Quantification of human herpesvirus 8 by real-time PCR in blood fractions of AIDS patients with Kaposi's sarcoma and multicentric Castleman's disease

2002 ◽  
Vol 68 (3) ◽  
pp. 399-403 ◽  
Author(s):  
Guy Boivin ◽  
Stéphanie Côté ◽  
Nathalie Cloutier ◽  
Yacine Abed ◽  
Michelle Maguigad ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Kaposi's Sarcoma ◽  
Castleman’S Disease ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Aids Patients ◽  
Herpesvirus 8 ◽  
Multicentric Castleman's Disease

scholarly journals Simultaneous quantification of human herpesvirus 8 DNA by real time PCR in different tissues of HIV infected cuban patients with Kaposi's sarcoma

Herpesviridae ◽  
2010 ◽  
Vol 1 (1) ◽  
pp. 3 ◽  
Author(s):  
Vivian Kourí ◽  
Pedro A Martínez ◽  
Orestes Blanco ◽  
Virginia Capó ◽  
María E Rodríguez ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Simultaneous Quantification ◽  
Different Tissues

Detection of human herpesvirus 8 DNA and antibodies to latent nuclear and lytic-phase antigens in serial samples from aids patients with Kaposi’s sarcoma

2000 ◽  
Vol 16 (3) ◽  
pp. 247-251 ◽  
Author(s):  
Lı́gia Camera Pierrotti ◽  
Laura Masami Sumita ◽  
Wilton Santos Freire ◽  
Hélio Hehl Caiaffa Filho ◽  
Vanda Akico Ueda Fick de Souza
Keyword(s):  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Aids Patients ◽  
Herpesvirus 8 ◽  
Serial Samples

Cytomegalovirus and human herpesvirus 8 DNA detection in peripheral blood monocytic cells of AIDS patients: Correlations with the presence of Kaposi's sarcoma and CMV disease

2005 ◽  
Vol 76 (4) ◽  
pp. 541-546 ◽  
Author(s):  
Antoinette C. van der Kuyl ◽  
Abeltje M. Polstra ◽  
Remco van den Burg ◽  
Gerrit Jan Weverling ◽  
Jaap Goudsmit ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Kaposi's Sarcoma ◽  
Dna Detection ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Aids Patients ◽  
Monocytic Cells ◽  
Herpesvirus 8 ◽  
Cmv Disease

scholarly journals Quantitation of Cell-Free and Cell-Associated Kaposi's Sarcoma-Associated Herpesvirus DNA by Real-Time PCR

2000 ◽  
Vol 38 (5) ◽  
pp. 1992-1995 ◽  
Author(s):  
Irene E. White ◽  
Thomas B. Campbell
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Kaposi's Sarcoma ◽  
Dynamic Range ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Linear Dynamic Range ◽  
Pcr Assay ◽  
Herpesvirus 8 ◽  
Linear Dynamic

A real-time PCR assay for quantitation of Kaposi's sarcoma-associated herpes virus (KSHV or human herpesvirus 8) DNA was evaluated. The linear dynamic range was 10 to 105 copies of KSHV DNA (r 2 > 0.99). The accuracy of DNA purification and quantitation was less than ±0.4 log10copies for samples that contained from 10 to 105 copies of KSHV DNA. Cell-associated KSHV DNA was quantitated over a range of infected cell frequencies from 0.1 to 10−5, and cell-free KSHV DNA in plasma was quantitated over a range of 100 to 106 copies/ml. Real-time PCR provides a convenient method for quantitation of cell-free and cell-associated KSHV DNA in laboratory and clinical specimens.

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