Plasma membrane and vesicular glutamate transporter expression in chromaffin cells of bovine adrenal medulla

2010 ◽  
Vol 89 (1) ◽  
pp. 44-57 ◽  
Author(s):  
A.M. Oliván ◽  
R. Pérez-Rodríguez ◽  
C. Roncero ◽  
C. Arce ◽  
M.P. González ◽  
...  
1987 ◽  
Vol 243 (2) ◽  
pp. 541-547 ◽  
Author(s):  
E G Delicado ◽  
M T Miras Portugal

1. Isolated chromaffin cells from bovine adrenal medulla were used to study glucose transport in a homogeneous neural tissue. 2. The affinity of glucose transporters was 1.20 +/- 0.52 mM by the infinite-cis technique and 1.02 +/- 0.09 mM by the direct transport experiments. 3. The affinity for 2-deoxyglucose of these transporters was 2.3 mM. 4. The glucose transporters, quantified by [3H]cytochalasin B binding, were 419,532 +/- 120,740 receptors/cell, which corresponds to about 7.2 +/- 2 pmol/mg of protein, with KD = 0.1 microM. 5. High-affinity insulin receptors with KD = 3.95 nM were present at a density of 68,400 +/- 7500 per cell. 6. Insulin and secretagogues increased glucose transport, raising the transporter number at the plasma membrane without changes in the affinity.


Author(s):  
Fernando F. Vargas ◽  
Soledad Calvo ◽  
Raul Vinet ◽  
Eduardo Rojas

Biochimie ◽  
1994 ◽  
Vol 76 (5) ◽  
pp. 404-409 ◽  
Author(s):  
M.A. Günther Sillero ◽  
M. Del Valle ◽  
E. Zaera ◽  
P. Michelena ◽  
A.G. García ◽  
...  

2015 ◽  
Vol 590 ◽  
pp. 184-188 ◽  
Author(s):  
Joeri Van Liefferinge ◽  
Cathy J. Jensen ◽  
Giulia Albertini ◽  
Eduard Bentea ◽  
Thomas Demuyser ◽  
...  

2008 ◽  
Vol 284 (7) ◽  
pp. 4300-4307 ◽  
Author(s):  
Mahesh Darna ◽  
Isabelle Schmutz ◽  
Karin Richter ◽  
Sowmya V. Yelamanchili ◽  
Gurudutt Pendyala ◽  
...  

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