Accelerated Growth of Hepatocytes in Association with Up-Regulation of Cyclin E in Transgenic Mice Expressing the Dominant Negative Form of Retinoic Acid Receptor

2000 ◽  
Vol 278 (1) ◽  
pp. 229-235 ◽  
Author(s):  
Atsushi Tsutusmi ◽  
Goshi Shiota ◽  
Hidetoshi Yamazaki ◽  
Takahiro Kunisada ◽  
Tadashi Terada ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Transgenic Mice ◽  
Retinoic Acid Receptor ◽  
Cyclin E ◽  
Dominant Negative ◽  
Acid Receptor ◽  
Dominant Negative Form ◽  
Accelerated Growth ◽  
Negative Form

scholarly journals Retinoic acid receptor ? dominant negative form causes steatohepatitis and liver tumors in transgenic mice

Hepatology ◽  
2004 ◽  
Vol 40 (2) ◽  
pp. 366-375 ◽  
Author(s):  
Atsushi Yanagitani ◽  
Sadako Yamada ◽  
Sakiko Yasui ◽  
Takashi Shimomura ◽  
Rie Murai ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Transgenic Mice ◽  
Liver Tumors ◽  
Retinoic Acid Receptor ◽  
Dominant Negative ◽  
Acid Receptor ◽  
Dominant Negative Form ◽  
Negative Form

scholarly journals Akt phosphorylates and suppresses the transactivation of retinoic acid receptor α

2006 ◽  
Vol 395 (3) ◽  
pp. 653-662 ◽  
Author(s):  
Harish Srinivas ◽  
Dianren Xia ◽  
Nicole L. Moore ◽  
Ivan P. Uray ◽  
Heetae Kim ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
Suppressive Effect ◽  
Dominant Negative ◽  
Nsclc Cell Line ◽  
Acid Receptor ◽  
Growth Inhibitory ◽  
Dominant Negative Form ◽  
Retinoic Acid Receptor Α ◽  
Functional Analyses

The transactivation of nuclear receptors is regulated by both ligand binding and phosphorylation. We previously showed that RARα (retinoic acid receptor α) phosphorylation by c-Jun N-terminal kinase contributes to retinoid resistance in a subset of NSCLC cells (non-small cell lung cancer cells), but the aetiology of this resistance in the remainder has not been fully elucidated [Srinivas, Juroske, Kalyankrishna, Cody, Price, Xu, Narayanan, Weigel and Kurie (2005) Mol. Cell. Biol. 25, 1054–1069]. In the present study, we report that Akt, which is constitutively activated in NSCLC cells, phosphorylates RARα and inhibits its transactivation. Biochemical and functional analyses showed that Akt interacts with RARα and phosphorylates the Ser96 residue of its DNA-binding domain. Mutation of Ser96 to alanine abrogated the suppressive effect of Akt. Overexpression of a dominant-negative form of Akt in an NSCLC cell line decreased RAR phosphorylation, increased RAR transactivation and enhanced the growth-inhibitory effects of an RAR ligand. The findings presented here show that Akt inhibits RAR transactivation and contributes to retinoid resistance in a subset of NSCLC cells.

scholarly journals Male Infertility Caused by Epididymal Dysfunction in Transgenic Mice Expressing a Dominant Negative Mutation of Retinoic Acid Receptor α1

1997 ◽  
Vol 56 (4) ◽  
pp. 985-990 ◽  
Author(s):  
Stacy L. Costa ◽  
Kim Boekelheide ◽  
Barbara C. Vanderhyden ◽  
Rahul Seth ◽  
Michael W. McBurney
Keyword(s):  
Retinoic Acid ◽  
Male Infertility ◽  
Transgenic Mice ◽  
Retinoic Acid Receptor ◽  
Dominant Negative ◽  
Acid Receptor ◽  
Dominant Negative Mutation
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