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2022 ◽  
Vol 12 ◽  
Author(s):  
Xianjun Feng ◽  
Jiajun Ma ◽  
Zhiqian Liu ◽  
Xuan Li ◽  
Yinghua Wu ◽  
...  

Glucosinolates (GSLs) are important secondary metabolites that play important defensive roles in cruciferous plants. Chinese flowering cabbage, one of the most common vegetable crops, is rich in GSLs and thus has the potential to reduce the risk of cancer in humans. Many genes that are involved in GSL biosynthesis and metabolism have been identified in the model plant Arabidopsis thaliana; however, few studies investigated the genes related to GSL biosynthesis and metabolism in Chinese flowering cabbage. In the present study, the GSL composition and content in three different organs of Chinese flowering cabbage (leaf, stalk, and flower bud) were determined. Our results showed that the total GSL content in flower buds was significantly higher than in stalks and leaves, and aliphatic GSLs were the most abundant GSL type. To understand the molecular mechanisms underlying the variations of GSL content, we analyzed the expression of genes encoding enzymes involved in GSL biosynthesis and transport in different tissues of Chinese flowering cabbage using RNA sequencing; the expression levels of most genes were found to be consistent with the pattern of total GSL content. Correlation and consistency analysis of differentially expressed genes from different organs with the GSL content revealed that seven genes (Bra029966, Bra012640, Bra016787, Bra011761, Bra006830, Bra011759, and Bra029248) were positively correlated with GSL content. These findings provide a molecular basis for further elucidating GSL biosynthesis and transport in Chinese flowering cabbage.


Nitrogen ◽  
2022 ◽  
Vol 3 (1) ◽  
pp. 26-42
Author(s):  
Jacynthe Dessureault-Rompré ◽  
Alexis Gloutney ◽  
Jean Caron

Few conservation strategies have been applied to cultivated peatland. This field study over one growth cycle of Lactuca sativa examined the effect of plant-based, high-C/N-ratio amendments in a real farming situation on peatland. Plant Root Simulator (PRS®) probes were used directly in the field to assess the impacts of incorporating Miscanthus x giganteus straw and Salix miyabeana chips on nutrient availability for lettuce. The results showed that lettuce yield decreased by 35% in the miscanthus straw treatment and by 14% in the willow chip treatment. In addition, the nitrogen flux rate was severely reduced during crop growth (75% reduction) and the plant N uptake index was much lower in the amended treatments than in the control. The phosphorus supply rate was also significantly lower (24% reduction) in the willow treatment. The influence of sampling zone was significant as well, with most macro-nutrients being depleted in the root zone and most micro-nutrients being mobilized. Additional work is needed to optimize the proposed conservation strategy and investigate the effects of consecutive years of soil amendment on different vegetable crops and in different types of cultivated peatlands to confirm and generalize the findings of this study. Future field studies should also explore the long-term carbon dynamics under plant-based, high-C/N-ratio amendments to determine if they can offset annual C losses.


2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Alassane Ouattara ◽  
Fidèle Tiendrébéogo ◽  
Nathalie Becker ◽  
Cica Urbino ◽  
Gaël Thébaud ◽  
...  

AbstractIn recent decades, a legion of monopartite begomoviruses transmitted by the whitefly Bemisia tabaci has emerged as serious threats to vegetable crops in Africa. Recent studies in Burkina Faso (West Africa) reported the predominance of pepper yellow vein Mali virus (PepYVMLV) and its frequent association with a previously unknown DNA-B component. To understand the role of this DNA-B component in the emergence of PepYVMLV, we assessed biological traits related to virulence, virus accumulation, location in the tissue and transmission. We demonstrate that the DNA-B component is not required for systemic movement and symptom development of PepYVMLV (non-strict association), but that its association produces more severe symptoms including growth arrest and plant death. The increased virulence is associated with a higher viral DNA accumulation in plant tissues, an increase in the number of contaminated nuclei of the phloem parenchyma and in the transmission rate by B. tabaci. Our results suggest that the association of a DNA-B component with the otherwise monopartite PepYVMLV is a key factor of its emergence.


