Cross-Resistance to Bacillus thuringiensis Toxin Cry1Ja in a Strain of Diamondback Moth Adapted to Artificial Diet

2000 ◽  
Vol 76 (1) ◽  
pp. 81-83 ◽  
Author(s):  
Bruce E. Tabashnik ◽  
Kenneth W. Johnson ◽  
James T. Engleman ◽  
James A. Baum
Keyword(s):  
Bacillus Thuringiensis ◽  
Artificial Diet ◽  
Diamondback Moth ◽  
Cross Resistance ◽  
Bacillus Thuringiensis Toxin

scholarly journals Cross-Resistance and Stability of Resistance to Bacillus thuringiensis Toxin Cry1C in Diamondback Moth

2001 ◽  
Vol 67 (7) ◽  
pp. 3216-3219 ◽  
Author(s):  
Yong-Biao Liu ◽  
Bruce E. Tabashnik ◽  
Susan K. Meyer ◽  
Neil Crickmore
Keyword(s):  
Bacillus Thuringiensis ◽  
Plutella Xylostella ◽  
Resistant Strain ◽  
Diamondback Moth ◽  
Field Population ◽  
Cross Resistance ◽  
Bacillus Thuringiensis Toxin ◽  
Stability Of Resistance ◽  
Resistant Strains ◽  
Reported Data

ABSTRACT We tested toxins of Bacillus thuringiensis against larvae from susceptible, Cry1C-resistant, and Cry1A-resistant strains of diamondback moth (Plutella xylostella). The Cry1C-resistant strain, which was derived from a field population that had evolved resistance to B. thuringiensis subsp.kurstaki and B. thuringiensis subsp.aizawai, was selected repeatedly with Cry1C in the laboratory. The Cry1C-resistant strain had strong cross-resistance to Cry1Ab, Cry1Ac, and Cry1F, low to moderate cross-resistance to Cry1Aa and Cry9Ca, and no cross-resistance to Cry1Bb, Cry1Ja, and Cry2A. Resistance to Cry1C declined when selection was relaxed. Together with previously reported data, the new data on the cross-resistance of a Cry1C-resistant strain reported here suggest that resistance to Cry1A and Cry1C toxins confers little or no cross-resistance to Cry1Bb, Cry2Aa, or Cry9Ca. Therefore, these toxins might be useful in rotations or combinations with Cry1A and Cry1C toxins. Cry9Ca was much more potent than Cry1Bb or Cry2Aa and thus might be especially useful against diamondback moth.

Different Cross-Resistance Patterns in the Diamondback Moth (Lepidoptera: Plutellidae) Resistant to Bacillus thuringiensis Toxin Cry1C

2001 ◽  
Vol 94 (6) ◽  
pp. 1547-1552 ◽  
Author(s):  
Jian-Zhou Zhao ◽  
Ya-Xin LI ◽  
Hilda L. Collins ◽  
Jun Cao ◽  
Elizabeth D. Earle ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Diamondback Moth ◽  
Cross Resistance ◽  
Resistance Patterns

scholarly journals Cross-resistance of the diamondback moth indicates altered interactions with domain II of Bacillus thuringiensis toxins.

1996 ◽  
Vol 62 (8) ◽  
pp. 2839-2844 ◽  
Author(s):  
B E Tabashnik ◽  
T Malvar ◽  
Y B Liu ◽  
N Finson ◽  
D Borthakur ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Diamondback Moth ◽  
Cross Resistance

scholarly journals Independent and Synergistic Effects of Knocking out Two ABC Transporter Genes on Resistance to Bacillus thuringiensis Toxins Cry1Ac and Cry1Fa in Diamondback Moth

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 9
Author(s):  
Shan Zhao ◽  
Dong Jiang ◽  
Falong Wang ◽  
Yihua Yang ◽  
Bruce E. Tabashnik ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Abc Transporter ◽  
Insect Pests ◽  
Synergistic Effects ◽  
Diamondback Moth ◽  
Cross Resistance ◽  
Vegetable Crops ◽  
Double Knockout ◽  
Bt Toxins ◽  
Abc Transporter Genes

Insecticidal proteins from Bacillus thuringiensis (Bt) are used widely in sprays and transgenic crops to control insect pests. However, evolution of resistance by pests can reduce the efficacy of Bt toxins. Here we analyzed resistance to Bt toxins Cry1Ac and Cry1Fa in the diamondback moth (Plutella xylostella), one of the world’s most destructive pests of vegetable crops. We used CRISPR/Cas9 gene editing to create strains with knockouts of the ATP-binding cassette (ABC) transporter genes PxABCC2, PxABCC3, or both. Bioassay results show that knocking out either gene alone caused at most 2.9-fold resistance but knocking out both caused >10,320-fold resistance to Cry1Ac and 380-fold resistance to Cry1Fa. Cry1Ac resistance in the double knockout strain was recessive and genetically linked with the PxABCC2/PxABCC3 loci. The results provide insight into the mechanism of cross-resistance to Cry1Fa in diamondback moth. They also confirm previous work with this pest showing that mutations disrupting both genes cause higher resistance to Cry1Ac than mutations affecting either PxABCC2 or PxABCC3 alone. Together with previous work, the results here highlight the value of using single and multiple gene knockouts to better understand the independent and synergistic effects of putative Bt toxin receptors on resistance to Bt toxins.

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