Phaseolus vulgaris leucoagglutinin (PHA-L): A neuroanatomical tracer for electron microscopic analysis of synaptic circuitry in the cat's dorsal lateral geniculate nucleus

1990 ◽  
Vol 15 (4) ◽  
pp. 352-368 ◽  
Author(s):  
Josephine B. Cucchiaro ◽  
Daniel J. Uhlrich
Keyword(s):  
Phaseolus Vulgaris ◽  
Lateral Geniculate Nucleus ◽  
Microscopic Analysis ◽  
Electron Microscopic Analysis ◽  
Dorsal Lateral Geniculate Nucleus ◽  
Electron Microscopic ◽  
Lateral Geniculate ◽  
Dorsal Lateral Geniculate ◽  
Phaseolus Vulgaris Leucoagglutinin ◽  
Synaptic Circuitry

Quantitative immunogold evidence for enrichment of glutamate but not aspartate in synaptic terminals of retino-geniculate, geniculo-cortical, and cortico-geniculate axons in the cat

1994 ◽  
Vol 11 (4) ◽  
pp. 675-681 ◽  
Author(s):  
Vicente M. Montero
Keyword(s):  
Lateral Geniculate Nucleus ◽  
Glial Cells ◽  
Dorsal Lateral Geniculate Nucleus ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Selective Enrichment ◽  
Perigeniculate Nucleus ◽  
Synaptic Terminals ◽  
Lateral Geniculate ◽  
Dorsal Lateral Geniculate

AbstractA postembedding immunogold procedure was used on thin sections of the dorsal lateral geniculate nucleus (LGN) and perigeniculate nucleus (PGN) of the cat to estimate qualitatively and quantitatively, at the electron-microscopic (EM) level, the intensity of glutamate or aspartate immunoreactivities on identifiable synaptic terminals and other profiles of the neuropil. On sections incubated with a glutamate antibody, terminals of retinal and cortical axons in the LGN, and of collaterals of geniculo-cortical axons in the PGN, contain significantly higher density of immunogold particles than GABAergic terminals, glial cells, dendrites, and cytoplasm of geniculate cells. By contrast, in sections incubated with an aspartate antibody, terminals of retino-geniculate, cortico-geniculate, and geniculo-cortical axons did not show a selective enrichment of immunoreactivity, but instead the density of immunogold particles was generally low in the different profiles of the neuropil, with the exception of nucleoli. These results suggest that glutamate, but not aspartate, is a neurotransmitter candidate in the retino-geniculo-cortical pathways.

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