Variations in the Relative mRNA Levels of Actins and Myosin Heavy Chains do not Produce Corresponding Differences in their Proteins in the Adult Human Heart

1997 ◽  
Vol 29 (3) ◽  
pp. 895-905 ◽  
Author(s):  
Joëlle V.F. Coumans ◽  
Thomas Yeoh ◽  
Reginald K. Seeto ◽  
Anne Keogh ◽  
Karen Brennan ◽  
...  
Keyword(s):  
Human Heart ◽  
Mrna Levels ◽  
Myosin Heavy Chains ◽  
Heavy Chains ◽  
Adult Human

Developmental myosin heavy chains in the adult human diaphragm: coexpression patterns and effect of COPD

2000 ◽  
Vol 88 (4) ◽  
pp. 1446-1456 ◽  
Author(s):  
Taitan Nguyen ◽  
Joseph Shrager ◽  
Larry Kaiser ◽  
Lijuan Mei ◽  
Monica Daood ◽  
...  
Keyword(s):  
Forced Expiratory Volume ◽  
Fiber Formation ◽  
Chronic Obstructive ◽  
Myosin Heavy Chains ◽  
Obstructive Pulmonary Disease ◽  
Heavy Chains ◽  
Adult Human ◽  
Sds Page ◽  
And Control ◽  
Severe Copd

In preliminary experiments we noted developmental (i.e., embryonic and neonatal) myosin heavy chains (MHCs) in the diaphragms of patients with severe chronic obstructive pulmonary disease (COPD). We hypothesized that this finding represented new fiber formation secondary to injury associated with the mechanical stress of COPD or previously undescribed MHCs in the human diaphragm. To distinguish between these possibilities, we analyzed diaphragmatic biopsies obtained from 9 patients with severe COPD (forced expiratory volume in 1 s = 21 ± 2% predicted, residual volume = 283 ± 22% predicted) and 10 age-matched controls. First, using immunocytochemistry with specific monoclonal antibodies, we noted that control diaphragms had greater proportions of fibers expressing embryonic (50 ± 2 vs. 28 ± 3%, P < 0.0001) and neonatal (52 ± 2 vs. 32 ± 3%, P < 0.001) MHCs than COPD diaphragms. Second, SDS-PAGE demonstrated that these developmental MHCs represented only a very small fraction of the diaphragmatic MHC content. Third, the RT-PCR demonstrated mRNA coding for embryonic and neonatal MHCs in COPD and control diaphragms. Last, COPD and control diaphragms exhibited normal histology on light microscopy. We conclude that the presence of developmental MHC isoforms does not indicate new fiber formation in diaphragms of patients with severe COPD. Although these results represent the first systematic description of embryonic and neonatal MHCs in normal adult human diaphragms, their function remains to be elucidated.

A Computational Reconstruction of the Adult Human Heart Transcriptional Profile

2000 ◽  
Vol 32 (11) ◽  
pp. 1931-1938 ◽  
Author(s):  
Stefania Bortoluzzi ◽  
Fabio d»Alessi ◽  
Gian Antonio Danieli
Keyword(s):  
Human Heart ◽  
Transcriptional Profile ◽  
Adult Human ◽  
Computational Reconstruction

scholarly journals CD117-Positive Cells in Adult Human Heart Are Localized in the Subepicardium, and Their Activation Is Associated with Laminin-1 and α6Integrin Expression

Stem Cells ◽  
2008 ◽  
Vol 26 (7) ◽  
pp. 1723-1731 ◽  
Author(s):  
Clotilde Castaldo ◽  
Franca Di Meglio ◽  
Daria Nurzynska ◽  
Gianpaolo Romano ◽  
Ciro Maiello ◽  
...  
Keyword(s):  
Human Heart ◽  
Adult Human ◽  
Laminin 1

Monospecific Antibodies against the Three Mammalian Fast Limb Myosin Heavy Chains

2000 ◽  
Vol 272 (1) ◽  
pp. 303-308 ◽  
Author(s):  
Christine A. Lucas ◽  
Lucia H.D. Kang ◽  
Joseph F.Y. Hoh
Keyword(s):  
Myosin Heavy Chains ◽  
Heavy Chains ◽  
Monospecific Antibodies

scholarly journals Carbonylation of myosin heavy chains in rat heart during diabetes

2010 ◽  
Vol 80 (2) ◽  
pp. 205-217 ◽  
Author(s):  
Chun-Hong Shao ◽  
George J. Rozanski ◽  
Ryoji Nagai ◽  
Frank E. Stockdale ◽  
Kaushik P. Patel ◽  
...  
Keyword(s):  
Rat Heart ◽  
Myosin Heavy Chains ◽  
Heavy Chains

scholarly journals Shortening velocity and myosin heavy chains of developing rabbit muscle fibers.

1985 ◽  
Vol 260 (27) ◽  
pp. 14403-14405 ◽  
Author(s):  
P J Reiser ◽  
R L Moss ◽  
G G Giulian ◽  
M L Greaser
Keyword(s):  
Muscle Fibers ◽  
Rabbit Muscle ◽  
Myosin Heavy Chains ◽  
Shortening Velocity ◽  
Heavy Chains

Modulation of Myosin Heavy Chains in Rat Laryngeal Muscle

2001 ◽  
Vol 111 (3) ◽  
pp. 472-477 ◽  
Author(s):  
Akihiro Shiotani ◽  
Hideki Nakagawa ◽  
Paul W. Flint
Keyword(s):  
Myosin Heavy Chains ◽  
Laryngeal Muscle ◽  
Heavy Chains
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