Crystallization and Preliminary X-ray Analysis of Restriction Endonuclease BamHI-DNA Complex

1994 ◽  
Vol 239 (3) ◽  
pp. 430-432 ◽  
Author(s):  
T. Strzelecka ◽  
M. Newman ◽  
L.F. Dorner ◽  
R. Knott ◽  
I. Schildkraut ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
X Ray ◽  
Restriction Endonuclease Bamhi ◽  
Dna Complex

Preliminary X-ray diffraction studies of EcoRI restriction endonuclease-DNA complex

1981 ◽  
Vol 145 (3) ◽  
pp. 607-610 ◽  
Author(s):  
Ti-Sheng Young ◽  
Sung-Hou Kim ◽  
Paul Modrich ◽  
Akum Beth ◽  
Ernest Jay
Keyword(s):  
Restriction Endonuclease ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ecori Restriction ◽  
Dna Complex

scholarly journals X-ray Structure of a NF-κB p50/RelB/DNA Complex Reveals Assembly of Multiple Dimers on Tandem κB Sites

2007 ◽  
Vol 373 (3) ◽  
pp. 723-734 ◽  
Author(s):  
Anu K. Moorthy ◽  
De-Bin Huang ◽  
Vivien Ya-Fan Wang ◽  
Don Vu ◽  
Gourisankar Ghosh
Keyword(s):  
X Ray ◽  
Dna Complex

Crystallization of Restriction Endonuclease BamHI with Nonspecific DNA

2000 ◽  
Vol 130 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Hector Viadiu ◽  
Rebecca Kucera ◽  
Ira Schildkraut ◽  
Aneel K. Aggarwal
Keyword(s):  
Restriction Endonuclease ◽  
Restriction Endonuclease Bamhi

scholarly journals Crystallization and preliminary X-ray characterization of the eukaryotic replication terminator Reb1–Ter DNA complex

2015 ◽  
Vol 71 (4) ◽  
pp. 414-418 ◽  
Author(s):  
Rahul Jaiswal ◽  
Samarendra K. Singh ◽  
Deepak Bastia ◽  
Carlos R. Escalante
Keyword(s):  
Replication Fork ◽  
Structural Information ◽  
Transcription Termination ◽  
Replication Forks ◽  
Unit Cell Parameters ◽  
X Ray ◽  
Cell Parameters ◽  
Naturally Occurring ◽  
Dna Complex

The Reb1 protein fromSchizosaccharomyces pombeis a member of a family of proteins that control programmed replication termination and/or transcription termination in eukaryotic cells. These events occur at naturally occurring replication fork barriers (RFBs), where Reb1 binds to termination (Ter) DNA sites and coordinates the polar arrest of replication forks and transcription approaching in opposite directions. The Reb1 DNA-binding and replication-termination domain was expressed inEscherichia coli, purified and crystallized in complex with a 26-mer DNA Ter site. Batch crystallization under oil was required to produce crystals of good quality for data collection. Crystals grew in space groupP21, with unit-cell parametersa= 68.9,b= 162.9,c= 71.1 Å, β = 94.7°. The crystals diffracted to a resolution of 3.0 Å. The crystals were mosaic and required two or three cycles of annealing. This study is the first to yield structural information about this important family of proteins and will provide insights into the mechanism of replication and transcription termination.

scholarly journals Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I

2007 ◽  
Vol 63 (9) ◽  
pp. 795-797 ◽  
Author(s):  
Galina S. Kachalova ◽  
Alfiya K. Yunusova ◽  
Rimma I. Artyukh ◽  
Eugeny A. Rogulin ◽  
Tatyana A. Perevyazova ◽  
...  
Keyword(s):  
Diffraction Analysis ◽  
Restriction Endonuclease ◽  
Small Subunit ◽  
X Ray Diffraction ◽  
X Ray
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