Appendix: Model Studies Relating Nuclear Magnetic Resonance Data with the Three-dimensional Structure of Protein-DNA Complexes

1993 ◽  
Vol 234 (4) ◽  
pp. 1094 ◽  
Author(s):  
Martin Billeter ◽  
Kurt Wüthrich
Author(s):  
A.N. Sagredos ◽  
R. Moser

AbstractBased on previous work (7) a method to simultaneously determine vamidothion [I], vamidothion-sulfoxide [II] and vamidothion sulfone [III] in tobacco has been developed. The compounds are extracted with water/acetone/acetic acid from the tobacco, cleansed over an aluminium oxide column and then determined on the gas chromatograph with the specific sulphur detector. Rates of recovery are 70 % - 92 %, the determination level is 0.1 ppm. Mass spectrometry and nuclear magnetic resonance data of vamidothion [I], vamidothion-sulfoxide [ II ] and vamidothion-sulfone [III] are given.


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