Micrococcal Nuclease stimulates Staphylococcus aureus Biofilm Formation in a Murine Implant Infection Model

Advancements in contemporary medicine have led to an increasing life expectancy which has broadened the application of biomaterial implants. As each implant procedure has an innate risk of infection, the number of biomaterial-associated infections keeps rising. Staphylococcus aureus causes 34% of such infections and is known as a potent biofilm producer. By secreting micrococcal nuclease S. aureus is able to escape neutrophil extracellular traps by cleaving their DNA-backbone. Also, micrococcal nuclease potentially limits biofilm growth and adhesion by cleaving extracellular DNA, an important constituent of biofilms. This study aimed to evaluate the impact of micrococcal nuclease on infection persistence and biofilm formation in a murine biomaterial-associated infection-model with polyvinylidene-fluoride mesh implants inoculated with bioluminescent S. aureus or its isogenic micrococcal nuclease deficient mutant. Supported by results based on in-vivo bioluminescence imaging, ex-vivo colony forming unit counts, and histological analysis it was found that production of micrococcal nuclease enables S. aureus bacteria to evade the immune response around an implant resulting in a persistent infection. As a novel finding, histological analysis provided clear indications that the production of micrococcal nuclease stimulates S. aureus to form biofilms, the presence of which extended neutrophil extracellular trap formation up to 13 days after mesh implantation. Since micrococcal nuclease production appeared vital for the persistence of S. aureus biomaterial-associated infection, targeting its production could be a novel strategy in preventing biomaterial-associated infection.

Enhanced Electrochemical Properties of Na0.67MnO2 Cathode for Na-Ion Batteries Prepared with Novel Tetrabutylammonium Alginate Binder

Both the binder and solid–electrolyte interface play an important role in improving the cycling stability of electrodes for Na-ion batteries. In this study, a novel tetrabutylammonium (TBA) alginate binder is used to prepare a Na0.67MnO2 electrode for sodium-ion batteries with improved electrochemical performance. The ageing of the electrodes is characterized. TBA alginate-based electrodes are compared to polyvinylidene fluoride- (PVDF) and Na alginate-based electrodes and show favorable electrochemical performance, with gravimetric capacity values of up to 164 mAh/g, which is 6% higher than measured for the electrode prepared with PVDF binder. TBA alginate-based electrodes also display good rate capability and improved cyclability. The solid–electrolyte interface of TBA alginate-based electrodes is similar to that of PVDF-based electrodes. As the only salt of alginic acid soluble in non-aqueous solvents, TBA alginate emerges as a good alternative to PVDF binder in battery applications where the water-based processing of electrode slurries is not feasible, such as the demonstrated case with Na0.67MnO2.

Development of Flexible Biceps Tremors Sensing Chip of PVDF Fibers with Nano-Silver Particles by Near-Field Electrospinning

Polyvinylidene fluoride (PVDF) and AgNO3/PVDF composite piezoelectric fibers were prepared using near-field electrospinning technology. The prepared fibers are attached to the electrode sheet and encapsulated with polydimethylsiloxane to create an energy acquisition device and further fabricated into a dynamic sensing element. The addition of AgNO3 significantly increased the conductivity of the solution from 40.33 μS/cm to 883.59 μS/cm, which in turn made the fiber drawing condition smoother with the increase of high voltage electric field and reduced the fiber wire diameter size from 0.37 μm to 0.23 μm. The tapping test shows that the voltage signal can reach ~0.9 V at a frequency of 7 Hz, and the energy conversion efficiency is twice that of the PVDF output voltage. The addition of AgNO3 effectively enhances the molecular bonding ability, which effectively increases the piezoelectric constants of PVDF piezoelectric fibers. When the human body is exercised for a long period of time and the body is overloaded, the biceps muscle is found to produce 8 to 16 tremors/second through five arm flexion movements. The voltage output of the flexible dynamic soft sensor is between 0.7–0.9 V and shows an orderly alternating current waveform of voltage signals. The sensor can be used to detect muscle tremors after high-intensity training and to obtain advance information about changes in the symptoms of fasciculation, allowing for more accurate diagnosis and treatment.

Tribological Properties of Carbon Nanotube and Carbon Nanofiber Blended Polyvinylidene Fluoride Sheets Laminated on Steel Substrates

Nanostructured carbon dispersed polymer nanocomposites are promising materials for tribological applications. Carbon nanofiber (CNF) and carbon nanotube (CNT) dispersed polyvinylidene fluoride (PVDF) nanocomposite was prepared by chemical synthesis route. Morphology and microstructure of well-dispersed CNF and CNT in PVDF were specified by scanning electron microscope and X-ray diffraction, respectively. Moreover, chemical and functional characteristics were examined by Raman spectroscopy and FTIR investigation. The friction coefficient of PVDF nanocomposite laminated on steel substrate decreased with an increase in the dispersed quantity of CNF and CNT. The friction coefficient of PVDF is approximately 0.27; however, the addition of carbon nanomaterial in PVDF will further decrease the friction coefficient between 0.24 and 0.17. This value was significantly less in CNT dispersed PVDF nanocomposite. This could be explained by easy shearing and rolling action contact interfaces.