Bacterial Colonization in Computer Keyboards Posses Health Hazard

Computer keyboards of a teaching laboratory were examined and bacteria were isolated from computer keyboards. The subsequent tests were done for the bacterial isolates: methyl red, vogus proskaur, citrate utilization, urease and TSI. This study paves the way to look at an inanimate object like computer keyboard as potential reservoir of bacteria.

Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients

Glioblastoma (GB) is an aggressive type of tumour for which therapeutic options and biomarkers are limited. GB diagnosis mostly relies on symptomatic presentation of the tumour and, in turn, brain imaging and invasive biopsy that can delay its diagnosis. Description of easily accessible and effective biomarkers present in biofluids would thus prove invaluable in GB diagnosis. Extracellular vesicles (EVs) derived from both GB and stromal cells are essential to intercellular crosstalk in the tumour bulk, and circulating EVs have been described as a potential reservoir of GB biomarkers. Therefore, EV-based liquid biopsies have been suggested as a promising tool for GB diagnosis and follow up. To identify GB specific proteins, sEVs were isolated from plasma samples of GB patients as well as healthy volunteers using differential ultracentrifugation, and their content was characterised through mass spectrometry. Our data indicate the presence of an inflammatory biomarker signature comprising members of the complement and regulators of inflammation and coagulation including VWF, FCGBP, C3, PROS1, and SERPINA1. Overall, this study is a step forward in the development of a non-invasive liquid biopsy approach for the identification of valuable biomarkers that could significantly improve GB diagnosis and, consequently, patients’ prognosis and quality of life.

Structural analysis and seismic stratigraphy for delineation of Neoproterozoic-Cambrian petroleum system in central and eastern part of Bikaner–Nagaur basin, India

Bikaner–Nagaur basin is located in the northwestern part of India and lies on the rising flank of Punjab platform of Middle Indus basin in Pakistan. Existence of Neoproterozoic-Cambrian petroleum system was confirmed by the exploration activities in the western periphery of the basin, whereas vast areas of central and eastern parts remain unexplored. Knowledge of petroleum system in this unexplored part of the basin is limited due to non-availability of data. Recently, 2525 line km of regional 2D seismic data acquired for the first time by Government of India under National Seismic Program (NSP) unlocks the opportunity for comprehensive understanding of subsurface geology in unexplored part of the basin. Present work aims to interpret recently acquired 2D seismic data and integrate with available surface (outcrop) data, gravity and well data (drilled in western part of basin) for unfolding the petroleum system elements, structural configurations and stratigraphic features in the hitherto central-eastern part of the basin. Two Neoproterozoic-Cambrian hydrocarbon plays: (1) Jodhpur and (2) overlying Bilara/Hanseran Evaporite Group (HEG) were envisaged. Both the plays depicted distinctive seismic characteristics, structural alignment and distribution of reservoir, source and seal. Fluvio-deltaic sandstone within Jodhpur group and shallow marine fractured dolomites within Bilara/HEG showed potential reservoir characteristics whereas organic rich laminated dolomites, stromatolites and argillaceous litho-units within Bilara/HEG group have been predicted as prospective source. The Halite layers within HEG group were considered as effective regional seals. Fault bounded anticlinal structures associated with Cambrian compression have been identified as the main entrapment for hydrocarbon accumulation. The basin witnessed long tectonostratigraphic history with two major compressional phases Structures formed by Cambrian compression are likely to be charged as the time of source maturity and peak expulsion was later, during early Mesozoic period. Overall, the study indicates new opportunities and potential accumulation of hydrocarbon in the unexplored part of the basin.

Asymptomatic Scalp Carriage among Household Contacts of Children Affected by Tinea Capitis: A Prospective Study in the Metropolitan Area of Brussels, Belgium

<b><i>Introduction:</i></b> Tinea capitis (TC) is a superficial fungal infection affecting the scalp. The existence of asymptomatic carriers (ACs) could represent a potential reservoir responsible of (re)contamination and failure of treatment. No prospective studies on ACs in household contacts of TC patients in Europe have been published to date. <b><i>Objectives:</i></b> The aim of this study was to assess the prevalence of ACs in a cohort of household contacts of children who were diagnosed with TC in the metropolitan area of Bruxelles, Belgium. <b><i>Methods:</i></b> This prospective observational study was conducted from October 2015 to April 2016 at the Dermatology Department of the University Hospitals Brugmann, Saint-Pierre, Queen Fabiola Children Hospital. <b><i>Results:</i></b> Ninety-nine cases of TC from 95 different family circles were included. The main infectious agent identified was <i>Microsporum audouinii</i> in 53 cases. The mean age of TC patients was 5.8 years. Male/female ratio was 2.8. Eighty-one household contacts of TC patients were enrolled in the study. Two cases of ACs (5%) were identified. <b><i>Conclusions:</i></b> <i>M. audouinii</i> was the most common pathogen identified. The prevalence of ACs we report is on average higher compared to other European large cities. Larger prospective studies including all close contacts of affected patients are required in order to establish guidelines regarding identification and management of ACs.

