Derivatization of phosphorylated peptides with S- and N-nucleophiles for enhanced ionization efficiency in matrix-assisted laser desorption/ionization mass spectrometry

2004 ◽  
Vol 18 (22) ◽  
pp. 2697-2705 ◽  
Author(s):  
Clementine Klemm ◽  
Stephan Schröder ◽  
Matthias Glückmann ◽  
Michael Beyermann ◽  
Eberhard Krause
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Efficiency ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Phosphorylated Peptides ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Desorption/Ionization Efficiency of Peptides Containing Disulfide Bonds in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

2012 ◽  
Vol 28 (3) ◽  
pp. 295 ◽  
Author(s):  
Yasushi SHIGERI ◽  
Satoshi INAZUMI ◽  
Yoshihisa HAGIHARA ◽  
Akikazu YASUDA ◽  
Hideya KAWASAKI ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Disulfide Bonds ◽  
Laser Desorption ◽  
Ionization Efficiency ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Synthesis and Matrix Properties of α-Cyano-5-phenyl-2,4-pentadienic Acid (CPPA) for Intact Proteins Analysis by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 6054
Author(s):  
Antonio Monopoli ◽  
Angelo Nacci ◽  
Tommaso R. I. Cataldi ◽  
Cosima D. Calvano
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Protein Analysis ◽  
Ionization Mass Spectrometry ◽  
Intact Protein ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

The effectiveness of a synthesized matrix, α-cyano-5-phenyl-2,4-pentadienic acid (CPPA), for protein analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in complex samples such as foodstuff and bacterial extracts, is demonstrated. Ultraviolet (UV) absorption along with laser desorption/ionization mass spectrometry (LDI-MS) experiments were systematically conducted in positive ion mode under standard Nd:YLF laser excitation with the aim of characterizing the matrix in terms of wavelength absorption and proton affinity. Besides, the results for standard proteins revealed that CPPA significantly enhanced the protein signals, reduced the spot-to-spot variability and increased the spot homogeneity. The CPPA matrix was successful employed to investigate intact microorganisms, milk and seed extracts for protein profiling. Compared to conventional matrices such as sinapinic acid (SA), α-cyano-4-hydroxycinnamic acid (CHCA) and 4-chloro-α-cyanocinnamic acid (CClCA), CPPA exhibited better signal-to-noise (S/N) ratios and a uniform response for most examined proteins occurring in milk, hazelnut and in intact bacterial cells of E. coli. These findings not only provide a reactive proton transfer MALDI matrix with excellent reproducibility and sensitivity, but also contribute to extending the battery of useful matrices for intact protein analysis.

Derivatization of small biomolecules for optimized matrix-assisted laser desorption/ionization mass spectrometry

2002 ◽  
Vol 37 (9) ◽  
pp. 963-973 ◽  
Author(s):  
Andreas Tholey ◽  
Christoph Wittmann ◽  
Min-Jung Kang ◽  
Ditte Bungert ◽  
Klaus Hollemeyer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Small Biomolecules ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption
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