Deficient Murine Induced Pluripotent Stem Cells

2015 ◽  
Vol 24 (21) ◽  
pp. 2561-2576 ◽  
Author(s):  
Ping-Hsing Tsai ◽  
Yueh Chien ◽  
Jen-Hua Chuang ◽  
Shih-Jie Chou ◽  
Chian-Hsu Chien ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Mitochondrial Functions ◽  
Induced Pluripotent

Osteogenic Differentiation of Induced Pluripotent Stem Cells on Electrospun Nanofibers: A Review of Literature

2020 ◽  
Vol 25 ◽  
pp. 101561
Author(s):  
Javad Hashemi ◽  
Ghasem Barati ◽  
Seyed Ehsan Enderami ◽  
Mohammadreza Safdari
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Electrospun Nanofibers ◽  
Review Of Literature ◽  
Induced Pluripotent

scholarly journals Mineralized gelatin methacrylate-based matrices induce osteogenic differentiation of human induced pluripotent stem cells

2014 ◽  
Vol 10 (12) ◽  
pp. 4961-4970 ◽  
Author(s):  
Heemin Kang ◽  
Yu-Ru V. Shih ◽  
Yongsung Hwang ◽  
Cai Wen ◽  
Vikram Rao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

Incorporated‐bFGF polycaprolactone/polyvinylidene fluoride nanocomposite scaffold promotes human induced pluripotent stem cells osteogenic differentiation

2019 ◽  
Vol 120 (10) ◽  
pp. 16750-16759 ◽  
Author(s):  
Mohammad Foad Abazari ◽  
Fatemeh Soleimanifar ◽  
Seyed Ehsan Enderami ◽  
Mahsa Nematzadeh ◽  
Navid Nasiri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Polyvinylidene Fluoride ◽  
Induced Pluripotent ◽  
Nanocomposite Scaffold
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