Bundling of axons through a capillary alginate gel enhances the detection of axonal action potentials using microelectrode arrays

AbstractWe developed a method to non-invasively detect synaptic relationships among neurons from in vitro networks. Our method uses microelectrode arrays on which neurons are cultured and from which propagation of extracellular action potentials (eAPs) in single axons are recorded at multiple electrodes. Detecting eAP propagation bypasses ambiguity introduced by spike sorting. Our methods identify short latency spiking relationships between neurons with properties expected of synaptically coupled neurons, namely they were recapitulated by direct stimulation and were sensitive to changing the number of active synaptic sites. Our methods enabled us to assemble a functional subset of neuronal connectivity in our cultures.

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All-inkjet-printed gold microelectrode arrays for extracellular recording of action potentials

Flexible and Printed Electronics ◽

10.1088/2058-8585/aa7928 ◽

2017 ◽

Vol 2 (3) ◽

pp. 035003 ◽

Cited By ~ 20

Author(s):

Bernd Bachmann ◽

Nouran Y Adly ◽

Jan Schnitker ◽

Alexey Yakushenko ◽

Philipp Rinklin ◽

...

Keyword(s):

Action Potentials ◽

Microelectrode Arrays ◽

Extracellular Recording ◽

Gold Microelectrode

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Boron-Doped Nanocrystalline Diamond Microelectrode Arrays Monitor Cardiac Action Potentials

Advanced Healthcare Materials ◽

10.1002/adhm.201300062 ◽

2013 ◽

Vol 3 (2) ◽

pp. 283-289 ◽

Cited By ~ 37

Author(s):

Vanessa Maybeck ◽

Robert Edgington ◽

Alexandre Bongrain ◽

Joseph O. Welch ◽

Emanuel Scorsone ◽

...

Keyword(s):

Action Potentials ◽

Microelectrode Arrays ◽

Nanocrystalline Diamond ◽

Boron Doped ◽

Cardiac Action

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Skeletal myotube integration with planar microelectrode arrays in vitro for spatially selective recording and stimulation: A comparison of neuronal and myotube extracellular action potentials

Biotechnology Progress ◽

10.1002/btpr.609 ◽

2011 ◽

Vol 27 (3) ◽

pp. 891-895 ◽

Cited By ~ 10

Author(s):

Christopher G. Langhammer ◽

Melinda K. Kutzing ◽

Vincent Luo ◽

Jeffrey D. Zahn ◽

Bonnie L. Firestein

Keyword(s):

Action Potentials ◽

Microelectrode Arrays

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Nanovolcano microelectrode arrays: toward long-term on-demand registration of transmembrane action potentials by controlled electroporation

Microsystems & Nanoengineering ◽

10.1038/s41378-020-0178-7 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Benoît X. E. Desbiolles ◽

Etienne de Coulon ◽

Nicolas Maïno ◽

Arnaud Bertsch ◽

Stephan Rohr ◽

...

Keyword(s):

Action Potentials ◽

Microelectrode Arrays ◽

On Demand ◽

Transmembrane Action Potentials

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Visual Response Properties in the Three Layer Turtle Visual Cortex

10.1101/131805 ◽

2017 ◽

Author(s):

Mahmood S. Hoseini ◽

Jeff Pobst ◽

Nathaniel C. Wright ◽

Wesley Clawson ◽

Woodrow Shew ◽

...

Keyword(s):

Action Potentials ◽

Receptive Fields ◽

Microelectrode Arrays ◽

Field Potential ◽

Visual Response ◽

Dorsal Cortex ◽

Spatial And Temporal Patterns ◽

Cortical Areas ◽

Neural Ensembles ◽

Evoked Activity

ABSTRACTTurtle dorsal cortex provides us with unique insights into cortical processing. It is known to share many features with the mammalian hippocampus and olfactory cortex as well as geniculo-cortical areas in stem amniotes from which mammals evolved. To this end, we have used data from extracellular recordings from microelectrode arrays to study spatial and temporal patterns of responses to visual stimuli as seen in both local field potential and action potentials. We discovered surprisingly large receptive fields, responsiveness to a broad range of stimuli, and high correlation between distant neural ensembles across recording array. Moreover, we found significant response variability regarding latency and strength in the presence of adaptation to both ongoing and visually evoked activity.

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