Cryogenic Solvents Inhibit the Binding of the Escherichia coli Heat-Stable Enterotoxin to Intestinal Brush Border Membranes

1993 ◽  
Vol 304 (2) ◽  
pp. 338-344 ◽  
Author(s):  
L.C. Katwa ◽  
C.D. Parker ◽  
J.K. Dybing ◽  
A.A. White
Keyword(s):  
Escherichia Coli ◽  
Brush Border ◽  
Brush Border Membranes ◽  
Heat Stable ◽  
Intestinal Brush Border

scholarly journals Interaction of Escherichia coli K88 antigen with porcine intestinal brush border membranes

1980 ◽  
Vol 29 (3) ◽  
pp. 897-901
Author(s):  
M J Anderson ◽  
J S Whitehead ◽  
Y S Kim
Keyword(s):  
Escherichia Coli ◽  
Brush Border ◽  
Binding Assay ◽  
Polyacrylamide Gel Electrophoresis ◽  
E Coli ◽  
Brush Border Membranes ◽  
Intestinal Epithelia ◽  
Intestinal Brush Border ◽  
Differential Filtration ◽  
Formation Of Complexes

The fimbria-associated Escherichia coli antigen, K88, was purified to homogeneity as determined by polyacrylamide gel electrophoresis and immunodiffusion. This polymeric antigen consists of noncovalently linked subunits, containing little or no carbohydrate, and has a monomeric molecular weight of 23,000. When a binding assay employing differential filtration was used, K88 formed complexes with isolated porcine intestinal brush border membranes. The formation of complexes was inhibited by glycoproteins with terminal N-acetylglucosamine and N-acetylgalactosamine residues and to a lesser extent by free N-acetylhexosamines. These amino sugars may play a role in the interaction of this pathogenic strain of E. coli with the intestinal epithelia of pigs.

scholarly journals γ-glutamyltranspeptidase in intestinal brush border membranes

FEBS Letters ◽  
1975 ◽  
Vol 58 (1-2) ◽  
pp. 230-233 ◽  
Author(s):  
Norman P. Curthoys ◽  
Richard Shapiro
Keyword(s):  
Brush Border ◽  
Brush Border Membranes ◽  
Intestinal Brush Border
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