Molecular characterization of VP6-encoding gene of group A human rotavirus samples from central west region of Brazil

ABSTRACT A reverse transcription-PCR (RT-PCR) was established to amplify a 379-bp cDNA fragment (nucleotides 747 to 1126, coding for amino acids 241 to 367) of the VP6 gene of group A rotaviruses associated with subgroup (SG) specificity. Thirty-eight human rotavirus strains characterized with SG-specific monoclonal antibodies were subjected to VP6-specific RT-PCR, and PCR amplicons were used for sequencing. Nucleic acid sequencing and phylogenetic analysis of the VP6 amplicons revealed two clusters, or genogroups. Two genetic lineages were distinguished within genogroup I, consisting of strains serologically characterized as SG I, and three genetic lineages were distinguished within genogroup II, composed of strains serologically characterized as SG II, SG I + II, and SG non-I, non-II. Subgrouping of rotaviruses by means of serological methods may result in strains not being assigned the correct SG or in a failure of strains to subgroup. Molecular characterization of the SG-defining region of VP6 provided evidence for independent segregation of the rotavirus genes encoding VP4, VP6, and VP7.

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Molecular characterization of hasB from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDP-glucose dehydrogenase activity.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)53153-7 ◽

1993 ◽

Vol 268 (10) ◽

pp. 7118-7124

Author(s):

B.A. Dougherty ◽

I. van de Rijn

Keyword(s):

Hyaluronic Acid ◽

Dehydrogenase Activity ◽

Molecular Characterization ◽

Glucose Dehydrogenase ◽

Acid Synthesis ◽

Group A Streptococci ◽

Group A

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Molecular characterization of the first human G15 rotavirus strain of zoonotic origin from the bovine species

Journal of General Virology ◽

10.1099/jgv.0.001581 ◽

2021 ◽

Vol 102 (4) ◽

Author(s):

Takeshi Tsugawa ◽

Yoshiki Fujii ◽

Yusuke Akane ◽

Saho Honjo ◽

Kenji Kondo ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Molecular Characterization ◽

Human Infection ◽

Potential Reservoir ◽

Group A Rotaviruses ◽

Paediatric Outpatient ◽

Group A ◽

Human Infections ◽

Bovine Species

Group A rotaviruses (RVAs) infect a wide variety of mammalian and avian species. Animals act as a potential reservoir to RVA human infections by direct virion transmission or by contributing genes to reassortants. Here, we report the molecular characterization of a rare human RVA strain Ni17-46 with a genotype G15P[14], isolated in Japan in 2017 during rotavirus surveillance in a paediatric outpatient clinic. The genome constellation of this strain was G15-P[14]-I2-R2-C2-M2-A13-N2-T9-E2-H3. This is the first report of an RVA with G15 genotype in humans, and sequencing and phylogenetic analysis results suggest that human infection with this strain has zoonotic origin from the bovine species. Given the fact that this strain was isolated from a patient with gastroenteritis and dehydration symptoms, we must take into account the virulence of this strain in humans.

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Characterization of the NSP4 gene of group A human rotavirus G1P[8] strains circulating in Sapporo, Japan from 1987 to 2000

Journal of Medical Virology ◽

10.1002/jmv.23723 ◽

2013 ◽

Vol 86 (2) ◽

pp. 354-359 ◽

Cited By ~ 5

Author(s):

Masatoshi Tatsumi ◽

Yoshinobu Nagaoka ◽

Takeshi Tsugawa ◽

Yuko Yoto ◽

Tsukasa Hori ◽

...

Keyword(s):

Nsp4 Gene ◽

Human Rotavirus ◽

Group A

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Molecular characterization of rotavirus strains detected during a clinical trial of a human rotavirus vaccine in Blantyre, Malawi

Vaccine ◽

10.1016/j.vaccine.2011.09.119 ◽

2012 ◽

Vol 30 ◽

pp. A140-A151 ◽

Cited By ~ 12

Author(s):

Toyoko Nakagomi ◽

Osamu Nakagomi ◽

Winifred Dove ◽

Yen Hai Doan ◽

Desiree Witte ◽

...

Keyword(s):

Clinical Trial ◽

Molecular Characterization ◽

Rotavirus Vaccine ◽

Human Rotavirus

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Molecular characterization of group A rotaviruses detected in children with gastroenteritis in Ireland in 2006–2009

Epidemiology and Infection ◽

10.1017/s0950268811000306 ◽

2011 ◽

Vol 140 (2) ◽

pp. 247-259 ◽

Cited By ~ 18

Author(s):

O. CASHMAN ◽

P. J. COLLINS ◽

G. LENNON ◽

B. CRYAN ◽

V. MARTELLA ◽

...

Keyword(s):

Sequence Analysis ◽

Irish Population ◽

Surveillance Activity ◽

Human Rotavirus ◽

Group A Rotaviruses ◽

Group A ◽

Hospital Acquired ◽

Rotavirus Infections ◽

Pcr Genotyping

SUMMARYCommunity and hospital-acquired cases of human rotavirus are responsible for millions of gastroenteritis cases in children worldwide, chiefly in developing countries, and vaccines are now available. During surveillance activity for human rotavirus infections in Ireland, between 2006 and 2009, a total of 420 rotavirus strains were collected and analysed. Upon either PCR genotyping and sequence analysis, a variety of VP7 (G1–G4 and G9) and VP4 (P[4], P[6], P[8] and P[9]) genotypes were detected. Strains G1P[8] were found to be predominant throughout the period 2006–2008, with slight fluctuations seen in the very limited samples available in 2008–2009. Upon either PCR genotyping and sequence analysis of selected strains, the G1, G3 and G9 viruses were found to contain E1 (Wa-like) NSP4 and I1 VP6 genotypes, while the analysed G2 strains possessed E2 NSP4 and I2 VP6 genotypes, a genetic make-up which is highly conserved in the major human rotavirus genogroups Wa- and Kun-like, respectively. Upon sequence analysis of the most common VP4 genotype, P[8], at least two distinct lineages were identified, both unrelated to P[8] Irish rotaviruses circulating in previous years, and more closely related to recent European humans rotaviruses. Moreover, sequence analysis of the VP7 of G1 rotaviruses revealed the onset of a G1 variant, previously unseen in the Irish population.

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