2022 ◽  
pp. 19-22
Author(s):  
В.А. Борисов ◽  
И.И. Вирченко ◽  
Е.В. Янченко ◽  
О.Н. Успенская

Цветная капуста – одна из наиболее ценных овощных культур по своим питательным и лечебным свойствам. Эта культура отличается высоким содержанием полноценного растительного белка, ее используют как диетический продукт при диабете и заболеваниях желудка, кишечника, печени. Однако выращивание цветной капусты сопряжено с большими проблемами ввиду очень высокой требовательности этой культуры к уровню плодородия почвы, элементам минерального питания, влажности почвы и воздуха. В почвенно-климатических условиях ЦНЗ России уровень урожайности цветной капусты колеблется в пределах 10–20 т/га, в среднем по России – 9,7 т/га, что не всегда компенсирует затраты на ее выращивание. Важнейшее условие повышения урожайности и рентабельности этой культуры – оптимизация питательного режима и внедрение новых высокоурожайных сортов и гибридов. Цель работы: поиск способов оптимизации питания капусты цветной с помощью органических удобрений (компостов) и их комбинаций с минеральными удобрениями. В 2020–2021 годах отделом земледелия и агрохимии ВНИИО-филиале ФГБНУ ФНЦО были проведены комплексные исследования по изучению отзывчивости некоторых сортов и гибридов цветной капусты на применение органических (птичий компост в дозе 6 т/га) и минеральных (N120P120K180) удобрений и их сочетаний, а также по влиянию этих удобрений на качество продукции. Было изучено семь сортов и гибридов капусты цветной отечественной и зарубежной селекции. Результаты исследований показали, что совместное применение минеральных и органических удобрений в наибольшей степени увеличивает продуктивность капусты, в среднем на 30%, в то время как чисто минеральные – на 25%, а чисто органические – на 19%. Совместное применение минеральных и органических удобрений позволяет получать в среднем 28,3 т/га головок цветной капусты, по отдельным сортам и гибридам – от 23,9 до 38,1 т/га. Качество продукции остается хорошим при применении минеральных и органических удобрений как раздельным, так и совместным способами. Cauliflower is one of the most valuable vegetable crops for its nutritional and medicinal properties. Differs in a high content of complete vegetable protein, is a dietary product for diseases of the stomach, intestines, liver and diabetes. However, the cultivation of cauliflower is fraught with great problems due to the very high demands of this crop on the level of soil fertility, elements of mineral nutrition, soil and air moisture. In the soil and climatic conditions of the Central Black Earth Region of Russia, the yield level of cauliflower fluctuates between 10–20 t/ha, on average in Russia 9.7 t/ha, which does not always compensate for the costs of its cultivation. The most important condition for increasing the yield and profitability of this crop is the optimization of the nutritional regime and the introduction of new high-yielding varieties and hybrids. The aim of our work was to find ways to optimize the nutrition of cauliflower using organic fertilizers – composts and their combinations with mineral fertilizers. In this regard, in 2020–2021, the Department of Agriculture and Agrochemistry of the ARRIVG-branch FSBSI FSVC conducted comprehensive studies to study the responsiveness of some varieties and hybrids of cauliflower to the use of organic (bird compost at a dose of 6 t/ha) and mineral (N120P120K180) fertilizers and their combinations, as well as the effect of these fertilizers on product quality. Seven varieties and hybrids of cauliflower of domestic and foreign selection were studied. The research results showed that the combined use of mineral and organic fertilizers to the greatest extent increases the productivity of cabbage, on average by 30%, while purely mineral fertilizers – by 25%, and purely organic – by 19%. The combined use of mineral and organic fertilizers allows to obtain an average of 28.3 t/ha of cauliflower heads; for individual varieties and hybrids from 23.9 to 38.1 t/ha. Product quality remains good when mineral and organic fertilizers are used both separately and jointly.