Prevalence and antibiotic resistance of Salmonella isolated from poultry and its environment in the Mekong Delta, Vietnam

Background and Aim: Salmonella is one of the leading causes of zoonotic and foodborne infectious outbreaks in humans and poultry and its associated environment is a potential reservoir of Salmonella. In recent years, the antibiotic resistance of bacteria, including Salmonella, has been increasing. This study aimed to investigate the prevalence and antibiotic resistance of Salmonella isolated from poultry, its environment, and the pest animals found at poultry farms and households of the Mekong Delta, Vietnam. Materials and Methods: A total of 3,055 samples were collected from the broiler farms and households of the Mekong Delta from 2017 to 2020. Salmonella was isolated using conventional methods (culturing on selective agar – BPLS and biochemical test) and the isolates were examined for antibiotic resistance against 14 antibiotics using the disk diffusion method. Results: Salmonella was isolated from 181 samples (5.92%), which included chicken feces (7.67%), pest animals (5.98%), and environmental samples (4.33%). The environmental samples comprised bedding (5.88%), feed (5.48%), and drinking water (0.70%). The prevalence of Salmonella was the highest in rats (15.63%) and geckos (12.25%) followed by ants (2.83%) and cockroaches (2.44%); however, Salmonella was not isolated from any fly species. Most of the isolates exhibited resistance to 1-9 antibiotics. The isolates were relatively resistant to chloramphenicol (62.98%), tetracycline (55.80%), ampicillin (54.14%), and sulfamethoxazole/trimethoprim (53.04%). Sixty-two multiple resistance patterns were found in the isolates, with ampicillin-cefuroxime-chloramphenicol-tetracycline- sulfamethoxazole/trimethoprim being the most frequent (7.18%). Conclusion: The chickens, husbandry environment, and pest animals at poultry farms and households were found to be important Salmonella sources in the Mekong Delta. Salmonella isolates from these sources also exhibited a wide-ranging resistance to antibiotics as well as several resistance patterns. Hence, biosecurity should be addressed in poultry farms and households to prevent cross-contamination and reduce the spread of Salmonella infections.

Species identification and phylogenetic analysis of Leishmania isolated from patients, vectors and hares in the Xinjiang Autonomous Region, The People’s Republic of China

Background Visceral leishmaniasis (VL) has been declared as one of the six major tropical diseases by the World Health Organization. This disease has been successfully controlled in China, except for some areas in the western region, such as the Xinjiang Autonomous Region, where both anthroponotic VL (AVL) and desert type zoonotic VL (DT-ZVL) remain endemic with sporadic epidemics. Methodology/Principal findings Here, an eleven-year survey (2004–2014) of Leishmania species, encompassing both VL types isolated from patients, sand-fly vectors and Tarim hares (Lepus yarkandensis) from the Xinjiang Autonomous Region was conducted, with a special emphasis on the hares as a potential reservoir animal for DT-ZVL. Key diagnostic genes, ITS1, hsp70 and nagt (encoding N-acetylglucosamine-1-phosphate transferase) were used for phylogenetic analyses, placing all Xinjiang isolates into one clade of the L. donovani complex. Unexpectedly, AVL isolates were found to be closely related to L. infantum, while DT-ZVL isolates were closer to L. donovani. Unrooted parsimony networks of haplotypes for these isolates also revealed their relationship. Conclusions/Significance The above analyses of the DT-ZVL isolates suggested their geographic isolation and independent evolution. The sequence identity of isolates from patients, vectors and the Tarim hares in a single DT-ZVL site provides strong evidence in support of this species as an animal reservoir.

Genome Size, Cytotype Diversity and Reproductive Mode Variation of Cotoneaster integerrimus (Rosaceae) from the Balkans

Cotoneaster integerrimus represents a multiploid and facultative apomictic system of widely distributed mountain populations. We used flow cytometry to determine genome size, ploidy level, and reproduction mode variation of the Balkan populations, supplemented by analysis of nuclear microsatellites in order to address: (i) geographic distribution and variation of cytotypes among the populations; (ii) variation of reproduction mode and the frequency of sexuality; (iii) pathways of endosperm formation among the sampled polyploids and their endosperm balance requirements; (iv) genotypic diversity and geographic distribution of clonal lineages of polyploids. The prevalence of apomictic tetraploid cytotype followed by sexual diploids and extremely rare triploids was demonstrated. This prevalence of tetraploids affected the populations’ structure composed from clonal genotypes with varying proportions. The co-occurrence of diploids and tetraploids generated higher cytotype, reproductive mode, and genotypic diversity, but mixed-ploidy sites were extremely rare. The endosperm imbalance facilitates the development and the occurrence of intermediate triploids in mixed-ploidy populations, but also different tetraploid lineages elsewhere with unbalanced endosperm. All these results showed that the South European populations of C. integerrimus have higher levels of cytotype and reproductive diversity compared to the Central European ones. Therefore, the South European populations can be considered as a potential reservoir of regional and global diversity for this species.

