In vitro fertilization of hamster and human oocytes by microinjection of human sperm

1987 ◽  
Vol 16 (1) ◽  
pp. 69-78 ◽  
Author(s):  
B. Lassalle ◽  
A. M. Courtot ◽  
J. Testart
Keyword(s):  
In Vitro Fertilization ◽  
Human Sperm ◽  
Vitro Fertilization ◽  
Human Oocytes

scholarly journals Oolemma invagination of fresh and cryopreserved human oocytes during in vitro fertilization by ICSI

2018 ◽  
Vol 28 (3) ◽  
pp. 258-265 ◽  
Author(s):  
Maryna P. Petrushko ◽  
◽  
Taisiia O. Yurchuk ◽  
Natalia O. Buderatska ◽  
Volodymyr I. Piniaiev ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Human Oocytes

Quality control in an in-vitro fertilization laboratory: use of human sperm survival studies

1989 ◽  
Vol 4 (5) ◽  
pp. 545-549 ◽  
Author(s):  
J. Diane Critchlow ◽  
Phillip L. Matson ◽  
C. Newman Maureen ◽  
Gregory Horne ◽  
Stephen A. Troup ◽  
...  
Keyword(s):  
Quality Control ◽  
In Vitro Fertilization ◽  
Human Sperm ◽  
Vitro Fertilization ◽  
Sperm Survival ◽  
Survival Studies

Prematurely condensed human sperm chromosomes after in vitro fertilization (IVF)

1986 ◽  
Vol 74 (4) ◽  
pp. 441-443 ◽  
Author(s):  
H. Schmiady ◽  
K. Sperling ◽  
H. Kentenich ◽  
M. Stauber
Keyword(s):  
In Vitro Fertilization ◽  
Human Sperm ◽  
Vitro Fertilization ◽  
Sperm Chromosomes ◽  
Human Sperm Chromosomes

Development and implementation of intracytoplasmic sperm injection (ICSI)

1995 ◽  
Vol 7 (2) ◽  
pp. 211 ◽  
Author(s):  
GD Palermo ◽  
J Cohen ◽  
M Alikani ◽  
A Adler ◽  
Z Rosenwaks
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Human Sperm ◽  
Fertilization Rate ◽  
Semen Parameters ◽  
Ongoing Pregnancy Rate ◽  
Vitro Fertilization ◽  
Human Oocytes ◽  
Intracytoplasmic Injection ◽  
Previously Treated

The purpose of this paper is to elucidate the experimental steps that led to the development of intracytoplasmic sperm injection (ICSI) and its application in the human. ICSI has become the most successful micromanipulation procedure for treating male infertility. A total of 355 in vitro fertilization (IVF) cycles utilizing ICSI are described; 180 couples were previously treated in 509 IVF cycles but achieved no fertilization and 175 couples could not be treated by IVF because of extremely poor semen parameters. Of the 3063 metaphase II (M II) oocytes retrieved, 2970 were injected with a survival rate of 93.6%, yielding 1917 bipronuclear zygotes (64.5%). In 148 patients, a foetal heart was evidenced by ultrasound; 11 of these patients miscarried between 7 and 13 weeks of gestation. The ongoing pregnancy rate was 38.6% (137/355) per retrieval and 40.5% (137/338) per embryo replacement. At the time of writing, there were 22 deliveries and one therapeutic abortion for a trisomy 21 chromosomal abnormality. In addition, 66 singleton, 37 twin, 10 triplet and 1 quadruplet pregnancies were ongoing. The concentration of motile spermatozoa in the ejaculate only slightly influenced the fertilization rate (P < 0.001) and the pregnancy outcome (P < 0.01). A preliminary injection procedure utilizing intracytoplasmic injection of isolated sperm heads was performed in 35 M II human oocytes with resultant fertilization and cleavage rates of 74% and 73% respectively. Skills in ICSI were acquired by injecting hamster and unfertilized human oocytes with human sperm. ICSI can be used to successfully treat couples who have failed IVF or who have too few spermatozoa for conventional in vitro insemination.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Structural and morphologic differences in human oocytes after in vitro maturation compared with standard in vitro fertilization

2016 ◽  
Vol 106 (6) ◽  
pp. 1392-1398.e5 ◽  
Author(s):  
Melanie L. Walls ◽  
Roger Hart ◽  
Jeffrey A. Keelan ◽  
John P. Ryan
Keyword(s):  
In Vitro Fertilization ◽  
In Vitro Maturation ◽  
Vitro Fertilization ◽  
Human Oocytes

Timing of in-vitro fertilization of cumulus-free and cumulus-enclosed human oocytes

1986 ◽  
Vol 1 (4) ◽  
pp. 237-242 ◽  
Author(s):  
Michelle Plachot ◽  
Anne-Marie Junca ◽  
Jacqueline Mandelbaum ◽  
Jean Cohen ◽  
Jacques Salat-Baroux ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Human Oocytes

Cytogenetic and morphological observations of single pronucleated human oocytes after in-vitro fertilization

1993 ◽  
Vol 8 (2) ◽  
pp. 221-223 ◽  
Author(s):  
C. Staessen ◽  
C. Janssenswillen ◽  
P. Devroey ◽  
A.C.Van Steirteghem
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Human Oocytes
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