2022 ◽  
pp. 29-32
Author(s):  
Ш.Р. Арипова ◽  
С.И. Дусмуратова

Изучение кабачка (Cucurbita pepo var. giraumontia) в природно-климатических условиях Узбекистана и совершенствование технологии выращивания семенных плодов – важное направление селекционной работы по обогащению сортимента овощных культур и организации семеноводства этой культуры. Цель исследований: установить оптимальные сроки высева семян и посадки рассады перспективной линии короткоплетистой формы кабачка LZ-2513 при весеннем сроке выращивания на семенные цели. Исследования проводили в 2016 и 2019 годах на экспериментальном участке Научно-исследовательского института овощебахчевых культур и картофеля Республики Узбекистан (Ташкентский район Ташкентской области). Климат региона схож с условиями большинства овощеводческих хозяйств, расположенных в равнинной центральной части Узбекистана. Почвы опытного участка – типичные суглинки, окультуренные, влагоемкие, глубина залегания грунтовых вод – более 6–7 м, количество гумуса в почве – 0,641–0,943%, азота – 0,072–0,121%, фосфора – 0,130–0,171% и калия – 1,627–2,206%. Погодные условия 2016 и 2019 годов были благоприятными для роста и развития кабачка, за исключением засушливого июля и августа, в течение которых недостаток влаги компенсировали поливами. Испытаны четыре весенних срока посева и посадки рассады (в 2016 году – 3 апреля, 12 апреля, 20 апреля и 1 мая; в 2019 году – 1 апреля, 10 апреля, 21 апреля и 30 апреля). Делянки двухрядковые, длиной 10 м, схема размещения (140+70)/2×50 см. Площадь учетной делянки 21,0 м2. На каждой делянке размещались по 40 растений, повторность опыта четырехкратная. Установлено, что в условиях Ташкентской области Узбекистана наибольший выход семян из плодов у линии LZ-2513 был при выращивании кабачка рассадным способом в первой декаде апреля (в начале I и II декады апреля), а при безрассадной культуре – при посеве семян в начале апреля, что связано с наиболее благоприятными температурными условиями для культуры кабачка. The study of squash (Cucurbita pepo var. giraumontia) in the natural and climatic conditions of Uzbekistan and the improvement of the technology of growing seed fruits is an important direction of breeding work is to enrich the assortment of vegetable crops and the organization of seed production of this crop. The purpose of the research: to establish the optimal timing of sowing seeds and planting seedlings of a promising line of short-leafed squash LZ-2513 at the spring period of cultivation for seed purposes. The research was carried out in 2016 and 2019 at the experimental site of the Scientific Research Institute of Vegetable and melon crops and Potato of the Republic of Uzbekistan (Tashkent district of the Tashkent region). The climate of the region is similar to the conditions of most vegetable farms located in the flat central part of Uzbekistan. The soils of the experimental site are typical loams, cultivated, moisture-intensive, the depth of groundwater is more than 6–7 meters, the amount of humus in the soil is 0.641–0.943%, nitrogen – 0.072–0.121%, phosphorus – 0.130–0.171% and potassium – 1.627–2.206%. Weather conditions in 2016 and 2019 were favorable for the growth and development of squash, with the exception of dry July and August, during which the lack of moisture was compensated by watering. 4 spring sowing and planting dates have been tested (in 2016 – April 3, April 12, April 20 and May 1; in 2019 – April 1, April 10, April 21 and April 30). Two-row plots, 10 m long, layout (140+70)/2×50 cm. The area of the accounting plot is 21.0 m2. 40 plants were placed on each plot, the repetition of the experiment was fourfold. It was found that in the conditions of the Tashkent region of Uzbekistan, the highest yield of seeds from fruits of the LZ-2513 line was established when squash was grown by seedling method in the first decade of April (at the beginning of the first and second decade of April), and with seedless culture – when sowing seeds in early April, which is associated with the most favorable temperature conditions for squash culture.


Author(s):  
D. Miljaković ◽  
J. Marinković ◽  
G. Tamindžić ◽  
V. Đorđević ◽  
M. Ignjatov ◽  
...  

Background: Bacillus spp., known to promote growth and reduce disease of various field and vegetable crops, are frequently found in soils. The objective of the study was to select effective Bacillus spp. isolates with multiple plant growth properties and antifungal activities and to examine their effect on germination of soybean. Methods: Bacterial isolates were screened for production of indole-3-acetic acid (IAA) and siderophores and solubilization of phosphate. The ability of bacterial isolates to inhibit the growth of seven phytopathogenic fungi affecting soybean was determined using a dual plate assay. Bacillus spp. were further selected and examined in a seed germination test. Result: All Bacillus spp. isolates were positive for IAA production, while siderophore production and P-solubilization were observed in 80% and 20% bacterial isolates, respectively. Bacillus spp. exhibited the highest antifungal activity against Diaporthe caulivora, followed by Diaporthe sojae, Diaporthe eres, Diaporthe longicolla and Macrophomina phaseolina and the least antagonistic effect toward Fusarium graminearum and Fusarium subglutinans. Selected isolates of B. subtilis significantly affected final germination, shoot length, root length, shoot dry weight and root dry weight of two soybean cultivars. The most effective Bacillus spp. isolates could be used as potential inoculants for improving soybean productivity.


Author(s):  
Karima Sabri ◽  
Aissa Mokabli ◽  
Fouad Mokrini ◽  
Slimane Khayi ◽  
Salah-Eddine Laasli ◽  
...  

