Adenoviral vector platform for transduction of constitutive and regulated tricistronic or triple-transcript transgene expression in mammalian cells and microtissues

2006 ◽  
Vol 8 (10) ◽  
pp. 1208-1222 ◽  
Author(s):  
Valeria Gonzalez-Nicolini ◽  
Carlota Diaz Sanchez-Bustamante ◽  
Shizuka Hartenbach ◽  
Martin Fussenegger
Keyword(s):  
Mammalian Cells ◽  
Transgene Expression ◽  
Adenoviral Vector ◽  
Expression In Mammalian Cells

scholarly journals Adeno-associated viral vectors engineered for macrolide-adjustable transgene expression In mammalian cells and mice

2007 ◽  
Vol 7 (1) ◽  
pp. 75 ◽  
Author(s):  
David A Fluri ◽  
Marie Baba ◽  
Martin Fussenegger
Keyword(s):  
Mammalian Cells ◽  
Transgene Expression ◽  
Viral Vectors ◽  
Expression In Mammalian Cells

scholarly journals The food additive vanillic acid controls transgene expression in mammalian cells and mice

2011 ◽  
Vol 40 (5) ◽  
pp. e37-e37 ◽  
Author(s):  
Marc Gitzinger ◽  
Christian Kemmer ◽  
David A. Fluri ◽  
Marie Daoud El-Baba ◽  
Wilfried Weber ◽  
...  
Keyword(s):  
Vanillic Acid ◽  
Mammalian Cells ◽  
Transgene Expression ◽  
Food Additive ◽  
Expression In Mammalian Cells

scholarly journals Versatile multi-transgene expression using improved BAC TG-EMBED toolkit, novel BAC episomes, and BAC-MAGIC

2019 ◽  
Author(s):  
Binhui Zhao ◽  
Pankaj Chaturvedi ◽  
David L. Zimmerman ◽  
Andrew S. Belmont
Keyword(s):  
Mammalian Cells ◽  
Transgene Expression ◽  
Large Fraction ◽  
Fine Tuning ◽  
Cmv Promoter ◽  
Chromosome Position ◽  
High Level ◽  
Genomic Regions ◽  
Expression In Mammalian Cells

ABSTRACTAchieving reproducible, stable, and high-level transgene expression in mammalian cells remains problematic. Previously, we attained copy-number-dependent, chromosome-position-independent expression of reporter minigenes by embedding them within a BAC containing the mouseMsh3-Dhfrlocus (DHFR BAC). Here we extend this “BAC TG-EMBED” approach. First, we report a toolkit of endogenous promoters capable of driving transgene expression over a 0.01-5 fold expression range relative to the CMV promoter, allowing fine-tuning of relative expression levels of multiple reporter genes expressed on a single BAC. Second, we show small variability in both the expression level and long-term expression stability of a reporter gene embedded in BACs containing either transcriptionally active or inactive genomic regions, making choice of BACs more flexible. Third, we describe an intriguing phenomenon in which BAC transgenes are maintained as episomes in a large fraction of stably selected clones. Finally, we demonstrate the utility of BAC TG-EMBED by simultaneously labeling three nuclear compartments in 94% of stable clones using a multi-reporter DHFR BAC, constructed with a combination of synthetic biology and BAC recombineering tools. Our extended BAC TG-EMBED method provides a versatile platform for achieving reproducible, stable simultaneous expression of multiple transgenes maintained either as episomes or stably integrated copies.

Adenovirus-Mediated Transduction of Auto- and Dual-Regulated Transgene Expression in Mammalian Cells

2008 ◽  
pp. 221-237
Author(s):  
Valeria Gonzalez-Nicolini ◽  
Martin Fussenegger
Keyword(s):  
Mammalian Cells ◽  
Transgene Expression ◽  
Expression In Mammalian Cells

A novel vector platform for vitamin H-inducible transgene expression in mammalian cells

2007 ◽  
Vol 131 (2) ◽  
pp. 150-158 ◽  
Author(s):  
Wilfried Weber ◽  
William Bacchus ◽  
Franziska Gruber ◽  
Mattia Hamberger ◽  
Martin Fussenegger
Keyword(s):  
Mammalian Cells ◽  
Transgene Expression ◽  
Expression In Mammalian Cells

Magnetically triggered transgene expression in mammalian cells by localized cellular heating of magnetic nanoparticles

2019 ◽  
Vol 128 (3) ◽  
pp. 355-364 ◽  
Author(s):  
Akira Ito ◽  
Ryoji Teranishi ◽  
Kazuki Kamei ◽  
Masaki Yamaguchi ◽  
Akihiko Ono ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Mammalian Cells ◽  
Transgene Expression ◽  
Expression In Mammalian Cells

Gas-inducible transgene expression in mammalian cells and mice

2004 ◽  
Vol 22 (11) ◽  
pp. 1440-1444 ◽  
Author(s):  
Wilfried Weber ◽  
Markus Rimann ◽  
Manuela Spielmann ◽  
Bettina Keller ◽  
Marie Daoud-El Baba ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Transgene Expression ◽  
Expression In Mammalian Cells
Sign in / Sign up

Export Citation Format

Share Document