De-Risking Fluid Compartmentalization of the Barik Reservoir in the Khazzan Field, Oman - An Integrated Approach

The degree of fluid compartmentalization has direct implications on the development costs of oil and gas reservoirs, since it may negatively impact gas water contacts (GWC) and fluid condensate gas ratios (CGR). In this case study on the Barik Formation in the giant Khazzan gas field in Block 61 in Oman we demonstrate how integrating independent approaches for assessing potential reservoir compartmentalization can be used to assess compartmentalization risk. The three disciplines that were integrated are structural geology (fault seal analysis, movement and stress stages of faults and fractures, traps geometry over geological time), petroleum systems (fluid chemistry and pressure, charge history) and sedimentology-stratigraphy including diagenesis (sedimentological and diagenetic controls on vertical and lateral facies and reservoir quality variation). Dynamic data from production tests were also analyzed and integrated with the observations above. Based on this work, Combined Common Risk Segment (CCRS) maps with a most likely and alternative scenarios for reservoir compartmentalization were constructed. While pressure data carry significant uncertainty due to the tight nature of the deeply buried rocks, it is clear pressures in gas-bearing sections fall onto a single pressure gradient across Block 61, while water pressures indicate variable GWCs. Overall, the GWCs appear to shallow across the field towards the NW, while water pressure appears to increase in that direction. The "apparent" gas communication with separate aquifers is difficult to explain conventionally. A range of scenarios for fluid distribution and reservoir connectivity are discussed. Fault seal compartmentalization and different trap spill points were found to be the most likely mechanism explaining fluid distribution and likely reservoir compartmentalization. Perched water may be another factor explaining variable GWCs. Hydrodynamic tilting due to the flow of formation water was deemed an unlikely scenario, and the risk of reservoir compartmentalization due to sedimentological and diagenetic flow barriers was deemed to be low.

Lepra Bubalorum, a Potential Reservoir of Mycobacterium leprae

In 1926, a mycobacterial skin disease was observed in water buffaloes by researchers in Indonesia. The disease was designated as skin tuberculosis, though it was hypothesized that it might be a form of leprosy or a leprosy-like disease. In a follow-up study (Ph.D. thesis Lobel, 1934, Utrecht University, Netherlands) a similar nodular skin disease was described in Indonesian water buffaloes and named “lepra bubalorum” or “nodular leprosy.” Two decades later Kraneveld and Roza (1954) reported that, so far, the diagnosis lepra bubalorum had been made in 146 cases in Indonesia. After a final series of research reports by Indonesian veterinarians in 1961, no subsequent cases were published. Based on information from these reports, it can be concluded that, even though evidence of nerve involvement in buffaloes was not reported, similarities exist between lepra bubalorum and Hansen’s disease (leprosy), i.e., nodular skin lesions with a chronic course and microscopically granulomatous reactions with AFB in globi in vacuoles. This raises the question as to whether these historical cases might indeed have been caused by Mycobacterium leprae, Mycobacterium lepromatosis or another representative of the M. leprae complex. The future use of state-of-the-art molecular techniques may answer this question and may also help to answer the question whether water buffaloes should be considered as a potential natural reservoir of the causative pathogen of Hansen’s disease.

The Deep Water Field Alpha Plug and Abandonment Campaign – An Integrated Approach to Optimize Operations and Costs

After having been developed in the early 2000's and put on production since then, the deep water (700 M water depth) Field Alpha is now at the end of field life and in the plug and abandonment (P&A) process. Although this field-life phase does not make any money for an E&P operating company, it can be a liability and put the company's reputation at stake, if not done correctly. Therefore, like any other field-life phases, it requires a professional and multidisciplinary integrated approach to deliver it while reducing the company's exposure. P&A campaign involves many stake holders: the local authorities, its technical and operational representative / auditor, the operator's well engineering community, the subsurface team, e.g., petrophysics team, and the operator's management. Understanding all their expectations and KPIs is primordial to prepare and successfully deliver such operations. The P&A process relies on the placement of adequate "barriers" inside the well to guarantee blockade of any potential reservoir fluid communication either within separate reservoirs (to avoid any reservoir re-pressurization through cross flow) or with seabed / surface which could impact the environment. As part of the well barrier, annuli cement quality and efficiency must be checked by cement bond evaluation. The petrophysics team has the responsibility to both define, in collaboration with others team members, the cement quality criteria and then to evaluate the cement quality in timely and efficient manner. In this campaign, which is expected to run for more than a year, with several petrophysicists involved both in the preparation phase and in the operation phase, e.g., execution and results validation, consistency in the process is of utmost importance. This paper presents the workflow put in place by PETRONAS Carigali during the Field Alpha P&A campaign. It emphasizes on the petrophysicist role and responsibilities from the preparation phase, during the operations, and through the results validation. The learnings and experiences acquired during the Deep-Water Field Alpha P&A campaign are now going to be transpose to domestic and international assets by means of corporate guidelines and workflows.