EDIS ◽  
2022 ◽  
Vol 2021 (6) ◽  
Author(s):  
Rao Mylavarapu ◽  
George Hochmuth ◽  
Guodong Liu

This publication presents the fertilization recommendations for vegetable crops based on soil tests performed by the UF/IFAS Extension Soil Testing Laboratory (ESTL). It contains the basic information from which ESTL soil test reports and fertilization recommendations are generated. The audiences for this information include commercial and small farmers, crop advisers and consultants, state and local agencies, fertilizer industry, and any interested individuals interested in sustainable nutrient and environmental management. Major revision by Rao Mylavarapu, George Hochmuth, and Guodong Liu; 12 pp. https://edis.ifas.ufl.edu/cv002


Electronics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 140
Author(s):  
Hesham Tarek ◽  
Hesham Aly ◽  
Saleh Eisa ◽  
Mohamed Abul-Soud

Smart agriculture has taken more attention during the last decade due to the bio-hazards of climate change impacts, extreme weather events, population explosion, food security demands and natural resources shortage. The Egyptian government has taken initiative in dealing with plants diseases especially tomato which is one of the most important vegetable crops worldwide that are affected by many diseases causing high yield loss. Deep learning techniques have become the main focus in the direction of identifying tomato leaf diseases. This study evaluated different deep learning models pre-trained on ImageNet dataset such as ResNet50, InceptionV3, AlexNet, MobileNetV1, MobileNetV2 and MobileNetV3.To the best of our knowledge MobileNetV3 has not been tested on tomato leaf diseases. Each of the former deep learning models has been evaluated and optimized with different techniques. The evaluation shows that MobileNetV3 Small has achieved an accuracy of 98.99% while MobileNetV3 Large has achieved an accuracy of 99.81%. All models have been deployed on a workstation to evaluate their performance by calculating the prediction time on tomato leaf images. The models were also deployed on a Raspberry Pi 4 in order to build an Internet of Things (IoT) device capable of tomato leaf disease detection. MobileNetV3 Small had a latency of 66 ms and 251 ms on the workstation and the Raspberry Pi 4, respectively. On the other hand, MobileNetV3 Large had a latency of 50 ms on the workstation and 348 ms on the Raspberry Pi 4.


2022 ◽  
Vol 3 (1) ◽  
Author(s):  
Robert L. Meagher ◽  
Rodney N. Nagoshi ◽  
Shelby J. Fleischer ◽  
John K. Westbrook ◽  
David L. Wright ◽  
...  

Abstract Background Fall armyworm, Spodoptera frugiperda (J. E. Smith) is a migratory moth that annually migrates northward each spring from sites in southern Florida and southern Texas. This caterpillar pest feeds on and damages row, turf and vegetable crops in the eastern and central U.S. Before migrating in spring, it feeds on cover crops in central and eastern Florida and expands its populations. Our objective was to use multi-year studies to compare fall armyworm populations that develop in cover crop plants. Methods A series of field experiments and a laboratory feeding study were conducted to compare infestation and feeding and of fall armyworm on different cover crop plants. Field experiments had plots planted with corn (Zea mays L.), sorghum-sudangrass [Sorghum bicolor (L.) Moench], a standard cover crop in Florida, and two alternative cover crops, sunn hemp (Crotalaria juncea L.) and cowpea [Vigna unguiculata (L.) Walpers spp. unguiculata]. Another trial compared populations in sorghum-sudangrass and in mixtures of sorghum-sudangrass with buckwheat (Fagopyrum esculentum Moench) or pearl millet (Cenchrus americanus (L.) Morrone). Fall armyworm larvae were fed and allowed to develop on different sunn hemp germplasm in a laboratory trial. Results Field populations of fall armyworm were highest on corn, followed by sorghum-sudangrass. Sunn hemp and cowpea had larval populations 70–96% less than on sorghum-sudangrass, suggesting replacement of this cover crop with either plant species might help reduce areawide populations of resident or migratory fall armyworm. Larvae collected from cover crop plots had parasitism levels that averaged 30%, with Chelonus insularis (Hymenoptera: Braconidae) emerging as the most commonly-collected species. Larval feeding on different sunn hemp germplasm lines resulted in no difference in weight gain. Conclusions Replacing sorghum-sudangrass with sunn hemp varieties or germplasm should be acceptable as a replacement cover crop for areawide management of fall armyworm.


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