Toxicity Assessment of Energetic Materials by Using the Luminescent Bacteria Inhibition Test

The bioassay has been attracting attention as a method of toxicity assessments of micropollutants in the environment. In this study, we report the characteristics (selectivity and sensitivity) of the nitrifying bacteria biosensor for 255 kinds of chemicals as a model of chemical contaminant in the environment and the results of evaluation of mixed samples of several substances. In the nitrifying bacteria respiration inhibition test using the biosensor, 56 chemicals were detected. It was found that this biosensor is especially sensitive to seven chemicals that have a thiocarbonyl functional group (>C=S), such as a thioamide group of thiocarbamate group. These chemicals are considered to specifically inhibit AMO by chelation of copper. The samples consisted of a mixture of seven types of anilines that inhibit respiration in the bacteria, a mixture of five types of chlorophenols, and a mixture of eight types of substances that contain thiocarbonyl groups were examined. All of the mixed samples inhibited the respiration of the nitrifying bacteria more than 10% by the inhibition rate, and observed a synergistic effects of the substances in the samples.

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Acute toxicity assessment of indoor dust extracts by luminescent bacteria assays with Photobacterium Phosphoreum T3

Environmental Research ◽

10.1016/j.envres.2020.110447 ◽

2020 ◽

pp. 110447

Author(s):

Jiafan Li ◽

Tao Yuan ◽

Yuning Ma ◽

Zhemin Shen ◽

Ying Tian ◽

...

Keyword(s):

Acute Toxicity ◽

Toxicity Assessment ◽

Photobacterium Phosphoreum ◽

Indoor Dust ◽

Luminescent Bacteria

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Acute toxicity assessment of explosive-contaminated soil extracting solution by luminescent bacteria assays

Environmental Science and Pollution Research ◽

10.1007/s11356-016-7492-5 ◽

2016 ◽

Vol 23 (22) ◽

pp. 22803-22809 ◽

Cited By ~ 8

Author(s):

Wenjie Xu ◽

Zhenming Jiang ◽

Quanlin Zhao ◽

Zhenzhong Zhang ◽

Hongping Su ◽

...

Keyword(s):

Acute Toxicity ◽

Contaminated Soil ◽

Toxicity Assessment ◽

Luminescent Bacteria

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EFFECTIVENESS OF GELATIN EXTRACTED FROM CHICKEN CLAWS AS A NATURAL PRESERVATIVES FOR BEEF AND FISH

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.v10i2.544 ◽

2016 ◽

Vol 10 (2) ◽

pp. 195

Author(s):

Ganjar Fadilah ◽

Pramudita Putri Kusuma ◽

Teguh Endah Saraswati

Keyword(s):

Inhibition Test ◽

Oxidation Number ◽

Hydrolysis Method ◽

Texture Parameters ◽

Inhibitory Region ◽

Bacteria Inhibition ◽

Gelatin Concentration ◽

Natural Preservatives ◽

And Control ◽

Growth Of Bacteria

<p>Gelatin has been successfully extracted from chicken clawsby acid hydrolysis method through four major stages of degreasing, demineralization, extraction and drying. The yield of extracted gelatin obtained from wet chicken claws was about 6.21 % (w/w). The effectiveness of gelatin as preservative applied in beef and fish was physically, chemically and microbiologically studied by organoleptic analysis, peroxide test, bacteria inhibition test and number calculation, respectively. Organoleptic analysis by hedonic test shows there were significant differences for color and texture parameters between gelatin coated samples and control samples at concentrations of 1:1 (w/w). Peroxide number analysis by iodometry indicates the use of gelatin in the preservation of meat and fish decreased the oxidation number. The result of inhibition test againts Staphylococcus aureus bacteria shows the gelatin concentration of 20 % provided greatest halo zone diameter (inhibitory region) as 19.44 ± 2.11 mm. The colony bacteria test shows the use of gelatin potentially inhibited the growth of bacteria up to 75 %. Overall, the results conclude that gelatin extracted from chicken claws has the potential activities as a natural preservative for beef and fish.</p>

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Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment

Ciencias Marinas ◽

10.7773/cm.v32i12.1031 ◽

2006 ◽

Vol 32 (1B) ◽

pp. 129-138 ◽

Cited By ~ 11

Author(s):

MC Casado-Martínez

Keyword(s):

Environmental Quality ◽

Sediment Toxicity ◽

Toxicity Assessment ◽

Inhibition Test ◽

Coastal Sediments ◽

Bioluminescence Inhibition ◽

Sediment Toxicity Assessment

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Bacterial Toxicity Testing: Modification and Evaluation of the Luminescent Bacteria Test and the Respiration Inhibition Test

Processes ◽

10.3390/pr8111349 ◽

2020 ◽

Vol 8 (11) ◽

pp. 1349

Author(s):

Uwe Strotmann ◽

Daniel Pastor Flores ◽

Odorico Konrad ◽

Cornelia Gendig

Keyword(s):

Vibrio Fischeri ◽

Toxicity Testing ◽

Test System ◽

Inhibition Test ◽

Chlorinated Phenols ◽

Bivalent Cation ◽

Prolonged Incubation ◽

Test Systems ◽

Luminescent Bacteria ◽

Luminescent Bacteria Test

The activated sludge respiration inhibition test and the luminescent bacteria test with Vibrio fischeri are important bacterial test systems for evaluation of the toxicity of chemical compounds. These test systems were further optimized to result in better handling, reliability and sensitivity. Concerning the Vibrio fischeri test, media components such as yeast extract and bivalent cation concentrations like Ca2+ and Mg2+ were optimized. The cultivation, storage conditions and reactivation process of the stored bacteria were also improved, which enabled simpler handling and led to good reproducibility. Additionally, the respiration inhibition test with a prolonged incubation time was further analyzed using different chlorinated phenols as reference compounds. It could be stated that a longer incubation period significantly improved the sensitivity of the test system.

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