Use of Human Skin Equivalent Apligraf for in Vitro Assessment of Cumulative Skin Irritation Potential of Topical Products

2000 ◽  
Vol 164 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Jesús Medina ◽  
Anne de Brugerolle de Fraissinette ◽  
Salah-Dine Chibout ◽  
Maryelle Kolopp ◽  
Rahel Kammermann ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Irritation ◽  
Skin Equivalent ◽  
In Vitro Assessment ◽  
Human Skin Equivalent

Desmosomal distribution in the epidermis of a non-contracting human skin equivalent

1992 ◽  
Vol 50 (1) ◽  
pp. 896-897
Author(s):  
L.X. Oakford ◽  
S.D. Dimitrijevich ◽  
R. Gracy
Keyword(s):  
Human Skin ◽  
Basal Layer ◽  
Skin Equivalent ◽  
Tissue Component ◽  
Intact Skin ◽  
Similar Sequence ◽  
Sequence Of Events ◽  
Suprabasal Keratinocytes ◽  
Human Skin Equivalent

In intact skin the epidermal layer is a dynamic tissue component which is maintained by a basal layer of mitotically active cells. The protective upper epidermis, the stratum corneum, is generated by differentiation of the suprabasal keratinocytes which eventually desquamate as anuclear comeocytes. A similar sequence of events is observed in vitro in the non-contracting human skin equivalent (HSE) which was developed in this lab (1). As a part of the definition process for this model of living skin we are examining its ultrastructural features. Since desmosomes are important in maintaining cell-cell interactions in stratified epithelia their distribution in HSE was examined.

scholarly journals Development of a Full-Thickness Human Skin Equivalent In Vitro Model Derived from TERT-Immortalized Keratinocytes and Fibroblasts

2015 ◽  
Vol 21 (17-18) ◽  
pp. 2448-2459 ◽  
Author(s):  
Christianne M.A. Reijnders ◽  
Amanda van Lier ◽  
Sanne Roffel ◽  
Duco Kramer ◽  
Rik J. Scheper ◽  
...  
Keyword(s):  
Human Skin ◽  
In Vitro Model ◽  
Skin Equivalent ◽  
Full Thickness ◽  
Vitro Model ◽  
Human Skin Equivalent

Morphological effects of sulfur mustard on a human skin equivalent

1991 ◽  
Vol 49 ◽  
pp. 78-79
Author(s):  
J.P. Petrali ◽  
S.B. Oglesby ◽  
T.A. Justus
Keyword(s):  
Human Skin ◽  
Sulfur Mustard ◽  
Athymic Nude Mouse ◽  
Skin Equivalent ◽  
Model Systems ◽  
Cells In Culture ◽  
Vitro Model ◽  
Human Skin Equivalent

We have previously reported morphological correlates of sulfur mustard (HD) toxicity in several model systems: the human skin grafted athymic nude mouse; the hairless guinea pig; and human cells in culture. We are now describing HD effects in a human skin equivalent, TESTSKIN®, and comparing these effects with those already reported for animal models and cells in culture. The human skin equivalent (HSE) is used here as an organotypic in vitro model system to bridge the knowledge gap between HD effects in monotypic cells in culture and animal in vivo effects. Additionally, HSE allowed study of HD toxicity which circumvented the concern of using human biopsied tissue.

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